In this study, the current ichthyofauna of the Seyhan, Ceyhan and Orontes River drainages were presented and actual taxonomic positions of the species were assessed. Sixty-seven species belonging to 32 genera and 17 families of fishes were reported from these river drainages in Turkey and Syria. Acanthobrama centisquama and Tinca tinca could not be observed in the study area and Alburnus sellal and Esox lucius are recorded for the first time respectively in the Lake Gölbaşı (connected to the Ceyhan River) and Seyhan River.