Biodiversity Data Journal 9: e68043 (e @)
doi: 10.3897/BDJ.9.e68043 open access
Taxonomic Paper

Two new species of the genus Anufrievia
Dworakowska from China (Hemiptera:
Cicadellidae: Typhlocybinae)

Weiwen Tan}, Jia Jiang?, Yuehua Songt

+ School of Karst Science, Guizhou Normal University / State Key Laboratory Cultivation Base for Guizhou Karst Mountain
Ecology Environment of China, Guiyang, 550001, China

Corresponding author: Yuehua Song (Ssongyuehua@163.com)

Academic editor: Colin Favret

Received: 29 Apr 2021 | Accepted: 26 May 2021 | Published: 03 Jun 2021

Citation: Tan W, Jiang J, Song Y (2021) Two new species of the genus Anufrievia Dworakowska from China
(Hemiptera: Cicadellidae: Typhlocybinae). Biodiversity Data Journal 9: e68043.
https://doi.org/10.3897/BDJ.9.e68043

ZooBank: urn:|sid:zoobank.org:pub:5A078994-03EC-4701-9CAE-931EE8FB0889

Abstract

Background

The leafhopper genus Anufrievia Dworakowska, 1970 includes 33 species and is widely
distributed in China, Korea, South Korea, Japan, Nepal, India, Thailand and Vietnam.

New information

Two new species found at Bijie City and Shibing County, Guizhou Province, China are
described and illustrated, A. crispata sp. nov. and A. confluensa sp. nov. A key to
distinguish the Chinese species of the genus is given.

Keywords

Homoptera, Auchenorrhyncha, Erythroneurini, taxonomy, leafhopper

© Tan W et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY
4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are
credited.

2 Tan W etal

Introduction

The leafhopper genus Anufrievia Dworakowska, 1970 belongs to the tribe Erythroneurini of
Typhlocybinae, with Anufrievia_ rolikae Dworakowska, 1970 as its type species
(Dworakowska 1970). The genus previously contained 33 species, including 28 species in
China (Cao et al. 2018). In this paper, two new species from Guizhou Province, China are
described and illustrated and a key to distinguish the Chinese species of the genus is
provided.

The characteristics of Anufrievia genus are as follows. Body yellow or white, often with
brown markings or diffuse patterns. Head slightly narrower than pronotum. Length of crown
distinctly shorter than inter ocular width. Body length 2.0—4.0 mm (including wing). Vertex
anterior margin with minute paired black spots. Male anteclypeus narrow and flat, greyish,
brown or black. Pronotum pale, scutellum with dark lateral triangles. Forewing with 4!
apical cell small, not reaching apex of forewing, 2™ apical cell nearly rectangular and 1°
apical cell broad. Hind wing venation follows typical schemes for Erythroneurini taxa.
Abdominal apodemes small and narrow, extended dorsomesad.

Male pygofer lobe with hind margin sleeked or truncated slightly, basal lateral angle usually
with macrosetae, sometimes absent and scattered a few fine setae in outer lateral surface.
Pygofer dorsal appendage articulated to pygofer lobe with ventral appendage absent.
Subgenital plate with some macrosetae in mid-ventral part, broad basally and sometimes
terminal half tapering abruptly; row of stout setae along upper margin from sub-base to
apex. Apex of style pointed, bifid, foot-shaped or otherwise modified. Aedeagus with dorsal
apodeme well developed; aedeagal shaft tubular; gonopore sub-basal to subapical on
ventral surface. Connective lateral arms long, Y- or V-shaped.

A

Figure 1. EES

Anufrievia crispata sp. nov. A. Habitus, dorsal view; B. Habitus, lateral view; C. Head and
thorax, dorsal view; D. Face.

Two new species of the genus Anufrievia Dworakowska from China (Hemiptera: ... 3

Materials and methods

All specimens in this study were collected by the sweeping-net method. Morphological
terminology used follows Dietrich (2005) and Dworakowska (1993). An Olympus SZX16
dissecting microscope was used for observing and an Olympus BX53 stereomicroscope for
drawing. A KEYENCE VHX-5000 digital microscope was used for taking habitus photos.
Body measurements are from the apex of the vertex to the tip of the forewing. All
specimens examined were deposited in the collection of the School of Karst Science,
Guizhou Normal University, China (GZNU).

Taxon treatments
Anufrievia crispata, sp. n.
: ZooBank 6C5F6BDB-CDAB-4407-857F-72CF40893FF7
Materials

Holotype:
a. scientificName: Anufrievia crispata; order: Hemiptera; family: Cicadellidae; genus:
Anufrievia; specificEpithet: crispata; country: China; stateProvince: Guizhou; locality: Bijie
City, Qixinguan District, Salaxi Town; |ocationRemarks: label transliteration: "Guizhou,
Bijie, 24. 10. 2019, coll. Zhouwei Yuan and Xiao Yang"; individualCount: 1; sex: male;
lifeStage: adult; collectionCode: Insects; basisOfRecord: PreservedSpecimen

Paratype:
a. scientificName: Anufrievia crispata; order: Hemiptera; family: Cicadellidae; genus:
Anufrievia; specificEpithet: crispata; country: China; stateProvince: Guizhou; locality: Bijie
City, Qixinguan District, Salaxi Town; |ocationRemarks: label transliteration: "Guizhou,
Bijie, 24.10. 2019, coll. Zhouwei Yuan and Xiao Yang"; individualCount: 4; sex: male;
lifeStage: adult; collectionCode: Insects; basisOfRecord: PreservedSpecimen

Description

Body brownish-black. Head brownish-yellow, with pair of small dark brown apical spots
(Fig. 1A and C). Eyes black. Face brownish-yellow, frontoclypeus brownish and
anteclypeus centrally brown with black lateral margins (Fig. 1B and D). Pronotum light
brownish (Fig. 1A and C). Scutellum brownish-yellow, with black basal triangles (Fig.
1A and C). Forewing beige (Fig. 1A and B). Abdominal apodemes broad, extended to 4
'N sternite (Fig. 2A). Male length 3.7—4.0 mm (including wing).

Diagnosis

Male genitalia. Pygofer lobe with numerous microsetae distributed densely along
dorsal and near posterior margin, few fine setae scattered on lateral surface (Fig. 2B).
Pygofer dorsal appendage broadened at base, tapering towards apex (Fig. 2C).
Subgenital plate robust, with three macrosetae near mid-length on lateral surface,

Tan W et al

several peg-like setae distributed from sub-base to apex; several microsetae scattered
on apical portion (Fig. 2D). Style with two points apically; pre-apical lobe prominent
(Fig. 2E). Aedeagal shaft curved dorsally, with serrated marginal lamellae on shaft; pair
of small processes curved mesally gonopore; subapical on ventral surface (Fig. 2F and
G). Connective V-shaped, slender (Fig. 2H).

Figure 2. EESl

Anufrievia crispata sp. nov. A. Abdominal apodemes; B. Pygofer lobe; C. Pygofer dorsal
appendage, lateral view; D. Subgenital plate; E. Style F. Aedeagus, ventral view; G. Aedeagus,
ventrolateral view; H. Connective.

Etymology

The new species is named from the Latin word “crispatus’, referring to the serrated
marginal lamellae on both sides of shaft apex dorsad (Fig. 2F and G).

Taxon discussion

This species can be distinguished from other species in this genus by the unique
characters of the aedeagus: the aedeagal shaft with serrated marginal lamellae on both
sides of apex, pair of small curved processes subapically; short dorsal apodeme and
long prearium.

Two new species of the genus Anufrievia Dworakowska from China (Hemiptera: ... 3

Anufrievia confluensa, sp. n.
° ZooBank 67AEODA0-127A-4D2B-9B54-F DOCD9EC78C4
Material

Holotype:

a. scientificName: Anufrievia confluensa; genus: Anufrievia; specificEpithet: confluensa;
country: China; stateProvince: Guizhou; county: Shibing; locationRemarks: label
transliteration: "Guizhou, Shibing, 24.7.2019, coll. Znouwei Yuan and Xiao Yang’;
individualCount: 1; sex: male; lifeStage: adult; collectionCode: Insects; basisOfRecord:
PreservedSpecimen

Figure 3. EESl

Anufrievia confluensa sp. nov. A. Habitus, dorsal view; B. Habitus, lateral view; C. Head and
thorax, dorsal view; D. Face.

Description

Male length 2.8 mm (including wing). Body yellowish. Vertex brownish-yellow, with pair
of small dark brown apical spots (Fig. 3A and C). Eyes grey. Face pale milky yellow,
anteclypeus and frontoclypeus light brownish (Fig. 3B and D). Pronotum and scutellum
brownish-yellow and anterior margin of scutellum with black triangles (Fig. 3A and C).
Forewing beige (Fig. 3A and B). Abdominal apodemes very short, not extended to 37
sternite (Fig. 4A).

Diagnosis

Male genitalia. Pygofer lobe broad, with dense microsetae near dorso-caudal margin
and several peg-like setae on outer surface (Fig. 4B). Pygofer dorsal appendage
broadened at base, tapering towards apex (Fig. 4C). Subgenital plate slightly concave
near middle area, with three macrosetae on lateral margin, row of short stout setae
along upper margin from sub-base to apex (Fig. 4D). Style with two points at apex; pre-

Tan W et al

apical lobe small (Fig. 4E). Aedeagal shaft straight and flat in lateral view, but long and
slim in ventral view; gonopore arising from ventral surface, reaching two thirds of
aedeagal shaft; dorsal apodeme well developed (Fig. 4F and G). Connective Y-shaped,
two arms slender, central lobe absent (Fig. 4H).

Figure 4. EES]

Anufrievia confluensa sp. nov. A. Abdominal apodemes; B. Pygofer lobe; C. Pygofer dorsal
appendage, lateral view; D. Subgenital plate; E. Style; F. Aedeagus, ventral view; G.
Aedeagus, lateral view; H. Connective.

Etymology

The new species is named from the Latin word “confluensus’, referring to the
connective stem fused with a long process (Fig. 4H).

Taxon discussion

This species is similar to A. akazu (Matsumura 1932), but can be recognised by the
subapical ventral surface without paired short processes and pygofer appendage not
bifurcate at apex.

Two new species of the genus Anufrievia Dworakowska from China (Hemiptera: ...

Identification keys

Key to males of Anufrievia from China (modified from Cao et al. 2018)

10

Pygofer dorsal appendage not bifurcate at apex
Pygofer dorsal appendage bifurcate at apex
Aedeagus with large dorsal apodeme

Aedeagus with small dorsal apodeme

Pre-atrial process not reaching gonopore

Pre-atrial process reaching or surpassing gonopore
Style without distinct apical and subapical teeth
Style with distinct apical and subapical teeth

Style with apex slim (Fig. 5T)

Style with apex triangular (Fig. 5U)

Pre-atrial process almost rectangular in ventral view,
apex broad (Fig. 50)

Pre-atrial process narrowing apically, apex pointed

Style with subapical tooth equal in length to apical tooth

(Fig. 5A)

Style with subapical tooth shorter than apical tooth (Fig.

5P)
Aedeagal shaft with pair of apical processes
Aedeagal shaft without any apical process

Aedeagal apical processes arched medially in ventral
view (Fig. 5B)

Aedeagal apical processes slightly curved in ventral view

Aedeagal shaft with base slim, slightly wider than apex

(Fig. 5X)

Aedeagal shaft with base broad, much wider than apex

11

>

. Crispata sp. nov

A. symmetrica Cao & Zhang

A. triangulata Cao & Zhang

A. quadrata Cao & Zhang

A. adaucta Cao & Zhang

A. sphenoides Yang & Zhang

A. confluensa sp. nov

A. arcuata Yang & Zhang

10

A. zelta Dworakowska

11

11

12

16

17

18

19

20

Tan W et al

Aedeagal shaft constricted sub-basally (Fig. 5J)
Aedeagal shaft not constricted sub-basally

Style with apical tooth extremely small, aedeagal shaft
straight (Fig. 5R)

Style with apical tooth relatively long, aedeagal shaft
curved dorsad

Aedeagal shaft with processes near middle (Fig. 5V)
Aedeagal shaft without process near middle

Apex of style serrated at middle

Apex of style smooth at middle

Upper tooth of pygofer dorsal appendage much shorter
than lower one (Fig. 5D)

Upper tooth of pygofer dorsal appendage subequal to or
longer than lower one

Upper tooth of pygofer dorsal appendage longer than
lower one (Fig. 5F)

Upper tooth of pygofer dorsal appendage almost as long
as lower one

Apex of pre-atrial process rounded, with one side
serrated (Fig. 5M)

Apex of pre-atrial process truncate, with both sides
smooth (Fig. 5E)

Ventral margin of aedeagal shaft protruded subapically in
lateral view

Ventral margin of aedeagal shaft straight subapically, in
lateral view

Apical tooth of style almost equal to subapical tooth (Fig.
5K)

Apical tooth of style greatly shorter than subapical tooth

Aedeagal shaft processes relatively long, gonopore
central (Fig. 5L)

A. jinghongensis Cao & Zhang

12

A. subdentata Yang & Zhang

A. ciconia Dworakowska

A. triprocessa Yang & Zhang
14
15
18

A. bauhinicola Dworakowska &
Viraktamath

16

A. expansa Cao & Zhang

17

A. plana Yang & Zhang

A. curva Yang & Zhang

19

21

A. liubanus Yang & Zhang

20

A. parisakazu Cao & Zhang

21

22

25

26

27

28

29

Two new species of the genus Anufrievia Dworakowska from China (Hemiptera: ...

Aedeagal shaft processes relatively short, gonopore
subapical

Apex of pre-atrial process serrated laterally (Fig. 51)
Apex of pre-atrial process smooth

Pre-atrial process rudimentary, as long as 1/5 of
aedeagal shaft (Fig. 5C)

Pre-atrial process much longer than 1/5 of aedeagal shaft
Aedeagal shaft curved dorsad (Fig. 5G)

Aedeagal shaft straight

Apex of style slender (Fig. 5N)

Apex of style foot-like

Aedeagal shaft with processes arising from subapex
Aedeagal shaft with processes arising from apex

Apex of aedeagal shaft expanded (Fig. 5H)

Apex of aedeagal shaft narrow (Fig. 5Q)

Aedeagal shaft processes bent at right angle in ventral
view

Aedeagal shaft processes straight or slightly curved in
ventral view

Style without distinct apical and subapical teeth (Fig. 5S)
Style with distinct apical and subapical teeth
Gonopore subapical (Fig. 5VV)

Gonopore central

A. akazu Matsumura

A. fusina Yang & Zhang

22

A. badjawae Dworakowska

23

A. falcata Yang & Zhang

24

A. ginlingensis Yang & Zhang
25

26

27

A. forcipiformis Yang & Zhang

A. subapicifixa Yang & Zhang

A. rolikae Dworakowska

29

A. sufflata Yang & Zhang
29
A. wolongensis Yang & Zhang

A. maculosa Dworakowska

10 Tan W et al

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Aedeagus of Anufrievia spp, lateral view. A. A. adaucta Cao & Zhang, 2018; B. A. arcuata
Yang & Zhang, 2018; C. Anufrievia badjawae Dworakowska, 1976; D. Anufrievia bauhinicola
Dworakowska & Viraktamath, 1978; E. A. curva Yang & Zhang, 2018; F. A. expansa Cao &
Zhang, 2018; G. A. falcata Yang & Zhang, 2018; H. A. forcipiformis Yang & Zhang, 2018; I. A.
fusina Yang & Zhang, 2018; J. A. Jinghongensis Cao & Zhang, 2018; K. A. liubanus Yang &
Zhang, 2018; L. A. parisakazu Cao & Zhang, 2018; M. A. plana Yang & Zhang, 2018; N. A.
qinlingensis Yang & Zhang, 2018; O. A. quadrata Cao & Zhang, 2018; P. A. sohenoides Yang
& Zhang, 2018; Q. A. subapicifixa Yang & Zhang, 2018; R. A. subdentata Yang & Zhang,
2018; S. A. sufflata Yang & Zhang, 2018; T. A. symmetrica Cao & Zhang, 2018; U. A.
triangulata Cao & Zhang, 2018; V. A. triprocessa Yang & Zhang, 2018; W. A. wolongensis
Yang & Zhang, 2018; X. Anufrievia zelta Dworakowska, 1977.

Two new species of the genus Anufrievia Dworakowska from China (Hemiptera: ... 11

Acknowledgements

This study was partly funded by the World Top Discipline Program of Guizhou Province:
Karst Ecoenvironment Sciences (No.125 2019 Qianjiao Keyan Fa), the Guizhou Provincial
Science and Technology Foundation ((2018]1411), the Guizhou Science and Technology
Support Project ([2019]2855), the Science and Technology Project of Guiyang City
([2020]7-18), the Innovation Group Project of Education Department of Guizhou Province
([2021]013), the Training Program for High-level Innovative Talents of Guizhou Province
([2016]4020) and the Project for Regional Top Discipline Construction of Guizhou Province:
Ecology in Guiyang University [Qian Jiao Keyan Fa [2017]85].

References

° Cao YH, Yang MX, Lin SH, Zhang YL (2018) Review of the leafhopper genus Anufrievia
Dworakowska (Hemiptera: Cicadellidae: Typhlocybinae: Erythroneurini). Zootaxa 4446
(2): 203-232. https://doi.org/10.11646/zootaxa.4446.2.2

° Dietrich CH (2005) Keys to the families of Cicadomorpha and subfamilies and tribes of
Cicadellidae (Hemiptera: Auchenorrhyncha). Florida Entomologist 88: 502-517.
https://doi.org/10.1653/0015-4040(2005)88[502:KT TFOC]2.0.CO;2

° Dworakowska | (1970) On the genera Asianidia Zachv. and Singapora Mahm. with the
description of two new genera (Auchenorrhyncha, Cicadellidae, Typhlocybinae). Bulletin
de l’Academie Polonaise des Sciences 18 (12): 759-765.

° Dworakowska | (1993) Remarks on Alebra Fieb. and Eastern Hemisphere Alerini
(Auchenorrhyncha: Cicadellidae: Typhlocybinae). Entomotaxonomia 15 (2): 91-121.
° Matsumura S (1932) A revision of the Palaearctic and Oriental typhlocybid-genera with

descriptions of new species and new genera. Insecta Matsumurana 6 (3): 93-120.