Biodiversity Data Journal 11: e104877 CO) doi: 10.3897/BDJ.11.e104877 open access Taxonomy & Inventories First record of the genus Pectapalochrus Tshernyshev, 2016 (Coleoptera, Malachiidae) from China Junbo Tong?, Sergei E. TshernyshevS!, Haoyu Liu*, Yuxia Yang* + The Key Laboratory of Zoological Systematics and Application, School of Life Science, Institute of Life Science and Green Development, Hebei University, 071002, Baoding, China § Institute of Systematics and Ecology of Animals, Siberian Branch of the Russian Academy of Sciences, Frunze Street 11, 630091, Novosibirsk, Russia | Tomsk State University, Lenina prospekt 36, 634050, Tomsk, Russia Corresponding author: Haoyu Liu (liuhy@hbu.edu.cn), Yuxia Yang (yxyang@hbu.edu.cn) Academic editor: Enrico Ruzzier Received: 12 Apr 2023 | Accepted: 25 Apr 2023 | Published: 10 May 2023 Citation: Tong J, Tshernyshev SE, Liu H, Yang Y (2023) First record of the genus Pectapalochrus Tshernyshev, 2016 (Coleoptera, Malachiidae) from China. Biodiversity Data Journal 11: €104877. https://doi.org/10.3897/BDJ.11.e104877 Abstract Background Pectapalochrus Tshernyshev, 2016 is a small genus belonging to the tribe Apalochrini in the family Malachiidae (Coleoptera, Cleroidea), with four currently known species: P omega (Evers, 1965) from Mongolia, P armenus (lablokoff-Khnzorian, 1978) from Armenia, P. pictus (Motschulsky, 1860) from Russia and P. pectinicornis (Erichson, 1840) from Russia and Mongolia. New information Pectapalochrus Tshernyshev, 2016 is newly recorded from China upon the discovery of P omega (Evers, 1965) from Ningxia Autonomous Region. The morphological characters of © Tong J et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 2 Tong J et al the adult are re-described in detail with illustrations of external appearance and relevant characters. Keywords new record, Apalochrini, taxonomy, China, Cleroidea Introduction The genus Pectapalochrus was proposed by Tshernyshev (2016a) on the basis of four species separated from Dromanthomorphus Pic, 1921 of Malachiidae (Majer 2002, Mayor 2007, Bocakova et al. 2012). Pectapalochrus was attributed to Apalochrini due to its long and pectinate antenna in male with the small pedicel almost hidden by the scape and can be distinguished from Dromanthomorphus by its slender fore and middle legs and simple metaventrite and pygidium (apical tergite) without appendages or apophyses (Tshernyshev 2016a). In contrast, Dromanthomorphus has swollen fore and middle legs and modified metaventrite possessing apophysis directed forwards (Tshernyshev 2016b). Besides, the members of Pectapalochrus are all constricted to the Palaearctic Region (Tshernyshev 2016a), while Dromanthomorphus is in the Oriental Region (Tshernyshev 2016b). To date, the genus Pectapalochrus is comprised of four species distributed in Armenia, Mongolia and Russia (Tshernyshev 2016a). In the present study, some specimens of this genus were discovered from China and identified as P omega (Evers, 1965), which represents the first record of Pectapalochrus from the Chinese fauna. Pectapalochrus omega is the type species of the genus Pectapalochrus and shows an obvious elytral pattern variation, which is very rare in the family Malachiidae (Evers 1969, Tshernyshev 2016a). Hence, a more detailed description of the species, as well as illustrations of external appearance and special characters with a distribution map are provided. The female ovipositor, pygidium (apical tergite) and ultimate abdominal ventrite (apical sternite) of P omega are illustrated and described for the first time. Materials and methods In this study, Malachiidae beetles are considered as a family (Majer 1994, Majer 2002, Mayor 2007, Bocakova et al. 2012, Constantin 2021). A new system, based on a cladistic study of several species from different Cleroidea families, resulted in the Malachiidae being assigned to a subfamily of the Melyridae sensu lato (Gimmel et al. 2019). To build a stable system, further phylogenetic analysis with more representatives of this family including the determination of typical molecular characteristics is required. For descriptions, special male structures and genitalia were studied. The term “special male structures” is not analogous to the term “Excitatoren’”, that means different kinds of structures located in different parts of the male body of soft-winged flower beetles and bearing ducts of pheromone glands necessary for female attraction and successful First record of the genus Pectapalochrus Tshernyshev, 2016 (Coleoptera, ... 3 copulation (Evers 1956, Evers 1963, Evers 1988, Matthes 1962). The “special male structures” includes all typical parts of the male, irrespective of their having pheromone glands or not. Terminology of terminalia morphology is according to Lawrence et al. (2010), namely (in comparison with previously used terms): pygidium for apical tergite, ultimate abdominal ventrite for apical sternite and endophallus for the inner sac of the aedeagus. The specimens examined in this study are deposited in Museum of Hebei University, Baoding, China (MHBU). The specimen had its abdomen detached and soaked in 10% solution of sodium hydroxide (NaOH) by boiling for several minutes. Ovipositor was dyed with haematoxylin. Genitalia were dissected, cleaned and transferred to glycerol on slides and photographed with a LEICA DFC450 colour digital camera attached to the LEICA M205 A microscope. LAS V.4.7 software was used to capture genitalia images. External morphology was observed with the Nikon SMZ1500 stereomicroscope. Images of adults were taken with a Canon EOS 80D digital camera and stacked in Helicon Focus 7. The final plates were prepared in Adobe Photoshop CS 6.0. Taxon treatments Pectapalochrus Tshernyshev, 2016 Nomenclature Pectapalochrus Tshernyshev, 2016 - Tshernyshev 2016a: 349. Type species Apalochrus omega Evers, 1965 Diagnosis Body medium-sized, ranging from 2.8 to 4.7 mm in length. Antennae flabellate or strongly serrate, antennomere 2 very small and not conspicuous, antennomere 3 triangular (Fig. 2b). Tarsomere 2 of the fore tarsi bearing a special comb in male (Fig. 2 g). Ultimate abdominal ventrite (apical sternite) shortened and divided in both male and female (Fig. 2h, Fig. 3b). Distribution China (new faunistic record: Ningxia), Russia, Mongolia, Armenia. Tong J et al ~~ swan a 4 Figure 1. El Pectapalochrus omega (Evers, 1965), habitus: a male, dorsal view; b female, dorsal view. Scale bars: 1.0 mm. Figure 2. EES Main characters of Pectapalochrus omega (Evers, 1965), male. a head; b antenna; c labium; d maxilla; e labrum; f mandible; g fore tarsi; h ultimate abdominal ventrite (apical sternite); i pygidium (apical tergite); j male genitalia, lateral view, k male genitalia, ventral view. Scale bars: 0.2 mm. First record of the genus Pectapalochrus Tshernyshev, 2016 (Coleoptera, ... 5 Figure 3. EES Pectapalochrus omega (Evers, 1965), female: a pygidium (apical tergite); b ultimate abdominal ventrite (apical sternite); c ovipositor. Scale bars: 0.2 mm. Pectapalochrus omega (Evers, 1965) Nomenclature Apalochrus omega Evers, 1965 - Evers 1965: 149 (type locality: Mongolia). Flabellapalochrus omega (Evers, 1965): Evers, 1987 - Evers 1987: 59. Dromanthomorphus omega (Evers, 1965): Wittmer, 1990 - Wittmer 1990: 112; Mayor, 2007 - Mayor 2007: 416. Pectapalochrus omega (Evers, 1965): Tshernyshev, 2016 - Tshernyshev 2016a: 351. Apalochrus boops Evers, 1968 - Evers 1968: 33. Synonymized by Evers 1969: 188. Materials a. country: China; stateProvince: Ningxia; county: Zhongwei; municipality: Shapotou; year: 1987; month: 6; day: 8; individualCount: 3; sex: 1 male, 2 females; lifeStage: adult; recordedBy: Guodong Ren; institution|D: Museum of Hebei University; institutionCode: MHBU; occurrence!lD: FC88DCF7-9E42-5A67-8D3C-E8A8250B640F b. country: China; stateProvince: Ningxia; county: Zhongwei; locality: Gantang, Shapotou; year: 1987; month: 6; individualCount: 1; sex: 1 male; lifeStage: adult; recordedBy: Guodong Ren; institution|D: Museum of Hebei University; institutionCode: MHBU; occurrence!|D: ECC90ED4-65B2-5B5E-8A71-FA2142F862B8 Cc. country: China; stateProvince: Ningxia; county: Yanchi; locality: Qingyangjing; verbatimElevation: 1469; verbatimLatitude: 37.96°N; verbatimLongitude: 107.18°E; year: 2017; month: 5; day: 19; individualCount: 2; sex: 2 female; lifeStage: adult; recordedBy: Kang Lou; institution|D: Museum of Hebei University; institutionCode: MHBU; occurrence!D: F444DB70-F6B2-5201-ACEA-6C3381451A88 Tong J et al d. country: China; stateProvince: Ningxia; county: Yanchi; locality: Xijingtan; verbatimElevation: 1343; verbatimLatitude: 37.87°N; verbatimLongitude: 107.57°E; year: 2017; month: 5; day: 27; individualCount: 1; sex: 1 female; lifeStage: adult; recordedBy: Kang Lou; institution|D: Museum of Hebei University; institutionCode: MHBU; occurrence!D: 5CE8568D-3B16-5F 49-8558-C1810667F443 e. country: China; stateProvince: Ningxia; county: Yanchi; locality: ZhouzhuangzZi; verbatimElevation: 1452; verbatimLatitude: 37.74°N; verbatimLongitude: 107.36°E; year: 2017; month: 6; day: 10; individualCount: 1; sex: 1 male; lifeStage: adult; recordedBy: Kang Lou; institution!D: Museum of Hebei University; institutionCode: MHBU; occurrence!D: 2625194C-EB6B-5C7A-BE4F-B1021003ED0C Description Male. Length of body 3.5—4.7 mm, width at widest part of elytra 1.5—-2.3 mm and at the base of elytra 1.2-1.6 mm. Head capsule black. Antennae yellow to black: antennomere 1 yellow with a dark spot on inner side; 2 entirely yellow; 3-8 yellow to black; 9-11 entirely black. Mouthparts black. Pronotum black. Elytron with variable black and yellow markings, from black with a small yellow spot near apex to yellow with two black stripes at humerus and apical third. Scutellar shield black. Legs yellow to black (Fig. 1a). Ventral surface black. Vesicles yellow. Dorsum with double pubescence consisting of adpressed pubescence and sparse white stiff bristles. Sculptures evenly punctuated, stronger on elytra than on other parts. Head almost as wide as pronotum (Fig. 1a). Frons flat, interocular area not depressed and lacking protuberances (Fig. 2a). Antennae long and flabellate, projecting beyond middle of elytra; antennomere 1 widened and club-shaped; 2 small and almost completely hidden by 1; 3 enlarged, right triangular; 4-10 serrate to flabellate and almost equal in length; 11 elongate and slender (Fig. 2b). Labial palps with 3 palpomeres, apical palpomere subcylindrical (Fig. 2c). Maxilla with cardo short; palpomere 2 triangular; palpomere 3 small; palpomere 4 cylindrical and robust (Fig. 2 d). Clypeus distinct and membranous (Fig. 2e). Mandible robust, with a subapical tooth just behind apical tooth (Fig. 2f). Pronotum subrounded, lateral sides evenly rounded, anterior margin slightly convex, posterior margin straight, with a distinct transverse depression at base (Fig. 1a). Scutellar shield small and transverse, with smoothed edges (Fig. 1a). Elytra subparallel, widened behind the base and evenly rounded at apices, base of elytra distinctly wider than pronotum. Humeri distinct, slightly protruding. Elytral apices evenly rounded (Fig. 1a). Hind wings normally developed. Legs slender. Hind femora not reaching elytral apices. All tibiae thin and straight (Fig. 1 a). All tarsi with 5 tarsomeres; tarsomere 2 of the anterior tarsi with comb extending First record of the genus Pectapalochrus Tshernyshev, 2016 (Coleoptera, ... 7 over the segment; tarsomere 5 longest and tarsomere 4 shortest in all legs. Claws long and sharp, with small rounded bases (Fig. 2g). Metathorax simple, lacking appendages. Pygidium transverse, with almost straight distal side (Fig. 2i). Ultimate abdominal ventrite bilobed, short and transverse (Fig. 2h). Tegmen elongate; aedeagus wide, approximately parallel-sided for basal 2/3 in ventral view, then slightly narrowing to blunt apex; endophallus with two rows of small spines (Fig. 2j—-k). Female. Length of body 3.5—4.6 mm, width at widest part of elytra 1.6—2.2 mm and at the base of elytra 1.1-1.6 mm. Similar to male species, except for antennae short and serrate, fore-tarsi lacking comb, pronotum with an inconspicuous transverse depression at base (Fig. 1b). Pygidium sub-trapezoid with apical margin almost straight (Fig. 3a). Ultimate abdominal ventrite divided, with long spiculum ventrale (Fig. 3b). Ovipositor elongate and membranous (Fig. 3c). Diagnosis This species is similar to P. pectinicornis (Erichson, 1840) in entirely black pronotum and yellow to black antennae and legs, but can be distinguished from the latter by the elytra with variable black and yellow markings, antennomere 3 scalene triangular in male and subcylindrical in female (Fig. 1, Fig. 2b). In P. pectinicornis, elytra are black with wide yellow rounded spots at apices, antennomere 3 is equicrural triangular in male and triangular in female (Tshernyshev 2016a). Distribution China (Ningxia); Mongolia (Fig. 4). Figure 4. EE Distribution map of Pectapalochrus omega (Evers, 1965) in Mongolia and Ningxia Autonomous Region of China (red circle). 8 Tong J et al Acknowledgements The present study was financially supported by the National Natural Science Foundation of China (No. 32270491), the Biodiversity Survey and Assessment Project of the Ministry of Ecology and Environment, China (2019HJ2096001006) and the Youth Scientific Research and Innovation Team of Hebei University (605020521005). Additionally, this study was supported in part by the Program of Basic Scientific Research (FNI) of the State Academies of Sciences (No. 0247-2021-0004). References ° Bocakova M, Constantin R, Bocak L (2012) Molecular phylogenetics of the melyrid lineage (Coleoptera: Cleroidea). Cladistics 28 (2): 117-129. https://doi.org/10.1111/j. 1096-0031.2011.00368.x ° Constantin R (2021) Malachiidae Fleming, 1821. In: Tronquet M (Ed.) Catalogue des Coléoptéres de France. 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