Biodiversity Data Journal 11: e101464 OO) doi: 10.3897/BDJ.11.e101464 open access Data Paper Alien and native species in Italian marine and transitional waters Cristina Di Muri*$, Tamara Lazicl, Ilaria Rosati+, Cataldo Pierri, Angela Boggero*, Giuseppe Corriero!, Alberto Basset™’S + National Research Council (CNR), Research Institute on Terrestrial Ecosystems (IRET), Lecce, Italy § LifeWatch ERIC, Lecce, Italy | University of Bari, Bari, Italy q| LifeWatch Italy, Lecce, Italy # National Research Council (CNR), Water Research Institute (IRSA), Verbania, Italy = University of Salento, Lecce, Italy Corresponding author: Cristina Di Muri (cristina.dimuri@iret.cnr.it), Tamara Lazic (tamara.lazic@uniba..it) Academic editor: Christos Arvanitidis Received: 03 Feb 2023 | Accepted: 21 Apr 2023 | Published: 27 Apr 2023 Citation: Di Muri C, Lazic T, Rosati |, Pierri C, Boggero A, Corriero G, Basset A (2023) Alien and native species in Italian marine and transitional waters. Biodiversity Data Journal 11: e101464. https://doi.org/10.3897/BDJ.11.e101464 Abstract Background Biological invasions are one of the major threats to the ecosystem structure and functioning. After the initial introduction, frequently mediated by human activities, alien species can overcome different biogeographical and ecological barriers and determine severe impacts on native biodiversity and socio-economic activities. The Italian peninsula is located at the intersection of large trade routes within the Mediterranean Sea. Such position, along with the intense commercial activity and the high population density of the Italian coast, are considered important drivers of alien species in Italian marine and transitional ecosystems. The Italian peninsula, however, is also one of the regions with the highest native species richness within the Mediterranean Sea and, therefore, it is crucial to account for both alien and native species diversity when estimating the impact of biological invasion. Yet, such comprehensive information is frequently scattered across several biodiversity information systems and databases. © Di Muri C et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 2 Di Muri C et al New information Here, two datasets with alien and native species records in Italian marine and transitional waters are described. These datasets, created for the LifeWatch Italy case study on alien species, are the result of a large-scale collaboration involving experts working across the whole range of taxonomic diversity. The marine dataset includes a total of 12,219 records belonging to 3,772 species gathered from 91 investigated sites and seven EUNIS habitats. The dataset on transitional waters biodiversity includes 3,838 records belonging to 2,019 species found in 23 locations and four EUNIS habitats. Alien species were recorded in both marine and transitional waters, accounting respectively for 140 and 171 biological records belonging to 59 and 97 species. These occurrence data can be used for further research studies or management purposes, including the evaluation of the invasion risk and the formulation of alien species control and management plans. Furthermore, these compiled datasets can be used as input data for the Biotope vulnerability case study of LifeWatch ERIC, which offers a number of ICT services for the calculation of the incidence and of the impact of alien species on European biotopes. Keywords non-indigenous species, introduced species, biodiversity, transitional ecosystems, marine ecosystems, EUNIS habitats, LifeWatch Introduction Biological invasions are a major driver of ecosystem changes (PySek et al. 2020) and the dispersal of alien and invasive species in aquatic ecosystems have already determined severe impacts upon ecosystems functions and biodiversity as well as on socio-economic activities (e.g. Katsanevakis et al. (2014), Morri et al. (2019)). The Mediterranean Sea is considered a hotspot of biological invasions (Bailey et al. 2020, Galil et al. 2018). Throughout time, the Mediterranean has experienced drastic biological changes, occasionally consisting in the replacement of most of its biota. These changes, however, have occurred naturally over thousands of years due, for example, to extreme variations in environmental conditions (Rilov and Galil 2009). In the anthropocene, human- mediated activities are determining comparable biodiversity changes that are taking place at a much faster pace and over shorter timescales (Giangrande et al. 2020, Rilov and Galil 2009). The opening of the Suez Canal in 1869 has facilitated the arrival of new species and climate change is fostering further aided and unaided migrations in the Mediterraneanean which, over the last five decades, has experienced the highest rate of biological introductions (Galil et al. 2018, Mannino et al. 2017, Zenetos et al. 2017). In the Mediterranean Sea, coastal areas and, particularly, transitional water ecosystems such as estuaries, lagoons and coastal lakes, are largely exploited for their resources as well as for fisheries and acquaculture activities (Perez-Ruzafa and Marcos 2012). The Alien and native species in Italian marine and transitional waters 3 anthropogenic pressure on such ecosystems has resulted in the degradation and loss of habitats, as well as in the extirpation of local biodiversity, which has been further threatened by the large number of bioinvasions (Lotze et al. 2006). Despite transitional water ecosystems covering only a small fraction of the Mediterranean's coastline, they provide more ecosystem services and societal benefits than other aquatic ecosystems and hold numerous endemisms and unique species assemblages (Chapman 2012, Elliott and Whitfield 2011). Transitional waters are, however, particularly susceptible to biological invasions as they offer natural refugees and favourable conditions for the arrival and settlement of new species (Occhipinti-Ambrogi et al. 2010). The Italian peninsula, with its 171 transitional water bodies spread along 7,000 km of coastline (Cardone et al. 2013, Servello et al. 2019), lies in a strategic position within the Mediterranean Basin as it is located at the crossroads of three different subregions (Directive 2008/56/EC, European Parliament (2008)) and at the intersection of water masses with different hydrographic conditions (Corriero et al. 2015, Occhipinti-Ambrogi et al. 2010). Italy represents the main passageway of the maritime traffic within the Mediterranean and such pathway is responsible for half of the total alien species introductions along the Italian coast (Servello et al. 2019). Notwithstanding their high invasibility, Italian coastal and transitional waters also harbour a rich native biological diversity (Bianchi and Morri 2000). Native species diversity and community composition are considered key factors in the ecosystem responses to biological invasions; hence, it is crucial to take these ecosystem components into account when studying the impact of biological invasions within a certain geographic area or habitat (Corriero et al. 2015, Colangelo et al. 2017). To date, a number of studies focused on the collection and publication of alien and invasive species checklists along the Italian coast (Occhipinti- Ambrogi et al. 2010, Servello et al. 2019). However, there is a lack of geo-referenced datasets reporting comprehensive biodiversity information including records of native and alien species across a broad range of taxonomic groups and locations in Italian marine and transitional waters. The access to accurate and comprehensive species distribution data is crucial to evaluate spatio-temporal changes in biodiversity patterns, as well as the impact of alien species. Detailed information of species occurrence can improve our ability to quickly react and mitigate the challenge of biological invasions. Biodiversity data, however, are usually spread over a number of different platforms (e.g. GBIF, EMODnet, OBIS) and it is often difficult for scientists and stakeholders to access such an enormous amount of heterogeneous and diverse information. Furthermore, several international open-access databases, such as the World Register of Marine Species, FishBase and AlgaeBase, gather biodiversity information focusing on a single taxonomic group or ecosystem and it can be difficult to simultaneously extract and combine data from different sources into unique and standardised datasets for different taxa and locations of interest. Here, two datasets with geo-referenced occurrence records in Italian marine and transitional waters are described. The presented datasets include comprehensive biodiversity information belonging to different taxonomic groups (i.e. phytoplankton, algae, invertebrates and fish) and cover several habitats and regions all along the Italian peninsula. In both datasets, 4 Di Muri C et al each record is associated with the status information, i.e. if the taxon is considered alien or native in the geographic area in which it occurs. Moreover, for each geo-referenced occurrence, the EUNIS habitat type is defined. General description Purpose: In this paper, two datasets with occurrence records of native and alien species in Italian marine and transitional ecosystems are presented. These datasets are two research products generated within the LifeVVatch Italy case study on alien species. The coordinatio n_ team of this case study, with the collaboration of a larger interest group, developed between 2013 and 2015, a series of resources and facilities for the research community engaged with alien species and available through the LifeVVatch Italy website. The research facilities include a virtual research environment, a thesaurus and five biodiversity datasets with native and alien species occurrences. Altogether these resources contribute to an enhanced access and (re)use of scientific information on alien and native biodiversity on the Italian peninsula. Here, the marine and the transitional waters biodiversity datasets are described in detail. The marine dataset includes more than 12,200 records distributed over 91 sites and seven EUNIS habitats (level 2) and the transitional waters dataset holds over 3,800 records gathered from 23 sites and four EUNIS habitats (levels 2 and 3). These occurrence collections provide accurate and verified distribution information that could be used by the scientific community in further research studies and by stakeholders and policy-makers to define conservation priorities, to improve monitoring programmes and to enforce the current regulation with the aim of mitigating the risk of new invasions and of controlling the spread of established alien species. Additionally, these datasets can be used as input files for the Biotope vulnerability workflow developed within the Internal Joint Initiative of LifeWatch ERIC. Such analytical workflow uses native and alien species occurrences to estimate the incidence and the impact of alien species on different European habitats. Project description Study area description: Marine and transitional areas of the Italian peninsula (Fig. 1). The occurrence records included in the datasets are widely distributed along the coast of Italy, encompassing the Ligurian, Tyrrhenian, lonian and Adriatic Seas and spanning from Friuli Venezia Giulia to Sicily (northernmost to southernmost locations) and from Sardinia to Apulia (westernmost to easternmost locations). However, some geographical areas are more covered than others (Fig. 1) as occurs in most national and international biodiversity datasets. The uneven distribution of biodiversity records is inevitably associated with a better biological and ecological understanding of some biogeographical areas at both national and global levels. Our comprehensive collection of biological information can, therefore, also be used to inform environmental managers and stakeholders that more intense biomonitoring efforts should be directed towards those areas that are currently understudied. Alien and native species in Italian marine and transitional waters 3 Latitude (DD) Longitude (DD) + Marine O Transitional waters Figure 1. EESI Map of the marine (green crosses) and transitional (blue circles) locations included in the datasets. Design description: Occurrence records were gathered from different research institutions and consortia associated with LifeWatch Italy (Table 1) and have been used in published studies (check, for example, Boggero et al. (2014), Corriero et al. (2015)) to assess the habitat vulnerability to alien species within the framework of the LifeWatch Italy Alien Species use case. In both datasets, each record is associated with taxonomic information (i.e. Species, genus, family, order, class, phylum, kingdom) and sampling event details including date, location and geographical coordinates. Furthermore, the EUNIS classification system was used to define the type of habitat and species group associated with each record and an additional column was used to specify whether the species is considered alien in the corresponding location of the Italian coast. The adopted definition of alien species is available in the Alien Species Thesaurus produced by LifeWatch Italy, according to which, a species is considered alien if it is deliberately or inadvertently introduced by human activities outside its past or current distribution (Boggero et al. 2014). 6 Di Muri C et al Table 1. List of LifeWatch Italy institutions involved in the biodiversity data collections for the two published datasets. LifeWatch Italy institutions Apulian Regional Agency for the Environmental Prevention and Protection (ARPA) Centre for Estuarine and coastal MArine Sciences (CEMAS) of Venice National Institute of Oceanography and Experimental Geophysics (OGS) of Trieste National Research Council (CNR), Institute for Coastal Marine Environment (IAMC) of Taranto and Castellammare del Golfo now Water Research Institute (IRSA) National Research Council (CNR), Institute of Marine Science (ISMAR) of Venice National Research Council (CNR), Water Research Institute (IRSA) of Verbania Polytechnic University of Marche Sapienza University of Rome University of Bari "Aldo Moro" University of Ferrara University of Genoa University of Perugia University of Salento University of Sassari Zoological Station "Anton Dohrn" of Naples Sampling methods Description: The occurrence records included in the marine dataset were collected from 1985 to 2015, whereas the records included in the dataset on transitional ecosystems are from 1940 to 2015. Sampling description: Taxonomic records were gathered from datasets belonging to several public and private research institutions (Table 1). Quality control: After data collection, all records were standardised and validated. Different platforms were queried to check for taxonomic reliability and taxonomic consistency, including the taxa-match tools available in the Pan-European Species directories Infrastructure (PESI), World Register of Marine Species (WoRMS) and Catalogu e of Life (CoL). Specifically, PESI was initially used for the taxonomic matches, when a match was not found, WoRMS was queried and, in case WoRMS was not responsive, CoL was used at last. Datasets’ curation was then followed by a final check carried out by Alien and native species in Italian marine and transitional waters rs Italian taxonomic experts of each specific taxonomic group (i.e. phytoplankton, fish, algae, invertebrates). The taxonomic experts contributed to the validation of each record by: 1. evaluating if the reported occurrence for geographic area was reliable and 2. assigning the status of the taxon (alien vs. native) in that area. Geographic coverage Description: The marine dataset includes biological records gathered from 91 sites (Bounding coordinates 45.70080 N, 36.900000 S, 18.493927 E, 7.89840 W), whereas biological records of the transitional waters dataset were collected across 23 sites (Bounding coordinates 46.9818 N, 36.900000 S, 18.493927 E, 7.3920000 VW). All locations within the datasets were classified according to the European Nature Information System (EUNIS) and considering the second and third level of the classification structure (Table 2). According to such classification system, the highest number of records for the marine dataset was found in the pelagic water column (EUNIS habitat code A7; Fig. 2a), followed by the circa-littoral rock and other hard substrata (EUNIS habitat code A4; Fig. 2a) and the infra-littoral rock and other hard substrata (EUNIS habitat code A3; Fig. 2a). The highest number of marine species was recorded in the circa-littoral rock and other hard substrata (EUNIS habitat code A4; Fig. 2b), followed by the pelagic water column (EUNIS habitat code A7; Fig. 2b) and the infra-littoral rock and other hard substrata (EUNIS habitat code A3; Fig. 2b). As for the transitional waters dataset, the majority of biological records and species occurred in coastal brackish habitats (EUNIS habitat code X03; Fig. 2) as opposed to saline coastal lagoons (EUNIS habitat code X02; Fig. 2). Coordinates: 36.519702 and 45.981695 Latitude; 6.679688 and 18.632813 Longitude. a b 5000 4000 3000 2000 Number of records Number of species 1000 Ai A2 A3 A4 AS AB AZ X02 X03 J5.1 J5.3 Ail A2 A3 A4 AS AGB AZ XO2 X03 J5.1 J5.3 EUNIS habitat type code Figure 2. EES] Barplots showing the number of records (a) and the number of species (b) per EUNIS habitat type included in the marine (green bars) and transitional waters (blue bars) datasets. 8 Di Muri C et al Table 2. List of EUNIS habitats included in the datasets with biological records in Italian marine and transitional waters. EUNIS CODE LEVEL HABITAT TYPE NAME DATASET Al 2 Littoral rock and other hard substrata Marine A2 2 Littoral sediment Marine A3 2 Infra-littoral rock and other hard substrata Marine A4 2 Circa-littoral rock and other hard substrata Marine AS 2 Sublittoral sediment Marine A6 2 Deep-sea bed Marine A7 2 Pelagic water column Marine X02 2 Saline coastal lagoons Transitional X03 2 Brackish coastal lagoons Transitional J5.1 3 Highly artificial saline and brackish standing waters Transitional J5.3 3 Highly artificial non-saline standing waters Transitional Taxonomic coverage Description: The marine dataset contains 12,219 occurrence records belonging to 3,772 species (3,715 native and 59 alien species) and 16 phyla (Fig. 3a, b). The highest number of both records and species was recorded in the phylum Mollusca, followed by Ochrophyta, Arthropoda, Annelida and Rhodophyta (Fig. 3a, b). The transitional waters dataset contains 3,838 occurrence records belonging to 2,019 species (1,925 native and 97 alien species) and 13 phyla (Fig. 3c, d). The highest number of records was found in the phylum Mollusca, followed by Arthropods, Annelida, Ochrophyta and Chordata, while the highest number of species was recorded in the phylum Arthropoda, followed by Mollusca, Ochrophyta, Annelida and Rhodophyta (Fig. 3c, d). It should be noted that the difference in numbers between total species across all phyla and total alien and native species is due to the status of some species which are considered native in some localities and alien in others. Specifically, such species are Ebria tripartita and Monocorophium sextonae for the marine dataset and Acanthophora_ nayadiformis, Amphibalanus eburneus and Erythrocladia irregularis for the transitional waters dataset. Usage licence Usage licence: Creative Commons Public Domain Waiver (CC-Zero) Alien and native species in Italian marine and transitional waters SS) IP rights notes: This work is licensed under the Creative Commons Attribution 4.0 International Licence. To view a copy of this licence, visit http://creativecommons.org/ licenses/by/4.0/. a Ciliophora 69 Tracheophyta 63 c Echinodermata 51. ‘hinodermata 122 eer Ha meee 7s pace as a Others 99 ¢ 237 aw Bry: Chlori sie a jodermata 31 Cnidaria 4 | Myz020a 56 a b Tracheophyta 55 Echinodermata 44 d Cillophora s9—_ \ Others 38 Haptophyta 63 Total native Chlorophyta 90: % Figure 3. EES] > - Donut charts showing the number of records (a, c) and the number of species (b, d) per phylum included in the marine (green; a, b) and transitional waters (blue; c, d) datasets. The pie charts within the donut charts represent the total number of alien and native records/ species for each dataset. Data resources Data package title: Biodiversity data of Italian marine and transitional ecosystems gathered for the alien species study case of LifeWatch Italy. Alternative identifiers: Marine dataset: https://doi.org/10.48372/30d8844e-1 7 74-4c63-89 c4-4d858f88317d; Transitional waters dataset: https://doi.org/10.48372/98914415-765c-46 50-b272-b16793231e8a Number of data sets: 2 Data set name: Biodiversity data of Italian marine ecosystems gathered for the alien species study case of LifeWatch Italy. 10 Di Muri C et al Download URL: https://dataportal.lifewatchitaly.eu/view/urn% 3Auuid %3A9f5 7ac94-87 55-4991-b1b4-fe4e871 7ec3d Data format: CSV Description: The dataset contains over 12,200 alien and native species occurrence records distributed across 91 marine sites and seven EUNIS habitats (level 2) along the Italian coast. Column label catalognumber eventdate waterbody locality decimallatitude decimallongitude phylum class order family genus providedscientificname scientificname scientificnameauthorship eunishabitatstypecode alien Column description An identifier (preferably unique) for the record within the data set or collection. The date-time or interval during which an Event occurred. For occurrences, this is the date-time when the event was recorded. The name of the water body in which the Location occurs. The specific description of the place. This term may contain information modified from the original to correct perceived errors or standardize the description. The geographic latitude of the geographic center of a Location (in decimal degrees, using the spatial reference system WGS84). Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The geographic longitude of the geographic center of a Location (in decimal degrees, using the spatial reference system WGS84). Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The full scientific name of the phylum or division in which the taxon is classified. The full scientific name of the class in which the taxon is classified. The full scientific name of the order in which the taxon is classified. The full scientific name of the family in which the taxon is classified. The full scientific name of the genus in which the taxon is classified. The scientific name, with authorship and date information if known, as it originally appeared before of the taxonomic check. The full scientific name, with authorship and date information if known. When forming part of an Identification, this should be the name in lowest level taxonomic rank that can be determined. This term should not contain identification qualifications, which should instead be supplied in the IdentificationQualifier term. The authorship information for the scientificName formatted according to the conventions of the applicable nomenclaturalCode. Assignment of the habitat type code based on the EUNIS habitat classification. A species, subspecies or lower taxon, introduced outside its natural past or present distribution; includes any part, gametes, seeds, eggs, or propagules of such species that might survive and subsequently reproduce. Alien and native species in Italian marine and transitional waters 11 eunisspeciesgroups namepublishedinyear Assignment of the organism group based on the EUNIS species groups. The four-digit year in which the scientificName was published. Data set name: Biodiversity data of Italian transitional ecosystems gathered for the alien species study case of LifeWatch Italy. Download URL: https://dataportal.lifewatchitaly.eu/view/urn%3Auuid%3Ab5e33b56-75 2c-4fe7-9a53-face2497e40c Data format: CSV Description: The dataset contains over 3,800 alien and native species occurrence records distributed across 23 transitional waters sites and four EUNIS habitats (levels 2 and 3) along the Italian coast. Column label catalognumber eventdate locality decimallatitude decimallongitude kingdom phylum class order family genus providedscientificname scientificname Column description An identifier (preferably unique) for the record within the data set or collection. The date-time or interval during which an Event occurred. For occurrences, this is the date-time when the event was recorded. The specific description of the place. This term may contain information modified from the original to correct perceived errors or standardize the description. The geographic latitude of the geographic center of a Location (in decimal degrees, using the spatial reference system WGS84). Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The geographic longitude of the geographic center of a Location (in decimal degrees, using the spatial reference system WGS84). Positive values are north of the Equator, negative values are south of it. Legal values lie between -90 and 90, inclusive. The full scientific name of the kingdom in which the taxon is classified. The full scientific name of the phylum or division in which the taxon is classified. The full scientific name of the class in which the taxon is classified. The full scientific name of the order in which the taxon is classified. The full scientific name of the family in which the taxon is classified. The full scientific name of the genus in which the taxon is classified. The scientific name, with authorship and date information if known, as it originally appeared before of the taxonomic check. The full scientific name, with authorship and date information if known. When forming part of an Identification, this should be the name in lowest level taxonomic rank that can be determined. This term should not contain identification qualifications, which should instead be supplied in the IdentificationQualifier term. 12 Di Muri C et al scientificnameauthorship The authorship information for the scientificName formatted according to the conventions of the applicable nomenclaturalCode. eunishabitatstypecode § Assignment of the habitat type code based on the EUNIS habitat classification. alien A species, subspecies or lower taxon, introduced outside its natural past or present distribution; includes any part, gametes, seeds, eggs, or propagules of such species that might survive and subsequently reproduce. eunisspeciesgroups Assignment of the organism group based on the EUNIS species groups. namepublishedinyear The four-digit year in which the scientificName was published. Acknowledgements The Authors would like to express their sincere gratitude to all the researchers who contributed to data collection and supported this work: Stefano Accoroni, Giorgio Alabiso, Serena Arima, Enrico Barbone, Mauro Bastianini, Giorgio Bavestrello, Anna Maria Bazzoni, Fabrizio Bernardi Aubry, Ferdinando Boero, Maria Cristina Buia, Marina Cabrini, Elisa Camatti, Frine Cardone, Bruno Cataletto, Riccardo Cattaneo Vietti, Francesco Cavraro, Ester Cecere, Tamara Cibic, Paolo Colangelo, Francesco Cozzoli, Alessandra De Olazabal, Gianfranco D'Onghia, Stefania Finotto, Nicola Fiore, Daniela Fornasaro, Piero Franzoi, Simonetta Fraschetti, Maria Cristina Gambi, Lorenzo Gaudiano, Adriana Giangrande, Vojsava Gjoni, Cinzia Gravili, Rosanna Guglielmo, Giovanna Jona-Lasinio, Erica Keppel, Caterina Longo, Maurizio Lorenti, Alessandro Ludovisi, Antonella Luglie, Porzia Maiorano, Stefano Malavasi, Francesco Mastrototaro, Maria Grazia Mazzocchi, Maria Mercurio, Michele Mistri, Marina Monti, Cristina Munari, Luigi Musco, Carlotta Nonnis Marzano, Bachisio Mario Padedda, Francesco Paolo Patti, Antonella Petrocelli, Maurizio Pinna, Stefano Piraino, Alessio Pollice, Giuseppe Portacci, Alessandra Pugnetti, Silvia Pulina, Tiziana Romagnoli, Leonilde Roselli, Diana Sarno, Cecilia Teodora Satta, Stefano Schiaparelli, Beatrice Scipione, Nicola Sechi, Adriano Sfriso, Marco Sigovini, Letizia Sion, Rocco Sorino, Elena Stanca, Davide Tagliapietra, Antonio Terlizzi, Valentina Tirelli, Cecilia Totti, Angelo Tursi, Nicola Ungaro, Adriana Zingone, Matteo Zucchetta and Valerio Zupo. Author contributions CDM and TL wrote the first draft of the manuscript. CDM produced the graphical representations and revised the manuscript and the datasets following the constructive feedback received from the Authors and from the initial technical evaluation of the journal. IR, CP, ABo, GC and ABa contributed to data collection, data aggregation and data quality control and standardisation. All Authors actively contributed to the manuscript editing and quality check and approved the submission of the article. Alien and native species in Italian marine and transitional waters 13 References Bailey S, Brown L, Campbell M, Canning-Clode J, Carlton J, Castro N, Chainho P, Chan F, Creed J, Curd A, Darling J, Fofonoff P, Galil B, Hewitt C, Inglis G, Keith |], Mandrak N, Marchini A, McKenzie C, Occhipinti-Ambrogi A, Ojaveer H, Pires-Teixeira L, Robinson T, Ruiz G, Seaward K, Schwindt E, Son M, Therriault T, Zhan A (2020) Trends in the detection of aquatic non-indigenous species across global marine, estuarine and freshwater ecosystems: a 50-year perspective. Diversity and Distributions 26 (12): 1780-1797. https://doi.org/10.1111/ddi.13167 Bianchi CN, Morri C (2000) Marine biodiversity of the Mediterranean Sea: situation, problems and prospects for future research. Marine Pollution Bulletin 40 (5): 367-376. https://doi.org/10.1016/s0025-326x(00)00027-8 Boggero A, Basset A, Austoni M, Barbone E, Bartolozzi L, Bertani |, Campanaro A, Cattaneo A, Cianferoni F, Corriero G, Dorr AM, Elia AC, Ficetola GF, Kamburska L, La Porta G, Lauceri S, Ludovisi A, Gaino E, Goretti E, Lorenzoni M, Manca M, Marchetto A, Morabito G, Marzano FN, OggioniA, Pierri C, Riccardi N, Rossetti G, Ungaro N, Volta P, Zaupa S, Fontaneto D (2014) Weak effects of habitat type on susceptibility to invasive freshwater species: an Italian case study. Aquatic Conservation: Marine and Freshwater Ecosystems 24 (6): 841-852. https://doi.org/10.1002/agc.2450 Cardone F, Corriero G, Fianchini A, Gravina MF, Nonnis Marzano C (2013) Biodiversity of transitional waters: species composition and comparative analysis of hard bottom communities from the south-eastern Italian coast. Journal of the Marine Biological Association of the United Kingdom 94 (1): 25-34. https://doi.org/10.1017/s00253154 13001306 Chapman P (2012) Management of coastal lagoons under climate change. Estuarine, Coastal and Shelf Science 110: 32-35. https://doi.org/10. 1016/j.ecss.2012.01.010 Colangelo P, Fontaneto D, Marchetto A, Ludovisi A, Basset A, Bartolozzi L, Bertani |, Campanaro A, Cattaneo A, Cianferoni F, Corriero G, Ficetola GF, Nonnis-Marzano F, Pierri C, Rossetti G, Rosati 1, Boggero A (2017) Alien species in Italian freshwater ecosystems: a macroecological assessment of invasion drivers. Aquatic Invasions 12 (3): 299-309. https://doi.org/10.3391/ai.2017.12.3.04 Corriero G, Pierri C, Accoroni S, Alabiso G, Bavestrello G, Barbone E, Bastianini M, Bazzoni AM, Bernardi Aubry F, Boero F, Buia MC, Cabrini M, Camatti E, Cardone F, Cataletto B, Cattaneo Vietti R, Cecere E, Cibic T, Colangelo P, De Olazabal A, D'onghia G, Finotto S, Fiore N, Fornasaro D, Fraschetti S, Gambi MC, Giangrande A, Gravili C, Guglielmo R, Longo C, Lorenti M, Luglié A, Maiorano P, Mazzocchi MG, Mercurio M, Mastrototaro F, Mistri M, Monti M, Munari C, Musco L, Nonnis-Marzano C, Padedda BM, Patti FP, Petrocelli A, Piraino S, Portacci G, Pugnetti A, Pulina S, Romagnoli T, Rosati 1, Sarno D, Satta CT, Sechi N, Schiaparelli S, Scipione B, Sion L, TerlizziA, Tirelli V, Totti C, Tursi A, Ungaro N, Zingone A, Zupo V, Basset A (2015) Ecosystem vulnerability to alien and invasive species: a case study on marine habitats along the Italian coast. Aquatic Conservation: Marine and Freshwater Ecosystems 26 (2): 392-409. https://doi.org/10.1002/aqc.2550 Elliott M, Whitfield AK (2011) Challenging paradigms in estuarine ecology and management. Estuarine, Coastal and Shelf Science 94 (4): 306-314. htips://doi.org/ 10.1016/j.ecss.2011.06.016 14 Di Muri C et al European Parliament CotEU (2008) Directive 2008/56/EC of the European Parliament and of the Council of 17 June 2008 establishing a framework for community action in the field of marine environmental policy (Marine Strategy Framework Directive). European Union. URL: http://data.europa.eu/eli/dir/2008/56/oj Galil B, Marchini A, Occhipinti-Ambrogi A (2018) East is east and West is west? Management of marine bioinvasions in the Mediterranean Sea. Estuarine, Coastal and Shelf Science 201: 7-16. https://doi.org/10.1016/j.ecss.2015.12.021 Giangrande A, Pierri C, Del Pasqua M, Gravili C, Gambi MC, Gravina MF (2020) The Mediterranean in check: biological invasions in a changing sea. Marine Ecology 41 (2). https://doi.org/10.1111/maec.12583 Katsanevakis S, Coll M, Piroddi C, Steenbeek J, Ben Rais Lasram F, Zenetos A, Cardoso AC (2014) Invading the Mediterranean Sea: biodiversity patterns shaped by human activities. Frontiers in Marine Science 1 https://doi.org/10.3389/fmars.2014. 00032 Lotze H, Lenihan H, Bourque B, Bradbury R, Cooke R, Kay M, Kidwell S, Kirby M, Peterson C, Jackson JC (2006) Depletion, degradation, and recovery potential of estuaries and coastal seas. Science 312 (5781): 1806-1809. httos://doi.org/10.1126/ science.1128035 Mannino A, Balistreri P, Deidun A (2017) The marine biodiversity of the Mediterranean Sea in a changing climate: the impact of biological invasions. Mediterranean Identities - Environment, Society, Culture https://doi.org/10.5772/intechopen.69214 Morri C, Montefalcone M, Gatti G, Vassallo P, Paoli C, Bianchi CN (2019) An alien invader is the cause of homogenization in the recipient ecosystem: a simulation-like approach. Diversity 11 (9). https://doi.org/10.3390/d11090146 Occhipinti-Ambrogi A, Marchini A, Cantone G, Castelli A, Chimenz C, Cormaci M, Froglia C, Furnari G, Gambi MC, Giaccone G, Giangrande A, Gravili C, Mastrototaro F, Mazziotti C, Orsi-Relini L, Piraino S (2010) Alien species along the Italian coasts: an overview. Biological Invasions 13 (1): 215-237. https://doi.org/10.1007/s10530-010- 9803-y Pérez-Ruzafa A, Marcos C (2012) Fisheries in coastal lagoons: an assumed but poorly researched aspect of the ecology and functioning of coastal lagoons. Estuarine, Coastal and Shelf Science 110: 15-31. https://doi.org/10.1016/j.ecss.2012.05.025 PySek P, Hulme P, Simberloff D, Bacher S, Blackburn T, Carlton J, Dawson W, Essl F, Foxcroft L, Genovesi P, Jeschke J, Kuhn I, Liebhold A, Mandrak N, Meyerson L, Pauchard A, Pergl J, Roy H, Seebens H, Kleunen M, Vila M, Wingfield M, Richardson D (2020) Scientists' warning on invasive alien species. Biological Reviews 95 (6): 1511-1534. https://doi.org/10.1111/brv.12627 Rilov G, Galil B (2009) Marine bioinvasions in the Mediterranean Sea — history, distribution and ecology. Biological Invasions in Marine Ecosystems549-575. https://doi .org/10.1007/978-3-540-79236-9 31 Servello G, Andaloro F, Azzurro E, Castriota L, Catra M, Chiarore A, Crocetta F, D’Alessandro M, Denitto F, Froglia C, Gravili C, Langer M, Lo Brutto S, Mastrototaro F, Petrocelli A, Pipitone C, Piraino S, Relini G, Serio D, Xentidis NJ, Zenetos A (2019) Marine alien species in Italy: a contribution to the implementation of descriptor D2 of the marine strategy framework directive. Mediterranean Marine Science https://doi.org/10. 12681/mms.18711 Alien and native species in Italian marine and transitional waters Zenetos A, Cinar ME, Crocetta F, Golani D, Rosso A, Servello G, Shenkar N, Turon X, Verlaque M (2017) Uncertainties and validation of alien species catalogues: the Mediterranean as an example. Estuarine, Coastal and Shelf Science 191: 171-187. https://doi.org/10.1016/j.ecss.2017.03.031