Biodiversity Data Journal 11: e102828 OO) doi: 10.3897/BDJ.11.e102828 open access Taxonomy & Inventories A new species of Megastigmus (Hymenoptera, Megastigmidae) from China Xiaoxiao Chen*, Jingge Kuangt, Wenjing Tao*, Zhongping XiongS, Kangshan Mao+ + Key Laboratory for Bio-Resource and Eco-Environment of Ministry of Education & Sichuan Zoige Alpine Wetland Ecosystem National Observation and Research Station, College of Life Science, Sichuan University, Chengdu 610065, Sichuan, China § College of Forests, Southwest Forestry University, Kunming 650224, Yunnan, China Corresponding author: Zhongping Xiong (xzping 0@126.com) Academic editor: Miles Zhang Received: 01 Mar 2023 | Accepted: 06 Apr 2023 | Published: 11 Apr 2023 Citation: Chen X, Kuang J, Tao W, Xiong Z, Mao K (2023) A new species of Megastigmus (Hymenoptera, Megastigmidae) from China. Biodiversity Data Journal 11: e102828. https://doi.org/10.3897/BDJ.11.e102828 ZooBank: urn:lsid:zoobank.org:pub:833A0B85-221B-4C43-8ACF-05966C0284BB Abstract Background Most species of Megastigmus are considered important economic pests that grow in seeds, especially of conifers. Accurate identification of species is a crucial step for the biological research of parasitic pests and the further application of biological control. However, their large variety, small size, similar morphology and different growth and development stages have brought great challenges to taxonomic research. Traditional morphological identification often takes a long time and this requires us to seek a new method for rapid and accurate identification. Therefore, the better identification of Megastigmus urgently needs to be combined with molecular methods to help taxonomic development. New information Here, Megastigmus daduheensis sp. n. (Chalcidoidea: Megastigmidae) was identified, based on morphology and molecular markers, such as CO/ and Cytb. M. daduheensis sp. © Chen X et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 2 Chen X et al n. is distinct from other Known species of the same genus in the morphology. The results of the molecular phylogenetic tree, similarity alignment and genetic distance indicate that the CO/ and Cytb sequences of MV. daduheensis sp. n. are highly similar to M. sobinae and M. duclouxiana, but there are some genetic differences. The genetic distances of M™. daduheensis sp. nov. with M. duclouxiana and M. sabinae were 0.34 and 0.33 and the percentages of shared base pairs were 76.3% and 76.8%, respectively. Both morphological and molecular data classified IV. daduheensis sp. n. as a new species. The obtained CO/ and Cytb sequences of M. daduheensis sp. n. can be used as DNA barcodes, providing molecular data for rapid and accurate identification of this species in the future. Keywords morphology, DNA barcoding , phylogenetic relationships, genetic distance Introduction Megastigmidae comprises over 200 species currently classified into 12 valid genera which are generally considered to have a worldwide distribution and Megastigmus is the only cosmopolitan genus of the family (BOhmova et al. 2022). Megastigmus was described by Dalman (1820) as the subgenus of Jorymus Dalman, with Pteromalus bipunctatus Swederus, 1795 as its type species. Later, Wegastigmus was recorded as a valid genus by several scholars and Crosby (1913) designated its type species as P bipunctatus (Curtis 1829, Dalla 1898, Ashmead 1904). Megastigmus is a diverse group with 144 valid species described in the Universal Chalcidoidea Database (Noyes 2019). Of these, most species have been described from the Holarctic and Australasian Regions (Boucek 1988, Grissel 1999). So far, 18 species in Megastigmus have been reported from China. The species of Wegastigmus display a great variety of feeding strategies in which many species are seed feeders and others are mostly solely carnivorous (Roques and Skrzypczynska 2003, BOhmova et al. 2022). Their habit leads to a decrease in seed yield which seriously endangers the reproduction of trees. We need to quickly identify and control the species to reduce damage. However, their small size and similar appearance makes it difficult to identify. The identification of closely-related species can no longer be met by using morphological characters alone. Therefore, rapid and accurate identification of Megastigmus insects and monitoring of their distribution and growth are one of the main measures for the prevention and control of these insects. DNA barcoding is a method for species identification, based on a relatively short DNA gene fragment with sufficient variation and easy amplification (Folmer et al. 1994, Nuriye et al. 2021). Currently, sequence fragments of mitochondrial DNA (mtDNA: CO/, Cytb, CO//, 16S etc.) have been widely used in this method, which can quickly and accurately identify insects (Janzen et al. 2017). In mitochondrial DNA, molecular markers, such as cytochrome c oxidase subunit | (CO/) and Cytochrome b (Cytb), are commonly used to A new species of Megastigmus (Hymenoptera, Megastigmidae) from China 3 study intra-species, inter-species and even inter-family phylogenetic relationships. They have the characteristics of high evolutionary rate, obvious interspecific variation, but relative conservation within species, good versatility of primers and easy amplification, which can effectively solve some long-standing controversies in morphological taxonomy ( Meyer and Wilson 1990, Koch 2010, Cheng et al. 2015, Chen et al. 2020). Therefore, the morphological characteristics combined with two molecular markers (CO/ and Cytb) can more effectively solve the problem of classification and identification of Megastigmus. Materials and methods The infested cones of C. chengiana were collected from Ri’er Township (102°35' E, 30°59’ N), Xiaojin County, Aba Autonomous Prefecture of Tibetan and Qiang (Ngawa), Sichuan Province, China. The cones were kept in mesh bags, where the spawning adult insects were collected and immediately frozen in liquid nitrogen and preserved at -80°C until DNA extraction. All type specimens are deposited in the Natural History Museum of Sichuan University, Chengdu (NHMSV) with an accession number NHMSU-20201015-XJ. Terminology of morphological structures in this study mostly follows Roques and Skrzypczynska (2003), Doganlar and Hassan (2010) and Doganlar (2015). Terms for surface sculpture follow Steinmann and Zombori (1985). Observations were made using Olympus SZX16 and BX53 microscopes. Photographs of diagnostic characters of the new species were taken by a KEYENCE VHX-5000 digital microscope. Abbreviations of used morphological characters are: POL = posterior ocellus longest diameter, OOL = shortest distance between posterior ocellus and eye margin, dorsal view; OCL = shortest distance between posterior ocellus and occipitial carina, dorsal view; F1—F7 = funicular 1-7; C1—C3 = clava 1-3. We used Mitoz 3.5.0 software (Meng et al. 2019) to assemble two mitochondrial gene fragments (CO/ and Cytb) from genomic sequencing data of MV. daduheensis sp. n. which was performed with Illumina HiSeq 2000 at Novogene, Beijing, China. Subsequently, a total of 27 CO/ and Cytb gene sequences of Megastigmus species were found in the National Center for Biotechnology Information (NCBI). Hence, the phylogenetic analysis used genetic data from the above species and one outgroup of Jorymus geranii Walker, 1833 (Table 1). The sequences of different species were manually aligned with clustalW in MEGA 7.0 (Kumar et al. 2016). The trimmed alignment was used to estimate the phylogeny by Maximum Likelihood (ML) using IQ-TREE 2.0.4 and an ultrafast bootstrap approximation approach with 1000 replicates (Fu et al. 2014) was used to calculate bootstrap scores for each node (BP) (Minh et al. 2020). MEGA 7.0 software was used to calculate the interspecific genetic distance, based on p-distance and the percentage of base pairs are shared between the species (Kimura 1980, Kumar et al. 2016). 4 Chen X et al Table 1. List of the mitochondrial genes analysed in the present study. Family/ Genus Species GenBank No. (CO/) GenBank No. (Cytb) Megastigmus M. daduheensis sp. n. ON421529 ON478368 M. aculeatus KF531842.1 JQ756668.1 M. amicorum KF531835.1 AY898663.1 M. atedius KF531848.1 AY898691.1 M. atlanticus KF531836.1 AY898669.1 M. bipunctatus KF531837.1 AY898664.1 M. borriesi KF531852.1 AY898681.1 M. cryptomeriae KF531841.1 AY898699.1 M. dorsalis AY317240.1 KX980153.1 M. duclouxiana ON421527 ON478366 M. hoffmeyeri KF531845.1 AY898695.1 M. lasiocarpae KF531853.1 AY898671.1 M. milleri KJ535739.1 AY898672.1 M. pictus KF531847.1 AY898696.1 M. pinsapinis KF531855.1 AY898684.1 M. pinus KF531850.1 AY898673.1 M. rafni KF531846.1 AY898678.1 M. rosae KF531840.1 AY898701.1 M. sabinae ON421528 ON478367 M. schimitscheki KF531854.1 AY898686.1 M. spermotrophus KF531844.1 AY898694.1 M. stigmatizans JQ417143.1 AY898703.1 M. strobilobius MN122037.1 AY898697.1 M. suspectus KF531856.1 AY898688.1 M. synophri FJ026673.1 FJ026729.1 M. thyoides KF531851.1 AY898667.1 M. tsugae KF531849.1 AY898682.1 M. zebrinus MN165915.1 KC710378.1 Torymidae Torymus geranii HM574310.1 GU123595.1 A new species of Megastigmus (Hymenoptera, Megastigmidae) from China 5 Taxon treatment Megastigmus daduheensis, sp. n. ° ZooBank 06D89B26-3CC3-4E8A-979B-8C860627B609 Materials Holotype: a. scientificName: Megastigmus daduheensis; order: Hymenoptera; family: Megastigmidae; genus: Megastigmus; specificEpithet: daduheensis; country: China; stateProvince: Sichuan; locality: Aba Autonomous Prefecture of Tibetan and Qiang (Ngawa), Xiaojin County, Ri’er Township (102°35' E, 30°59' N); locationRemarks: label transliteration:"15 X 2020, coll. Wenjing Tao, Wentao Wang and Xue Li"; individualCount: 1; sex: female; lifeStage: adults; occurrenceDetails: CollectionCode: NHMSU-20201015-XJ; GenBank No: CO/ (QN421529) and Cytb (ON478368).; occurrencelD: C3C6985C-AF91-54BA- ACE7-7689077A8ED3 Paratype: a. scientificName: Megastigmus daduheensis; order: Hymenoptera; family: Megastigmidae; genus: Megastigmus; specificEpithet: daduheensis; country: China; stateProvince: Sichuan; locality: Aba Autonomous Prefecture of Tibetan and Qiang (Ngawa), Xiaojin County, Ri’er Township (102°35' E, 30°59' N); locationRemarks: label transliteration:"15 X 2020, coll. Wenjing Tao, Wentao Wang and Xue Li"; individualCount: 38; sex: female 36 male 2; lifeStage: adults; occurrence!|D: E65DFF05-F6EE-579F-9958-F BF8F77337EE Description Female. Length (body + ovipositor): 2.81 ~ 3.05 + 3.09 ~ 3.17 mm. Colour: body (Fig. 1A, H) alternate distribution of yellow, brown and black; head: vertex brown, face yellow, clypeus with apical half dark brown; head antennae light brown; eye reddish- brown; clypeus with apical half dark brown; pronotum brownish-yellow; mesoscutum black anteriorly to brownish-yellow posteriorly; scutellum dark yellow; metanotum light yellow; propodeum black (Fig. 1J); leg brownish-yellow; wing hyaline, vein and the enlarged part of stigmal vein dark brown, stigma light brown; setae of body pale, having black base; hind coxa black, femora and tibia brown; gaster dark brown; ovipositor dark brown. Head. Head about 1.6x as wide as long in dorsal view; whole face with pale, dense setae; frons with many obvious striated sculptures; ocellus arranged in a blunt triangle, POL about twice as long as OOL and OCL; several short setae between ocelli, the rest on other parts of head (Fig. 1B—E). Antennae moniliform, arising from the area below centre of face; scape almost 3.5x as long as width, pedicel about 0.4 of scape length, anellus about 0.25 of pedicel length; relative length/breadth (ratio) of funiculars (F1—F7) as follows: F1 57/29 (1.96), F2 84/38 (2.21), F3 97/39 (2.48), F4 83/46 (1.80), F5 74/47 (1.57), F6 79/45 (1.76), F7 62/45 (1.38); clava (C1—C3) with relative length/breadth (ratio)131/47 (2.79) (Fig. 1F). Chen X et al Figure 1. EES] Megastigmus daduheensis sp. n. A-H female holotype; A Body, lateral view; B Head, lateral view; C Head, ventral view; D Head, dorsal view; E Head, frontal view; F Antenna; G Fore wing; H Head and mesosoma, dorsal view; I Male, Body, lateral view. Mesosoma. Pronotum and mesoscutum with irregular sculpture and orientation of rugae, notauli brown and sparse setae scattered on both sides (Fig. 1H); axillae with oblique longitudinal ridge; scultellum with transverse costula and white setae on both sides; propodeum with oriaceous, callus with long white setae and irregular reticulate sculpture; callus densely covered with white long setae. Basal cell of fore wing with more than ten setae and the lower part of the basal cell closed by a row of setae; marginal vein shorter than postmarginal vein; stigma large and 2.2x as long as width, stigma vein short and 0.11 as long as stigma (Fig. 1G). Hind coxa with white long setae on both sides, femora and tibia densely covered with white setae (Fig. 1A). A new species of Megastigmus (Hymenoptera, Megastigmidae) from China 7 Metasoma. Gaster not compressed laterally, 1.21 mm long, with sparse bristles scattered with four black horizontal bands and the spacing between the horizontal bands decreasing successively (Fig. 1A). Ovipositor densely covered with brown long setae and 4.5x as long as hind-leg tibia. Male. Body black, 2.4 ~ 2.6 mm long (Fig. 1l). Gaster black, 1.0 mm long, 1.5* as long as hind tibia. Antennae brown. Femora wider than female femora. Marginal vein tan, stigma dark brown. Antennae flagellum about the same length as gaster, funicle length greater than width, anellus about 1/3 of pedicel length. Stigma 1.6 as long as width, stigmal vein short and 0.18* as long as stigma. Other morphology is similar to female. Diagnosis The key from China distinguished the females of Megastigmus daduheensis sp. n. by their body length less than 5 mm, ovipositor exceeding body length, stigma neck less than 0.5 times the width of the stigma and scultellum dark yellow. (Fig. 1A, G, E). Megastigmus daduheensis sp. n. is similar to Megastigmus duclouxianae Xu & He, 1995 (Fig. 2A, B) and Megastigmus sabinae Xu & He, 1989 (Fig. 3A,B), but can be distinguished from the latter two species by: 1) stigma light brown, 2.2* as long as width, stigma neck short and 0.11x as long as stigma; 2) ovipositor 4.5x as long as metatibia; and 3) mesosctum black anteriorly to brownish-yellow posteriorly. Figure 2. EES] Megastigmus duclouxianae, female; A Body, lateral view; B Head and mesosoma, dorsal view. 8 Chen X et al A B Figure 3. EU Megastigmus sabinae, female; A Body, lateral view; B Head and mesosoma, dorsal view. Etymology The new species is named after its type locality, Daduhe, Sichuan Province. Biology The eggs are laid in the cones of Cupressus chengiana S.Y. Hu, feeding on the nuts and the adults fly out of the cones from October to November every year. Identification keys Key to species of Megastigmus from China (females) 1 Body length greater than 5 mm M. sinensis Sheng, 1989 — Body length less than 5 mm 2 2 Ovipositor equals or exceeds body length 3 — Ovipositor shorter than body length 8 3 ae neck more than 0.5 times the width of the iM. aculeatus Swedetus. 1978 — Stigma neck less than 0.5 times the width of the stigma 4 4 Marginal vein shorter than postmarginal vein length 5 10 11 12 13 14 15 A new species of Megastigmus (Hymenoptera, Megastigmidae) from China Marginal vein equals or exceeds postmarginal vein length Scultellum dark brown Scultellum dark yellow Width of head about 1.17 times the length in the ventral view Head about twice as wide as long in the ventral view Pronotum with two yellow spots Pronotum without spots Mid-lobe of mesoscutum with short longitudinal ridge Mid-lobe of mesoscutum with transverse costulae Ovipositor longer than gaster length Ovipositor equal to or shorter than gaster length Scultellum black Scultellum yellow or brown Pedicel of antennae longer than F1 length Pedicel of antennae equal to or shorter than F1 length Marginal vein equal to postmarginal vein length Marginal vein shorter than postmarginal vein length Stigma neck length equal to the width of the stigma Stigma neck length shorter than the width of the stigma Body orange yellow Body yellowish-brown or light yellow Head with dark spots M. duclouxianae Xu & He, 1995 M. daduheensis sp. n. M. carinus Xu & He, 1995 M. likiangensis Roques & Sun, 1995 M. sp. (Host: Tsuga forresttii) M. ezomatsuanus Hussey & Kamijo, 1958 9 M. cellus Xu & He, 1995 10 11 13 M. lasiocarpae Crosby, 1913 12 M. sp. (Host: Sabina recurva var. COxil) M. pictus Foerster, 1841 M. pseuclotsugaphilus Xu & He, 1995 14 15 16 M. sabinae Xu & He, 1989 10 Chen X et al — Head without dark spots M. formosana Roques & Pan, 2005 Transscutal articulation of scutellum with irregular 16 ieulatedeulpture M. pseuclomali Xu & He, 1995 : Transscutal articulation of scutelluma with striped or 17 smooth sculpture 17 F7 more than twice as long as wide M. pingii Roques & Sun, 1995 — F7 less than twice as long as wide 18 18 Pronotum 1.9 times longer than width M. rigidae Xu & He, 1998 — Pronotum 1.1-1.3 times longer than width M. cryptomenae Yano, 1918 Analysis Phylogenetic relationship Megastigmus is sometimes difficult to classify accurately because of its small size and similar morphological characteristics. Therefore, the phylogenetic relationships are important to resolve species boundaries. Here, the existing mitochondrial data (CO/ + Cytb) was used to construct a molecular phylogenetic tree of Megastigmus, so as to verify the taxonomic status of the new species described above (Fig. 4). In terms of phylogenetic relationships, MW. daduheensis sp. nov. and M. sabinae were sister species and then they constituted one clade with M. duclouxiana. This result is consistent with morphological identification. Genetic distance In recent years, genetic distance has been regarded as one of the main characteristics of Hymenoptera species classification (Takahashi et al. 2018, Kitnya et al. 2022). The criteria proposed by Hebert et al. (2003) showed that the intraspecific genetic distance of invertebrate was less than 0.02 and the interspecific genetic distance was more than 10 times the intraspecific genetic distance (0.2). Thus, MEGA 7.0 was used to calculate pairwise genetic distances (Table 2) and common base pairs between species for 28 species of the Megastigmus, based on CO/ and Cytb. The results showed that the interspecific genetic distance base on the CO/ and Cytb were 0.01 ~ 0.60 and 0.02 ~ 0.59, with an average of 0.163 and 0.132, respectively. The genetic distances (CO//Cytb) of M. daduheensis sp. nov. with M. duclouxiana and M. sabinae were 0.56/0.55 and 0.54/0.57 and the percentages of shared base pairs (CO/+Cytb) were 76.3% and 76.8%, respectively. Molecular evidence confirmed M. daduheensis sp. nov. was distantly related to M. duclouxiana and M. sabinae and it was a separate species. A new species of Megastigmus (Hymenoptera, Megastigmidae) from China Figure 4. EES] Phylogenetic trees of Megastigmus inferred by Maximum Likelihood (ML) methods, based on mitochondrial data (CO/ + Cytb). Table 2. 27 57 69 43 22 31 68 40 92 41 100 65 66 82 100 85 89 68 98 71 67 100 99 59 M. dorsalis borriesi synophri stigmatizans zebrinus amicorum atlanticus bipunctatus cryptomeriae rosae thyoides spermotrophus hoffmeyeri M. pictus M. rafni M. tsugae M. atedius M. lasiocarpae M. milleri M. pinus M. pinsapinis M. suspectus M. schimitscheki M. duclouxiana M. sabinae SSSSRSESEEESEE 11 M. daduheensis sp. nov M. aculeatus M. strobilobius Torymus geranii Pairwise genetic distances of CO/ and Cytb amongst 28 species of Megastigmus. (COlil Cytb) 1 2 3 0.09/0.11 0.56/0.55 0.60/0.17 0.56/0.11 0.57/0.05 0.55/0.05 0.56/0.10 0.56/0.11 0.56/0.10 0.58/0.12 0.55/0.11 0.59/0.11 0.56/0.15 0.57/0.15 0.57/0.10 0.56/0.10 2 0.54/0.57 0.59/0.14 0.54/0.09 0.56/0.10 0.53/0.09 0.54/0.08 0.55/0.07 0.55/0.07 0.57/0.14 0.54/0.10 0.58/0.09 0.54/0.12 0.55/0.13 0.54/0.09 0.54/0.08 0.12/0.56 0.10/0.57 0.10/0.55 0.10/0.54 0.11/0.56 0.10/0.56 0.11/0.55 0.09/0.56 0.10/0.55 0.22/0.57 0.12/0.58 0.10/0.57 0.10/0.56 0.09/0.56 0.12/0.17 0.13/0.17 0.14/0.16 0.13/0.14 0.12/0.15 0.11/0.14 0.11/0.14 0.11/0.17 0.25/0.17 0.11/0.17 0.12/0.13 0.11/0.16 0.11/0.15 0.10/0.10 0.10/0.09 0.10/0.09 0.11/0.09 0.09/0.07 0.11/0.13 0.07/0.08 0.21/0.09 0.11/0.15 0.10/0.17 0.09/0.09 0.09/0.08 0.06/0.04 0.12/0.10 0.11/0.10 0.11/0.09 0.10/0.12 0.10/0.10 0.23/0.10 0.12/0.14 0.13/0.17 0.11/0.11 0.12/0.09 0.12/0.08 0.12/0.10 0.11/0.08 0.10/0.12 0.10/0.10 0.22/0.09 0.12/0.14 0.13/0.16 0.12/0.10 0.10/0.09 0.10/0.06 0.08/0.04 0.06/0.04 0.10/0.13 0.10/0.12 0.06/0.06 0.07/0.07 0.23/0.07 0.25/0.08 0.12/0.15 0.14/0.14 0.10/0.15 0.12/0.14 0.07/0.07 0.06/0.07 0.07/0.05 0.07/0.05 10 0.08/0.12 0.06/0.05 0.22/0.06 0.12/0.14 0.10/0.15 0.06/0.05 0.02/0.04 12 Chen X et al 18 0.57/0.11 0.55/0.09 0.10/0.56 0.12/0.17 0.09/0.09 0.11/0.11 0.12/0.10 0.07/0.07 0.07/0.07 0.06/0.05 19 0.57/0.10 0.55/0.09 0.11/0.56 0.12/0.16 0.09/0.09 0.12/0.10 0.12/0.09 0.09/0.06 0.08/0.07 0.06/0.05 20 0.56/0.10 0.55/0.08 0.10/0.55 0.10/0.14 0.09/0.08 0.10/0.08 0.12/0.08 0.08/0.05 0.07/0.05 0.07/0.04 21 0.56/0.12 0.54/0.11 0.09/0.56 0.10/0.17 0.09/0.11 0.10/0.10 0.09/0.10 0.09/0.10 0.10/0.08 0.08/0.09 22 0.57/0.10 0.54/0.09 0.09/0.55 0.12/0.13 0.08/0.10 0.11/0.09 0.11/0.08 0.07/0.06 0.09/0.06 0.07/0.05 23 0.58/0.07 0.56/0.11 0.12/0.56 0.14/0.20 0.11/0.11 0.07/0.06 0.06/0.06 0.13/0.09 0.12/0.11 0.10/0.09 24 0.55/0.10 0.53/0.07 0.08/0.55 0.11/0.14 0.09/0.08 0.10/0.08 0.10/0.07 0.07/0.04 0.07/0.03 0.06/0.03 25 0.56/0.13 0.54/0.12 0.10/0.57 0.11/0.17 0.12/0.13 0.11/0.11 0.13/0.12 0.09/0.11 0.10/0.11 0.09/0.11 26 0.56/0.12 0.54/0.10 0.10/0.55 0.10/0.16 0.09/0.11 0.12/0.11 0.13/0.09 0.06/0.06 0.07/0.07 0.07/0.05 27 0.56/0.09 0.54/0.07 0.09/0.56 0.10/0.14 0.09/0.09 0.11/0.09 0.11/0.08 0.05/0.03 0.07/0.05 0.06/0.04 28 0.56/0.11 0.54/0.08 0.09/0.56 0.11/0.15 0.07/0.08 0.09/0.09 0.09/0.08 0.07/0.05 0.07/0.06 0.05/0.04 (COW Cyt) 11 12 13 14 15 16 17 18 19 20 12 0.10/0.13 13 0.25/0.13 0.21/0.06 14 0.11/0.15 0.10/0.16 0.24/0.15 15 0.10/0.15 0.09/0.15 0.21/0.18 0.09/0.13 16 0.10/0.13 0.03/0.04 0.22/0.07 0.12/0.15 0.090.15 17 0.08/0.12 0.05/0.06 0.22/0.07 0.11/0.15 0.10/0.15 0.06/0.07 18 0.10/0.13 0.03/0.04 0.22/0.07 0.12/0.15 0.09/0.14 0.01/0.02 0.06/0.07 19 0.11/0.13 0.06/0.07 0.24/0.09 0.11/0.15 0.10/0.17 0.07/0.08 0.05/0.06 0.07/0.07 20 0.09/0.12 0.05/0.06 0.23/0.07 0.1/0.15 0.11/0.16 0.06/0.07 0.06/0.05 0.06/0.08 0.07/0.06 21 0.08/0.14 0.07/0.11 0.23/0.12 0.09/0.15 0.09/0.17 0.09/0.10 0.08/0.09 0.09/0.10 0.09/0.10 0.08/0.10 22 0.09/0.11 0.06/0.08 0.22/0.09 0.11/0.10 0.09/0.12 0.07/0.08 0.07/0.07 0.07/0.07 0.07/0.07 0.07/0.06 23 0.11/0.13 0.11/0.10 0.24/0.11 0.13/0.16 0.12/0.18 0.12/0.11 0.10/0.11 0.13/0.11 0.12/0.10 0.12/0.10 24 0.09/0.11 0.05/0.05 0.23/0.05 0.12/0.14 0.10/0.14 0.06/0.06 0.05/0.04 0.06/0.06 0.08/0.05 0.05/0.03 25 0.09/0.13 0.09/0.12 0.23/0.11 0.13/0.14 0.10/0.14 0.09/0.12 0.09/0.11 0.09/0.12 0.10/0.13 0.09/0.11 26 0.10/0.15 0.05/0.07 0.23/0.08 0.10/0.17 0.09/0.18 0.06/0.08 0.06/0.07 0.06/0.07 0.07/0.07 0.06/0.06 27 0.09/0.13 0.04/0.06 0.23/0.06 0.11/0.15 0.09/0.16 0.05/0.07 0.05/0.05 0.06/0.07 0.06/0.05 0.06/0.05 28 0.09/0.12 0.04/0.06 0.23/0.07 0.09/0.15 0.10/0.15 0.06/0.07 0.04/0.05 0.06/0.07 0.06/0.05 0.06/0.04 (CON Cytb) 21 22 23 24 25 26 27 22 0.07/0.09 23 0.10/0.11 0.11/0.10 24 0.07/0.08 0.07/0.05 0.11/0.09 25 0.09/0.11 0.10/0.10 0.12/0.13 0.10/0.10 26 0.08/0.11 0.07/0.08 0.12/0.12 0.06/0.05 0.08/0.12 27 0.08/0.10 0.07/0.07 0.11/0.09 0.06/0.03 0.08/0.11 0.04/0.06 28 0.06/0.09 0.05/0.06 0.10/0.10 0.06/0.03 0.08/0.10 0.06/0.05 0.05/0.05 Note: CO/ fragment before backslash (/) and Cytb fragment after backslash. 1 M. Sabinae; 2 M. duclouxiana; 3 M. daduheensis sp. nov.; 4 M. dorsalis; 5 M. aculeatus; 6 M. amicorum; 7 M. bipunctatus; 8 M. pinus; 9 M. spermotrophus; 10 M. tsugae; 11 M. zebrinu; A new species of Megastigmus (Hymenoptera, Megastigmidae) from China 13 12 M. schimitscheki, 13 M. strobilobius; 14 M. synophn; 15 M. stigmatizans; 16 M. pinsapinis; 17 M. atedius; 18 M. suspectus; 19 M. rafni; 20 M. pictus; 21 M. cryptomeriae; 22 M. thyoides; 23 M. atlanticus; 24 M. hoffmeyerl, 25 M. rosae; 26 M. lasiocarpae; 27 M. milleri; 28 M. borriesi. Discussion The identification of species based on morphological differences is essential to insect taxonomy. Nevertheless, the traditional classification method has drawbacks when it comes to tiny and similar-looking species, while molecular data can make up for the shortcomings of this method. Currently, many studies have stated explicitly the appropriateness of mtDNA in resolving the relationships amongst subspecies or closely- related species (Sperling and Hickey 1995, Davison et al. 2001, Wahlberg et al. 2003). For instance, Megastigmus spermotrophus nigrodorsatus has been considered until now as a subspecies of MV. spermotrophus, but molecular results (mean distances of 3.3% in Cytb and 0.3% in 28S) suggested that the species actually could be a distinct species rather than a subspecies of M. spermotrophus (Auger-Rozenberg et al. 2010). Similarly, two specimens from Greece and Kenya were identified as Megastigmus pistaciae, but Roques et al. (2017) study showed that they diverged by 4.5% in the CO/ data and the two are probably sibling species. Therefore, the combination of the two methods can make insect identification more accurate, which is of great significance for improving the efficiency and accuracy of species identification. In this paper, morphological characters and molecular data (CO/ and Cytb) were used to Classify parasitic wasps from Cupressus chengiana S.Y. Hu. Both methods confirm that M. daduheensis sp. nov. is a separate species, which is clearly different from other species in the genus and has a certain degree of diversification. Although M. daduheensis sp. n. was similar to M. sobinae and M. duclouxiana in morphology, it can be distinguished from other species, based on characters on the mesoscutum, stigma and ovipositor. MW. daduheensis sp. nov. and M. sabinae were sister species, based on the phylogenetic relationship and genetic distance and, together, they constituted one clade with M. duclouxiana. This result is consistent with previous studies and supports Megastigmus on Cupressaceae plants formed a distinct clade (Auger-Rozenberg et al. 2010). Roques et al. (2017) has calculated intra-group and inter-group distances of Megastigmus, based on the CO/ dataset and the lower values were observed within groups rather than between groups, with values ranging from 6% to 7.9%. However, in our study, the average pairwise genetic distance (CO//Cytb: 0.16/0.13) of Megastigmus species was higher and the genetic distance of VW. daduheensis sp. nov. supported it as a separate species. This study not only increased the species diversity of Wegastigmus, but also provided the genetic information of M. daduheensis sp. nov. and enriched the genetic database. However, our study only obtained CO/ and Cytb genes of this genus, which contained incomplete genetic information. Therefore, it can be combined with other mitochondrial or nuclear genes for a comprehensive analysis and verification in the future. 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