Biodiversity Data Journal 11: e99980 CO) doi: 10.3897/BDJ.11.e99980 open access Taxonomy & Inventories Note on the genus Serendib Deeleman-Reinhold, 2001, with the description of a new species (Araneae, Corinnidae, Castianeirinae) Lu Zhang?, Feng Zhang* + The Key Laboratory of Zoological Systematics and Application, Institute of Life Science and Green Development, College of Life Sciences, Hebei University, Baoding, China Corresponding author: Feng Zhang (dudu06042001@163.com) Academic editor: Jan Bosselaers Received: 06 Jan 2023 | Accepted: 01 Feb 2023 | Published: 15 Feb 2023 Citation: Zhang L, Zhang F (2023) Note on the genus Serendib Deeleman-Reinhold, 2001, with the description of a new Species (Araneae, Corinnidae, Castianeirinae). Biodiversity Data Journal 11: e99980. https://doi.org/10.3897/BDJ.11.e99980 ZooBank: urn:lsid:zoobank.org:pub:93991A8D-3D2E-444D-AFD4-495CA0787948 Abstract Background Prior to this study, the genus Serendib Deeleman-Reinhold, 2001 has been discovered in Indonesia, Thailand and Laos and comprises three species: S. muadai Jager, Nophaseud & Praxaysombath, 2012, S. suthepica Deeleman-Reinhold, 2001 and S. volans Deeleman- Reinhold, 2001. New information The genus Serendib Deeleman-Reinhold, 2001 is reviewed. A new species, S. hispida sp. n. (male and female), representing the first record of genus Serendib from Malaysia, is described. Descriptions and illustrations of the females of S. volans (Malaysia) and S. suthepica (China) are also provided. The latter represents the first record of the genus in China. © Zhang L, Zhang F. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 2 Zhang L, Zhang F Keywords Dionycha, morphology, spiders, taxonomy Introduction Deeleman-Reinhold (2001) erected the genus Serendib, based on the type species Serendib volans Deeleman-Reinhold, 2001 and placed it in Castianeirinae, Corinnidae. Meanwhile, another species S. suthepica Deeleman-Reinhold, 2001 was described somatically for both sexes, but without detailed descriptions of genital characteristics (Deeleman-Reinhold 2001). Subsequently, the third species, S. muadai Jager, Nophaseud & Praxaysombath, 2012 was described and illustrated (Jager et al. 2012). While examining the corinnids specimens collected from the Oriental Region during the past decade, we found one species that is consistent with the generic characteristics of Serendib, namely S. hispida sp. n., representing the first record of Serendib from Malaysia. The species, Serendib volans and S. suthepica are redescribed, with the latter recorded from China for the first time, representing the northernmost record for Serendib. Descriptions and illustrations are provided for the females of two known species and both sexes of one new species. Materials and methods All measurements are given in millimetres (mm). Leg measurements are shown as total length (femur, patella, tibia, metatarsus, tarsus). Epigynes were removed and cleared in a pancreatin solution (Alvarez-Padilla and Hormiga 2007). All specimens are preserved in 95% alcohol and were examined, illustrated and measured with a Leica M205A stereomicroscope. Somatic photographs were captured using a Leica M205A stereomicroscope, equipped with a DFC550 CCD camera and morphology photographs were taken using an Olympus BX51 microscope equipped with a Kuy Nice CCD with a Canon 60 mm micro-lens and were imported into Helicon Focus 7 for stacking. Drawings were used with Inkscape version 1.0.2.0. Final figures were retouched with Adobe Photoshop CC © 2022. The distribution map was made using ArcGIS Desktop version 10.6. The specimens used in this study are deposited in the Museum of Hebei University, Baoding, China (MHBU). The abbreviations used in the text are as follows: Eyes: ALE = anterior lateral eye; AME anterior median eye; MOA = median ocular area; PLE = posterior lateral eye; PME posterior median eye; RTA = retrolateral tibial apophysis. Note on the genus Serendib Deeleman-Reinhold, 2001, with the description ... 3 Taxon treatments Serendib Deeleman-Reinhold, 2001 Type species Serendib volans Deeleman-Reinhold, 2001 Description Small spiders, 4-6 mm in length. Carapace wedge-shaped or elongated, smooth or with plumose hairs; several long setae on clypeus and eye region; broadest of carapace at coxae Il-lll (Fig. 7a); anterior eye row slightly recurved in dorsal view and posterior eye row very wide and strongly recurved (Fig. 1a); fovea short, longitudinal, indistinct. Chelicerae same colour as carapace, both promargin and retromargin with two teeth. Sternum about as broad as long, slightly narrowed posteriorly. Leg formula 4123; femora I-Il with one row of long bristles ventrally (usually four or eight), femora III-IV with one or two long bristles ventrally (Fig. 3). Abdomen round or oval in females, elongate oval in males; with short or long grooved collar (Fig. 1b—d); anterior with one or two pairs of strongly erected spines (Fig. 2); dorsal scutum strongly sclerotized (Fig. 7a). Figure 1. Characteristics of Serendib species. Scale bars: 0.2 mm. a: Serendib hispida sp. n.: male ocular area, dorsal view (left) and frontal view (right); EES] b: Same, female collar; EES] c: Serendib suthepica Deeleman-Reinhold, 2001: female collar; ERY d: Serendib volans Deeleman-Reinhold, 2001: female collar. EE Zhang L, Zhang F Figure 2. Erect spines on anterior abdomen of Serendib species, females. Scale bars: 1 mm. a: Serendib hispida sp. n., dorsal view; ERS b: same, lateral view; EE] c: Serendib volans Deeleman-Reinhold, 2001, dorsal view; EE d: same, lateral view. EE Figure 3. Femora of Serendib species (retrolateral view), females. Scale bars: 0.2 mm. a: Serendib hispida sp. n.; ERS] b: Serendib suthepica Deeleman-Reinhold, 2001; ETS] c: Serendib volans Deeleman-Reinhold, 2001. ERS Note on the genus Serendib Deeleman-Reinhold, 2001, with the description ... {| / c : — d — Figure 4. EG Serendib volans Deeleman-Reinhold, 2001: a female habitus, dorsal view; b same, ventral view; c epigyne, ventral view; d vulva, dorsal view. Abbreviations: CO—copulatory opening; CD—copulatory duct; FD—fertilization duct; S—spermatheca. Scale bars: 1 mm (a—b); 0.1 mm (c-—d). oy ° tr Figure 5. EES] Living habitus of Serendib suthepica Deeleman-Reinhold, 2001: a—b female, incomplete egg capsule and eggs (black arrows) (photographs by Kun Yu). Zhang L, Zhang F Figure 6. El Serendib suthepica Deeleman-Reinhold, 2001: a female habitus, dorsal view; b same, ventral view; c epigyne, ventral view; d vulva, dorsal view. Abbreviations: CO—copulatory opening; CD—copulatory duct; FD—fertilization duct; S—spermatheca. Scale bars: 1 mm (a—b); 0.2 mm (c—d). \ s c Figure 7. EES Serendib hispida sp. n.: a male habitus, dorsal view; b same, ventral view; c female habitus, dorsal view; d same, ventral view. Scale bars: 1 mm (a—d). Palpal tibia longer than wide, with retrolateral hump and small RTA. Subtegulum visible in retrolateral view. Sperm duct coiled and formed loops, anterior with extra transverse Note on the genus Serendib Deeleman-Reinhold, 2001, with the description ... 7 U-shaped loop in S. hispida sp. n. and S. suthepica. Embolus with distinctly narrowed tip (Fig. 8a—d and fig. 517 in Deeleman-Reinhold (2001)). Figure 8. EESI Serendib hispida sp. n.: a male left palp, prolateral view; b same, ventral view; c same, retrolateral view; d enlarged emblous, ventro-retrolateral view; e epigyne, ventral view; f vulva, dorsal view. Abbreviations: E—embolus; T—tegulum; ST—subtegulum; SD—sperm duct; RTA —retrolateral tibial apophysis; CO—copulatory opening; CD—copulatory duct; FD— fertilization duct; S—spermatheca. Scale bars: 0.1 mm (a-f). Epigyne simple and strongly sclerotized. Copulatory openings posteriorly situated, with simple sclerotized margins. Copulatory ducts gradually extended into anterior spermathecae. Posterior spermathecae separated or contiguous (Fig. 4c—d; Fig. 6c—d; Fig. 8e-f). Diagnosis All species of Serendib resemble those of Sphecotypus O. Pickard-Cambridge, 1895 and Aetius O. Pickard-Cambridge, 1897 in ant-mimicking, small body size and having wide, strongly recurved posterior eye row (Fig. 1a), but can be distinguished by the following characters: 1) abdomen without transverse constriction (Fig. 4a—b; Fig. G6a—b; Fig. 7a, Cc); 2) anterior abdomen with one or two pairs of strongly erected spines (Fig. 2 ); 3) femora I-Il with one row of long bristles ventrally (usually four or eight), femora Ill- IV with one or two long bristles ventrally (Fig. 3); 4) copulatory duct long, curved or spiral (Fig. 4d; Fig. 6d; Fig. 8f). Distribution China, Indonesia, Laos, Malaysia, Thailand (Fig. 9). 8 Zhang L, Zhang F ut kor a ) 0 255510 1,020 1,530 2040 , a ees Kilometers Figure 9. EES Distribution map of the genus Serendaib. Serendib volans Deeleman-Reinhold, 2001 Material a. scientificName: Serendib volans Deeleman-Reinhold, 2001; country: Malaysia; stateProvince: Negeri Sabah; locality: Forest Girl Camp; year: 2017; month: 5; day: 2; individualCount: 1; sex: female; lifeStage: adult; recordedBy: Chi Ji; institutionID: the Museum of Hebei University; institutionCode: MBHU; occurrence!D: 97B26255-0FE4-505D-8BE4-85D25C01 7548 Description Female (Fig. 4a—b). Total length 5.32; carapace 2.07 long, 1.44 wide; abdomen 3.25 long, 1.76 wide. Eye sizes and interdistances: AME 0.13, ALE 0.10, PME 0.11, PLE 0.09; AME-AME 0.22, AME-ALE 0.14, ALE-ALE 0.47, PME-PME 0.29, PME—PLE 0.21, PLE-PLE 0.65, ALE—-PLE 0.18. MOA 0.22 long, anterior width 0.31, posterior width 0.37. Clypeal height 0.13. Labium 0.12 long, 0.31 wide. Sternum 0.93 long, 0.81 wide. Measurements of legs: | 5.18 (1.46, 0.46, 1.22, 1.11, 0.93), Il 5.01 (1.44, 0.47, 1.17, 1.05, 0.88), Ill 4.84 (1.43, 0.45, 1.12, 1.11, 0.73), IV 6.32 (1.83, 0.49, 1.53, 1.55, 0.92). Carapace brown, with smooth surface (Fig. 4a). Legs slender, brown; femora I-II black, with two white and longitudinal stripes (Fig. 4a—b); femora I-IIl with long bristles Note on the genus Serendib Deeleman-Reinhold, 2001, with the description ... FS) ventrally (Fig. 3c). Abdomen dark brown and round, with a pair of sigilla; with long and transverse groove collar (Fig. 4a; Fig. 1d); anteriorly with one pair of strongly erected spines (Fig. 2b, d). Dorsal scutum large, round, nearly covered abdomen, with a pair of white stripes (Fig. 4a). Epigyne as in Fig. 4c—d. Copulatory openings distinct, with arc-shaped sclerotized margins. Copulatory ducts basal wide, gradually narrowed and extended into anterior spermathecae. Diagnosis The female of S. volans resembles that of S. muadai in having similar colouration and habitus, but can be distinguished by the following characteristics: 1) abdomen with a pair of sigilla (vs. absent in S. muadai) (cf. Fig. 4a with fig. 55 in Jager et al. (2012)); 2) posterior spermathecae oval, distinctly separated (vs. posterior soermathecae round, closed to each other in S. muadai) (cf. Fig. 4d with fig. 45 in Jager et al. (2012)). Distribution Thailand, Malaysia (Borneo) (Fig. 9). Serendib suthepica Deeleman-Reinhold, 2001 Materials a. scientificName: Serendib suthepica Deeleman-Reinhold, 2001; country: China; stateProvince: Yunnan Province; county: Jinghong; locality: Wild Elephant Valley; verbatimElevation: 800m; verbatimLatitude: 22°10'24.12"N; verbatimLongitude: 100°51'33.75"E; year: 2021; month: 8; day: 4; individualCount: 3; sex: female; lifeStage: adult; recordedBy: Kun Yu; institution!|D: the Museum of Hebei University; institutionCode: MHBU; occurrence!D: A4BFC04E-8B8F-5140-962E-E66E4586DDC2 b. scientificName: Serendib suthepica Deeleman-Reinhold, 2001; country: China; stateProvince: Yunnan Province; county: Jinghong; locality: Wild Elephant Valley; verbatimElevation: 814m; verbatimLatitude: 22°10'25.05"N; verbatimLongitude: 100°51'19.07"E; year: 2022; month: 6; day: 5; individualCount: 1; sex: female; lifeStage: adult; recordedBy: Lu Zhang; institution!D: the Museum of Hebei University; institutionCode: MHBU; occurrence!D: 5395DD71-5DB3-51C6-ACEA-D2193C1D1651 Description Female (Fig. 5; Fig. 6a—b). Total length 5.43; carapace 2.78 long, 1.52 wide; abdomen 2.65 long, 1.82 wide. Eye sizes and interdistances: AME 0.10, ALE 0.09, PME 0.09, PLE 0.08; AME—AME 0.30, AME-ALE 0.15, ALE—-ALE 0.60, PME-—PME 0.42, PME— PLE 0.37, PLE-PLE 1.04, ALE-PLE 0.38. MOA 0.26 long, anterior width 0.42, posterior width 0.51. Clypeal height 0.23. Labium 0.17 long, 0.33 wide. Sternum 1.10 long, 0.86 wide. Measurements of legs: | 4.97 (1.51, 0.47, 1.16, 1.04, 0.79), Il 4.82 (1.51, 0.42, 1.20, 1.02, 0.67), III 3.78 (1.50, 0.45, 1.19, 1.05, 0.59), IV 6.82 (2.20, 0.57, 1.70, 1.52, 0.83). 10 Zhang L, Zhang F Carapace black, elongated, covered plumose hair (Fig. 6a). Legs slender, with spines; coxae Il-lIll white, others black; Legs I-Il brown, femora black, distally with white stripes; Legs III-IV black, femur III with one white stripe distally, femur IV with one thin yellowish stripe distally (Fig. Ga—b); femora I-Il with five bristles ventrally, femur III with two, femur IV with one (Fig. 3b). Abdomen black, oval, covered golden hairs; with transverse strips anteriorly and medially; with short and grooved collar (Fig. 6a; Fig. 1 b). Dorsal scutum large, anterior with two pairs of strong spines (Fig. 6a). Epigyne as in Fig. 6c—d. Copulatory openings distinct, with straight sclerotized margins. Copulatory ducts transverse, coiled, gradually extended into large, black anterior spermathecae. Posterior spermathecae diverging, with ovate distal parts and connecting with fertilization ducts. Diagnosis See the diagnosis of S. hispida sp. n.. Distribution Thailand, Indonesia (Bali), China (Yunnan) (Fig. 9). Serendib muadai Jager, Nophaseud & Praxaysombath, 2012 Diagnosis and descriptions: Diagnosis, descriptions and illustration are provided by Jager et al. (2012). Distribution Laos (Fig. 9). Serendib hispida Zhang & Zhang, sp. n. ZooBank 7801B957-F982-4D6A-9874-88A34CA86AAC Materials Holotype: a. scientificName: Serendib hispida; country: Malaysia; county: Negeri Pahang; locality: Karak; verbatimElevation: 68m; verbatimLatitude: 3°25'53.88"N; verbatimLongitude: 102°3'40.74"E; year: 2015; month: 10; day: 27; individualCount: 1; sex: male; lifeStage: adult; recordedBy: Zhizhong Gao; institution|D: the Museum of Hebei University; institutionCode: MHBU; occurrence!D: DOO8FE69-6F 16-505D-B06B-614E3D6B23E8 Paratype: a. scientificName: Serendib hispida; country: Malaysia; county: Negeri Pahang; locality: Pahang; verbatimElevation: 68m; verbatimLatitude: 3°25'53.88"N; verbatimLongitude: 102°3'40.74"E; year: 2015; month: 10; day: 27; individualCount: 1; sex: female; lifeStage: Note on the genus Serendib Deeleman-Reinhold, 2001, with the description ... 11 adult; recordedBy: Zhizhong Gao; institution|D: the Museum of Hebei University; institutionCode: NHBU; occurrence!D: FAB32914-FE13-5027-8608-2F 3886291 DD7 Description Male (Holotype) (Fig. 7a—b). Total length 4.02; carapace 1.87 long, 1.29 wide; abdomen 2.15 long, 1.23 wide. Eye sizes and interdistances: AME 0.11, ALE 0.09, PME 0.10, PLE 0.08; AME-AME 0.19, AME-ALE 0.13, ALE-ALE 0.42, PME—-PME 0.25, PME-PLE 0.17, PLE—-PLE 0.56, ALE—PLE 0.20. MOA 0.18 long, anterior width 0.28, posterior width 0.35. Clypeal height 0.12. Labium 0.10 long, 0.27 wide. Sternum 0.86 long, 0.77 wide. Measurements of legs: | 4.68 (1.34, 0.47, 1.08, 0.96, 0.83), Il 4.61 (1.35, 0.34, 1.12, 0.99, 0.81), Ill 4.43 (1.29, 0.42, 1.03, 1.04, 0.65), IV 5.87 (1.65, 0.47, 1.36, 1.45, 0.94). Carapace brown, wedge-shaped, with smooth surface. Legs slender, orange (Fig. 7a— b); femur | with six bristles ventrally, femur II with five, femur III with two, femur IV with one (Fig. 3a). Abdomen dark brown, oval, with dorsal scutum; with short, V-shaped and grooved collar. Dorsal scutum large, oval; anterior with two pairs of strong spines and posterior with two rows of white, erected setae (Fig. 7a, c; Fig. 2a, c). Epigastric sclerite extending anteriorly and sclerotized. Ventral scutum rectangular and heavily sclerotized (Fig. 7b). Palp as in Fig. 8a—d. RTA subuliform, small. Sperm duct coiled and formed several loops; anterior with transverse U-shaped loop, middle double S-shaped, posterior with extra loop. Embolus straight spine-like process, with cataphracted stripes on surface. Female (Fig. 7c—d). Total length 5.32; carapace 2.07 long, 1.44 wide; abdomen 3.25 long, 1.76 wide. Eye sizes and interdistances: AME 0.13, ALE 0.10, PME 0.11, PLE 0.09; AME-AME 0.22, AME-ALE 0.14, ALE—ALE 0.47, PME-PME 0.29, PME—PLE 0.21, PLE-PLE 0.65, ALE-PLE 0.18. MOA 0.22 long, anterior width 0.31, posterior width 0.37. Clypeal height 0.13. Labium 0.12 long, 0.31 wide. Sternum 0.93 long, 0.81 wide. Measurements of legs: | 5.18 (1.46, 0.46, 1.22, 1.11, 0.93), Il 5.01 (1.44, 0.47, 1.17, 1.05, 0.88), Ill 4.84 (1.43, 0.45, 1.12, 1.11, 0.73), IV 6.32 (1.83, 0.49, 1.53, 1.55, 0.92). Other characteristics as in the holotype, except dorsal scutum extending about half the length of abdomen. Epigyne as in Fig. 8e-f. Copulatory openings posteriorly situated and separated. Copulatory ducts long, stair-stepping and extended into black anterior spermathecae. Posterior spermathecae slender, subcylindrical, separated. Fertilization ducts short, semi-circular, lying the posterior spermathecae. Diagnosis The new species resembles that of S. suthepica in anterior abdomen with two pairs of strong spines, but can be distinguished by the following characteristics: 1) the longer embolus (vs. short in S. suthepica) (cf. Fig. 8a—d with figs 517-519 in Deeleman- Reinhold (2001)); 2) carapace brown and smooth (vs. black, elongated, covered 12 Zhang L, Zhang F yellowish plumose in S. suthepica) (cf. Fig. 7a, c with Fig. 6a); 3) legs orange (vs. brown or black with white stripes distally in S. suthepica) (cf. Fig. 7 with Fig. Ga—b); 4) copulatory ducts long, transverse and stair-stepping (vs. transverse, coiled in S. suthepica) (cf. Fig. 8e—f with Fig. 6c—d). Etymology The specific name is an adjective and refers to the characters of the dorsal scutum with two rows of setae. Latin hispida = hispid. Distribution Malaysia (Pahang) (Fig. 9). Acknowledgements Thanks to Drs Chi jin, Drs Zhizhong Gao and Kun Yu for collecting valuable specimens. This work was supported by the National Natural Science Foundation of China (No. 32170468) and by the Science & Technology Fundamental Resources Investigation Program (No. 2022FY202100). References ° Alvarez-Padilla F, Hormiga G (2007) A protocol for digesting internal soft tissues and mounting spiders for scanning electron microscopy. Journal of Arachnology 35 (3): 538-542. https://doi.org/10.1636/Sh06-55.1 ° Deeleman-Reinhold CL (2001) Forest spiders of South East Asia: with a revision of thesac and ground spiders (Araneae: Clubionidae, Corinnidae, Liocranidae, Gnaphosidae, Prodidomidae and Trochanterriidae). Brill, Leiden 591 pp. https://doi.org/ 10.1163/9789004475588 ° Jager P, Nophaseud L, Praxaysombath B (2012) Spiders from Laos with description of a new species and new records (Arachnida: Araneae). Acta Arachnologica 61: 77-92. https://doi.org/10.2476/asjaa.61.77