Research Article Journal of Orthoptera Research 2018, 27(2): 163-171 Morphometric variation among males of Orphulella punctata (De Geer, 1773) (Acrididae: Gomphocerinae) from different biomes in Brazil ANA CATIA SANTOS DA SILVA!, LORENA ANDRADE Nunes?, WANESSA DE LIMA BatistA!, MARCOS GONCALVES LHANO! 1 Universidade Federal do Recéncavo da Bahia (UFRB), Centro de Ciéncias Agrarias, Ambientais e Biol6gicas (CCAAB), Programa de Pés-Graduacao em Ciéncias Agrarias. 44380-000. Cruz das Almas, BA, Brazil. 2 Universidade Estadual do Sudoeste da Bahia (UESB), Programa de P6s-Graduacao em Enfermagem e Satide. 45208-091. Jequié, BA, Brazil. Corresponding author: Marcos Goncalves Lhano (entomology@gmail.com) Academic editor: Maria-Marta Cigliano | Received 26 September 2017 | Accepted 1 October 2018 | Published 5 December 2018 http://zoobank.org/DFC1EBE8-EDC6-48A3-B20D-EE9DA5142F07 Citation: Silva ACS, Nunes LA, Batista WL, Lhano MG (2018) Morphometric variation among males of Orphulella punctata (De Geer, 1773) (Acrididae: Gomphocerinae) from different biomes in Brazil. Journal of Orthoptera Research 27(2): 163-171. https://doi.org/10.3897/jor.27.21203 Abstract The objective of the present study was to examine variation in the shape and size of pronotum, hind femur, and head in the males of Or- phulella punctata (De Geer, 1773) from three different Brazilian biomes: the Cerrado, the Atlantic Forest, and the Pantanal. A total of 150 specimens were analyzed from three populations. The results of MANOVA indicated significant differences (p<0.01) in the shape of the analyzed structures of O. punctata from the different biomes. The results of ANOVA demonstrated sig- nificant differences (p<0.05) in the size of all analyzed structures. Pearson's correlation analyses among the different structures and the environmen- tal variables revealed that the shape of pronotum, hind femur, and head (dorsal view), as well as the size of pronotum and head (dorsal and lateral views) varied with the geographic longitude, while the shape of hind femur and head (dorsal view) showed a significant negative correlation with size. Results indicated that the shape and size of the analyzed structures, in gen- eral, were influenced by the geographical variables. Key words geometric morphometrics, hind femur, Orthoptera, population analysis, pronotum, shape, size Introduction Grasshoppers are characterized by diverse forms and colors, and a range of ecological and economic importance (Sperber et al. 2012) particularly due to their potential for causing damage to agriculture, as certain grasshopper species are phytophagous with defoliating behaviors (Carrano-Moreira 2015). Among such spe- cies, Orphulella punctata (De Geer) shows a wide geographical dis- tribution ranging from Mexico to Argentina (Cigliano et al. 2018). This species is common in both native and anthropogenic areas and is considered a pest that causes minor damages to agricultural crops in Brazil (Guerra et al. 2012). Gomphocerine grasshoppers may be identified by characters of their external morphology (Otte 1979). However, in this subfamily as well as in other grasshopper groups, individuals of the same species occupying different geographical locations may exhibit morphological variation in several structures of their external morphology (Pocco et al. 2014). Geometric morphometrics is a technique that has been used widely in studies conducted on morphological variations at the intraspecific level throughout the geographical distribution range of species (Rattanawannee et al. 2012, Zelditch et al. 2012). Geometric morphometrics is a tool for detecting morphological variation among the organisms through the identification of landmarks and the subsequent evaluation of the relationships between these landmarks and certain other variables, such as environmental or geographical variables (Klingenberg 2013). This technique also allows the detection of significant differences in the shape and size of body structures with the objective of revealing intraspecific and interspecific variation that may be associated with evolutionary and ecological factors (Bower and Piller 2015). Therefore, this technique has become useful for the identification of variation among the populations of a given species (Oleksa and Tofilski 2015). Geometric morphometrics also enables the analysis of correlations between the form and size of structures and the patterns of distribution of individuals across various geographical areas as well as with the patterns of diversification of their life histories. As morphometric variations among insect populations are generally associated with differences in geographical and environmental variables, this methodology has proved to be very useful in this group of organisms (Nunes et al. 2012, Romero et al. 2014, Prado-Silva et al. 2016). Although geometric morphometrics has been demonstrated to be effective in analyzing morphological variation in insects, this methodology has been underused in studies of Orthoptera (Song and Wenzel 2008, Song 2009, Bidau et al. 2012, Cisneiros et al. 2012). In the present paper we analyze morphometric variation in males of O. punctata belonging to populations collected from three different biomes in Brazil. Material and methods Study area.—The specimens were collected from three Brazilian biomes: the Cerrado, the Pantanal, and the Atlantic Forest. Study JOURNAL OF ORTHOPTERA RESEARCH 2018, 27(2) 164 sites were located in: Serra da Bodoquena, Bonito, MS (21°15'56"S, 56°42'10"W); Estrada Porto Cercado, located between river Bento Gomes and the Pantanal Advanced Research Base (BAPP) of the Federal University of Mato Grosso, Poconé, MT (16°18'55.01'S, 56°32'33.64"W); and the Fazenda Baixa de Areia, Highway BA-026, Serra da Jiboia, Varzedo, BA (12°57'41.9"S, 39°26'54.9"W) (Fig. 1). According to the Brazilian Ministry of Environment (MMA), the Cerrado and the Atlantic Forest are considered biomes of great interest as they are rich in biodiversity and contain several en- demic species. The Pantanal is characterized by pronounced wet seasons and is the smallest biome in the country, occupying just 1.76% of the total land area (MMA 2017). Sampling.—In order to perform the morphometric analyses, a total of 150 O. punctata males (50 individuals from the Cerrado, 50 from the Atlantic Forest, and 50 from the Pantanal) were includ- ed in the study, except for the analysis of femur structure, where only 34 individuals from the Cerrado biome were included. All the specimens were deposited in the Laboratory of Ecology and Taxonomy of Insects (LETI), Biological Sciences Section, Center for Agricultural, Environmental and Biological Sciences (CCAAB), Universidade Federal do Recéncavo da Bahia (UFRB). Analyses.—The pronotum (left lateral view, Fig. 2A), hind femur (left lateral view, Fig. 2B), and head (left lateral and dorsal views, Fig. 2C, D) were photographed using Zeiss Discovery V20.0 stereo microscope with an attached camera. The TPS series of programs (Rohlf 2006) were used for the analysis of shape and size, and the images were converted in the TPSUtil program (Rohlf 2010). The landmarks and semi-landmarks (Fig. 2) were inserted using the TPSDig2 program (Rohlf 2006). The Cartesian coordinates were subjected to Procrustes superimposition, and Principal Compo- nent Analysis (PCA) and discriminant analysis were performed us- ing MorphoJ software (Klingenberg 2011). MANOVA was used for analyzing the differences in the shape of the structures and ANOVA was used for analyzing the differences in the size (centroid size) of the structures. Pearson’s correlations were performed in order to evaluate the relationships between the shape and size of the structures and the geographical variables (latitude, longitude, and altitude), using Past software v. 2.16 (Hammer et al. 2001). Addi- tionally, a cluster analysis was performed using the same program by applying the UPGMA method, and the cophenetic correlation coefficient was calculated. Results Analysis of pronotum shape in O. punctata.a—MANOVA results re- vealed significant differences (p<0.01) in the pronotum shape among the O. punctata males from the different biomes that were analyzed. The results of Hotelling’s test (T2) showed that the O. punctata males from the Atlantic Forest differed significantly in comparison to the males from the Pantanal and the Cerrado. The first four components explained 84% of the total variation in the pronotum shape. The first PCA explained 40%, the second one explained 24.8%, the third one 13.6%, and the fourth 5.6%. The greatest variation in the pronotum shape was observed in the dorsal and ventral posterior regions (Fig. 3A, B). The first PCA also revealed that the specimens from the Pantanal site differed in comparison to the populations from the other biomes (Fig. 3C). Specimens from the Pantanal site were located on the positive axis of PCA 2 and exhibited the greatest distortion in the posterior ventral region of pronotum which A.C.S. SILVA, L.A. NUNES, W.L. BATISTA AND M.G. LHANO -3000000 -2000000 Legend &\ Bonito-MS O Poconé-MT ( varzedo-BA Biomes 10000000 eg Caatinga (<3 Cerrado eo Pantanal LS Pampa Amazon Ge Atlantic Forest 9000000 3000000 7o00000 Fig. 1. Collection sites for Orphulella punctata (De Geer, 1773): Cerrado, Atlantic Forest and Pantanal. Fig. 2. Lateral view of Orphulella punctata (De Geer, 1773). A. Pro- notum: 10 anatomical points; B. Femur: 18 anatomical points; C. Lateral view of the head: 16 anatomical points; D. Dorsal view of the head: 18 anatomical points. Black circles represent the land- marks and white circles represent the semi-landmarks. expanded, as indicated by the Thin-Plate spline. On the other hand, this structure contracted in the individuals collected from the Cerrado and Atlantic Forest sites (Fig. 3C). Procrustes distance matrix showed a significant difference (p<0.01) in pronotum shape between the three biomes (Table 1). Table 1. Procrustes distance of Orphulella punctata pronotum shape among three Brazilian biomes. Values in lower half of dis- tance matrix and significance in top half of the distance matrix; 10,000 permutations. Cerrado Atlantic Forest Pantanal Cerrado 0 0.0004** 0.0001 ** Atlantic Forest 0.0270 0 0.0001 ** Pantanal 0.0622 0.0534 0 ** sionificant (p<0.01). JOURNAL OF ORTHOPTERA RESEARCH 2018, 27(2) A.C.S. SILVA, L.A. NUNES, W.L. BATISTA AND M.G. LHANO 165 B PC2 (-) + CERRADO C x ATLANTIC FOREST O PANTANAL gO TH 0.064 A Oo 14x o o.0 + o o SL AEl as a: oO OK oe O x ” OF g x are c 0.20 0.18 0.16 0.14 0.12 6.10 -0.08 0.06 DA 6 oa + 4 ae to.04* x 0.06 0.08 0.10 + +H nN x “a eit eae aa < Fy + x) Et x & oot + + -0.09+ + + + 0.12 -0.15- 0.184 PCA 1 Fig. 3. Scatter plot of the Principal Components Analysis (PCA) of Orphulella punctata (De Geer, 1773) pronotum shape in populations collected from the Cerrado, Atlantic Forest, and Pantanal. A. Thin-plate spline of the positive (+) and B. negative (-) axes of PCA 2; C. PCA plot. The UPGMA with 10,000 permutations and a cophenetic corre- lation coefficient value of 95% corroborated the above-mentioned results of PCA, demonstrating that the population from the Panta- nal was distantly related to the group that was constituted by the populations from the Atlantic Forest and the Cerrado (Fig. 4). Analysis of the shape of the hind femur.—MANOVA results demon- strated significant differences in the shape of hind femur among the populations from the different biomes (p<0.01). The first four PCAs accounted for 78.5% of the total variation in femur shape: the first PCA explained 54.8%, the second one explained 13.3%, the third one 6.1%, and the fourth 4.3%. It was possible to verify differences in the femoral structure among the O. punctata popula- tions from the analyzed biomes where specimens from the Cer- rado biome were separated from individuals from the other two biomes. On the positive axis of PCA 1, a contraction in the proximal region (points 2 and 17) of the femur in the individuals from the Pantanal and an expansion in the medial region (points 5, 6, 14, and 15) could be observed (Fig. 5A, C). The inverse of this oc- curred on the negative axis, i.e. an expansion in the proximal re- gion and a contraction in the medial region of the femur (Fig. 5B). The Procrustes distance matrix revealed significant differences in shape of the hind femur (p<0.01) among the studied popula- tions (Table 2). Similar to the PCA plot (Fig. 5C), UPGMA with 10,000 per- mutations and a cophenetic correlation coefficient value of 100% showed that the population from the Cerrado biome was distantly Atlantic Forest Cerrado Pantanal 0.008 0.016 0.024 0.032 Distan ce 0.040 0.048 0.056 100 Fig. 4. Similarity dendrogram for the pronotum in Orphulella puncta- ta populations from the Cerrado, Atlantic Forest, and Pantanal by the UPGMA method. The permutation test was carried out with 10,000 replicates and a cophenetic correlation coefficient of 97.1%. JOURNAL OF ORTHOPTERA RESEARCH 2018, 27(2) 166 A.C.S. SILVA, L.A. NUNES, W.L. BATISTA AND M.G. LHANO A B sf am na PC1 (+) PC1 ie (-) + CERRADO Cc x ATLANTIC FOREST + O PANTANAL 0.032 +e 0.024 +} a, ‘be x OP ee ake + at + 9.016 Ch cara = < + sh Hy By ne i is mH xX & -0.05-+ a oot 0.03 led rae aS 002 OX 03 [Jo.04 0.05 Oo “. x oR aa Se x (X Sele} FEI X x XD¢ x x 0.024 xX x 0.032 x Fig. 5. Scatter plot of the Principal Components Analysis (PCA) from Orphulella punctata (De Geer, 1773) femur shape in populations col- lected in the Cerrado, Atlantic Forest, and Pantanal. A. Thin-plate spline of the positive (+) and B. negative (-) axes of PCA 1; C. PCA plot. Table 2. Procrustes distance of Orphulella punctata femur shape among three Brazilian biomes. Values in lower half of distance matrix and significance in top half of the distance matrix; 10,000 permutations. Cerrado Atlantic Forest Pantanal Cerrado 0 0.0001 * * 0.0001 * * Atlantic Forest 0.0321 0 0.0073 ** Pantanal 0.0218 0.0113 0 ** significant (p<0.01). related to the group constituted by the populations from the At- lantic Forest and the Pantanal (Fig. 6). Analysis of the shape of the head in dorsal view.—MANOVA results revealed significant differences in the shape of head in dorsal view among the O. punctata populations from the different biomes (p<0.01). The PCA showed that the first four components explained 77.6% of the total variation: the first PCA explained 44.9%, the sec- ond one explained 13.9%, the third one 11.3%, and the fourth 7.5%. The major variations in the shape of head in dorsal view occurred in the distal region of the head (near the pronotum), in the medial portion, and in the fastigium. The greatest distortion occurred in the anterior and medial regions of the head, which expanded on the positive axis, as indicated in the deformation grids (Fig. 7A), and contracted on the negative axis (Fig. 7B). The second axis of the PCA revealed that the Cerrado popula- tion was slightly distinct from the populations of the other biomes (Fig. 7C). Atlantic Forest Pantanal Cerrado 0.000 0.003 0.006 0.009 100 0.012 0.015 Distan ce 0.018 0.021 0.024 0.027 100 Fig. 6. Similarity dendrogram for the femur from Orphulella punctata populations from the Cerrado, Atlantic Forest, and Pantanal by the UPGMA method. The permutation test was carried out with 10,000 replicates and a cophenetic correlation coefficient of 86.83%. JOURNAL OF ORTHOPTERA RESEARCH 2018, 27(2) A.C.S. SILVA, L.A. NUNES, W.L. BATISTA AND M.G. LHANO 167 A ~ B be | | [+ |e i a | IE , Fi : = el k cea dei ash ¥ ae jee ; i te 96 [TF ? Ez. PCc2 PC2 (+) (-) + CERRADO C x ATLANTIC FOREST PANTANAL + + 0.035 + 4 + 7 0.024 4 i‘ + ” x t as . +l v4 + aH x + x O Oo xt a + + Oo + + x at 01 + +h Xx x q x a x? & tpg X__ 4 + ox T T T sa al om =a + | T T -0.064 0.048 -0.032 -0.916F) a x o.biex 6032 0.048 < x xx x * Al % 5 - 3 04 Oo x Xk x Pm O = obh% mat x x Oo fo oO Ooo 0.03-+ Oo oO -0.045 D oO 0.05 o -0.06- PCA 1 Fig. 7. Scatter plot of the Principal Components Analysis (PCA) from Orphulella punctata (De Geer, 1773) dorsal head shape in populations collected in the Cerrado, Atlantic Forest, and Pantanal. A. Thin-plate spline of the positive (+) and B. negative (-) axes of PCA 2; C. PCA plot. Results from the Procrustes distance matrix demonstrated sig- nificant differences (p<0.01) in shape of the head in dorsal view among the populations (Table 3). The UPGMA with 10,000 permutations and a cophenetic cor- relation coefficient value of 97% corroborated the PCA results, showing that the population from the Cerrado biome was distant- ly related to the group constituted by the populations from the Atlantic Forest and the Pantanal (Fig. 8). Analysis of the shape of the head in lateral view.—MANOVA results indicated that the shape of the head in lateral view differed sig- nificantly among the O. punctata populations from the differ- ent biomes (p<0.01). The PCA demonstrated that the first four components explained 67.6% of the total variation in this trait. The first principal component explained 28.5%, the second one explained 20.6%, the third one 12.1%, and the fourth 6.4%. The greatest variation in the shape of the head in lateral view was Table 3. Procrustes distance of Orphulella punctata shape of the dorsal view of the head among three Brazilian biomes. Values in lower half of distance matrix and significance in top half of the distance matrix; 10,000 permutations. Cerrado Atlantic Forest Pantanal Cerrado 0 0.0001 * * 0.0001 * * Atlantic Forest 0.0187 0 0.0115* Pantanal 0.0262 0.0141 0 * significant (p<0.05), ** significant (p<0.01). 3 © ‘rl al ° Pa de a Bi a 4 a o ed a oO 0.0000 0.0025 0.0050 0.0075 0.0100 Es 0.0125 QO 0.0150 97 0.0175 0.0200 0.0225 = Fig. 8. Similarity dendrogram for the head in dorsal view for Or- phulella punctata populations from the Cerrado, Atlantic Forest, and Pantanal by the UPGMA method. The permutation test was carried out with 10,000 replicates and a cophenetic correlation co- efficient of 79.2%. JOURNAL OF ORTHOPTERA RESEARCH 2018, 27(2) 168 A.C.S. SILVA, L.A. NUNES, W.L. BATISTA AND M.G. LHANO A B (+) Pc2 + CERRADO GC x ATLANTIC FOREST O PANTANAL + oO | x 4 oO x x el te x oO - Oo Oo x Si T T T St T T T T T Fas -0.072 x cp-09° -0.948 To 036¢ o°: 0.048+ 0.060 0.072 0.084 ial os go x x x oo x e se x Oo oO O PCA 1 Fig. 9. Scatter plot of the Principal Components Analysis (PCA) from Orphulella punctata (De Geer, 1773) lateral head shape in populations collected in the Cerrado, Atlantic Forest, and Pantanal. A. Thin-plate spline of the positive (+) and B. negative (-) axes of PCA 2; C. PCA plot. observed near the occipital suture and in the eye. Although a uni- form distribution occurred, the major distortion was observed ® oO in the regions just mentioned, which contracted on the positive FE a 2 axis (Fig. 9A) and expanded on the negative axis (Fig. 9B). The [= 2 = second axis of the PCA did not represent sufficient differences =t o 5 to separate the populations from the different biomes (Fig. 9C). The Procrustes distance matrix revealed significant differences in the characteristics analyzed (p<0.01), indicating differences in oo the shape of head in lateral view among the populations (Table 4). The UPGMA with 10,000 permutations and a cophenetic cor- oe relation coefficient value of 92% showed that the population from the Cerrado biome formed an independent branch in the dendro- wee gram separated from the group constituted by the Pantanal and viele the Atlantic Forest populations (Fig. 10). 2 : # Analysis of the size of pronotum, hind femur, and head in Orphulella A ra punctata.—ANOVA analyses demonstrated significant differences nip in size among the populations from the different biomes (p<0.01) : #5 : 0.028 Table 4. Procrustes distance of Orphulella punctata shape of the lateral view of the head among three Brazilian biomes. Values in oon lower half of distance matrix and significance in top half of the : distance matrix; 10,000 permutations. 0.036 100 = ee Fig. 10. Similarity dendrogram for the lateral view of the head in Cerrado 0 0.0001 * * 0.0001 * * : : ph nce a avea Aeaaee 6 osoniee Orphulella punctata populations from the Cerrado, Atlantic Forest, and Pantanal by the UPGMA method. The permutation test was Fantanal’ OSG OST OT eaeried oueith 10,000 replicates and a cophenetic correlation co- ** significant (p<0.01). efficient of 98.8%. JOURNAL OF ORTHOPTERA RESEARCH 2018, 27(2) A.C.S. SILVA, L.A. NUNES, W.L. BATISTA AND M.G. LHANO for all the analyzed structures. Tukey's test confirmed that pro- notum and head in lateral view were larger in individuals from the Cerrado (Fig. 11A, D). The Atlantic Forest population had a larger hind femur in comparison to individuals from the other biomes (Fig. 11B), and the largest size of the head in dorsal view was found in the Pantanal (Fig. 11C). Correlation analyses between the shape and size of body structures and the geographical variables.—Pearson’s correlation analyses between the shape and size of the pronotum, hind femur, and head in O. punctata, and the geographical variables (latitude, longitude, and altitude) showed a significant negative correlation between shape x longitude, size x latitude, and size x longitude of the prono- tum. A negative correlation was observed between shape x lati- tude, shape x longitude, and shape ~x size of the hind femur. The head in dorsal view revealed a negative correlation between size x longitude and shape x size; while the head in lateral view showed a negative correlation between shape x longitude, size x latitude, and size x longitude (Table 5). These results indicated that the morphology of pronotum, hind femur, and head varied in rela- tion to the geographical variables. Centroid size oO Cerrado Pantanal Atlantic Forest Centroid size 4.0 Pantanal TA oO Say o aah — a = Centroid size 169 Discussion Among the evaluated structures in O. punctata, the hind fe- mur and the head in the population from the Cerrado biome dif- fered in comparison to the other populations from the remain- ing two biomes. The pronotum of the Pantanal population was significantly different from the other two populations. Nunes et al. (2007), while studying Melipona scutellaris Latreille (Hyme- noptera: Apidae), suggested that variation among geographically isolated populations might occur due to the existence of a barrier that prevents frequent migrations of individuals. For example, in bees, wing shape is influenced by geographical distance (Nunes et al. 2012, Lima et al. 2014, Prado-Silva et al. 2016). In a previous study, the relationship between shape and geographical distance was highlighted, suggesting that individuals distributed across dif- ferent latitudes, longitudes, and altitudes might present differenc- es that are associated with adaptations to local conditions (Berner et al. 2004). Similar patterns were observed in the present study, where the correlation analyses indicated that both the shape and the size of the morphological structures analyzed herein might be influenced by the geographical variables. o BE. = c D 7.6 b Atlantic Forest 7.2 6.8 6.4 6.0 a 5.6 Centroid size 5.2 4.8 4.4 4.0 Cerrado Pantanal Atlantic Forest Fig. 11. Analysis of the size of the A. pronotum, B. femur, C. head in dorsal view and D. head in lateral view. Similar letters indicate that these biomes are statistically equivalent in relation to the size of the pronotum, femur, dorsal, and lateral view of the head by Tukey’s test (p<0.05). JOURNAL OF ORTHOPTERA RESEARCH 2018, 27(2) 170 Table 5. Correlation between the shape and size of the pronotum, femur, and dorsal and lateral views of the head of Orphulella punc- tata with latitude, longitude, and altitude. R P Pronotum Shape x Latitude -0.0968 0.23837" Shape x Longitude -0.2295 0.0047 ** Shape x Altitude -0.1091 0.1837" Size x Latitude -0.3044 0.0001 ** Size x Longitude -0.2368 0.0035" Size x Altitude 0.2308 0.0044** Shape x Size -0.1516 0.0639"s Femur Shape x Latitude -0.6112 0.0000 ** Shape x Longitude -0.4583 0.0000 * Shape x Altitude 0.4414 0.0000 “* Size x Latitude 0.5311 0.0000 “* Size x Longitude 0.6829 0.0000 “* Size x Altitude -0.0160 0.8544"° Shape x Size -0.4916 0.0000 * Dorsal Head Shape x Latitude 0.0681 0.4075" Shape x Longitude -0.0316 0.7007" Shape x Altitude -0.1518 0.0639"° Size x Latitude -0.0934 W325 51" Size x Longitude -0.1876 0.0214* Size x Altitude -0.0651 0.4281" Shape x Size -0.2587 QO0013 4" Lateral Head Shape x Latitude -0.1412 0.0847"s Shape x Longitude -0.1590 0.0518 ° Shape x Altitude 0.0488 0.5526" Size x Latitude -0.3526 0.0000 * Size x Longitude -0.2239 0.0058" Size x Altitude 0.3272 0.0000°* Shape x Size 0.0080 0.9225" * significant (p<0.05), ** significant (p<0.01), "not significant. Intraspecific variation may complicate taxonomic studies, es- pecially when the variation occurs in the structures that have been used as taxonomic characters for identification, as is the case of the pronotum and head (fastigium) in O. punctata (Otte 1979). Intraspecific variation may also occur in the internal structures in grasshoppers, as demonstrated by Song and Wenzel (2008) in a study on the male genitalia morphology among three populations of Schistocerca lineata Scudder. Species that exhibit a wide geographical distribution tend to present both geographical variations and polymorphism, which may lead to incorrect identifications (Pocco et al. 2014). O. punc- tata is widely distributed from Mexico to Argentina, with polymor- phism as a common observation among its populations (Dixon 2005). Liebermann (1947) considered that O. punctata may be one of the acridid species with greatest variation in size and color. Rehn (1906) was the first to report the existence of variation in the structure and color among individuals belonging to different populations collected from diverse regions of Brazil and the US. Virgin Islands. In 1916, the same author (Rehn 1916) remarked on the existence of a number of variations among the populations of this plastic and widespread species collected from three different northern states of Brazil. Rehn (1918) then reported the existence of variation in the head and pronotum of the individuals belong- ing to two populations from Para, Brazil. Bruner (1913) also re- ported the existence of variation in size among the individuals of O. punctata collected from Peru. A.C.S. SILVA, L.A. NUNES, W.L. BATISTA AND M.G. LHANO Hebard (1923) described a remarkable variability of this species, especially in size, pronotal contour, and coloration, which has often led to misidentifications generating unusually complex synonymies (including the description of O. punctata populations as different species placed in different genera such as Oxycoryphus and Stenobo- thrus). Cigliano et al. (2018) reported 19 synonyms of O. punctata. In a taxonomic review of this group, Otte (1979) considered that O. punctata is easily misidentified with O. aculeata Rehn in central Mexico, and with O. losamatensis Caudell and O. concinnula (Walker) in Central and South America. The same author none- theless mentioned the importance of the hind femur and shape of the pronotum as the characteristics for distinguishing O. punctata from the remaining species of Orphulella. However, and according to the results obtained in the present study, the hind femur and pronotum also displayed variations among the populations from different biomes, and hence other structures and/or methods of identification are required for the purpose of a correct identifica- tion of this species. In addition to the structures suggested by Otte (1979) as di- agnostic characters for identification of O. punctata, the present study showed that the head also presented intraspecific variation, reinforcing the needs for other structures to be used in the identi- fication of this species. Intraspecific variations in the morphologi- cal characters, such as head, pronotum, femur, body, and wings, have also been reported in studies conducted on the populations of Chromacris speciosa (Thunberg) collected from two locations in Pernambuco, Brazil (Cisneiros et al. 2012). Also, analyses of the body size and the wings of Trilophidia annulata (Thunberg) (Orthoptera: Oedipodinae) demonstrated significant differences among the populations collected from different kinds of environ- ments in China (Bai et al. 2016), which corroborates the occur- rence of variations in individuals of the same species living in dif- ferent biomes. Whitman (2008) demonstrated that body size in Orthoptera varies both between and within species, mainly as a result of environmental factors. It is concluded that the shape and the size of the analyzed structures of O. punctata vary among biomes, indicating the possi- ble influence of environmental conditions on the variations in the morphology of this species. The geometric morphometrics analy- ses conducted in this study indicated that it is possible to separate the populations from different biomes by the shape and size of their various body parts. 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