Research Article Journal of Orthoptera Research 2024, 33(1): 87-93 Illustrated review of Mormon cricket Anabrus simplex (Tettigoniidae, Tettigoniinae) embryonic development RoserT B. SryGtey!, LAURA B. SENIOR! 1 Pest Management Research Unit, Northern Plains Agricultural Research Laboratory, USDA-Agricultural Research Service, 1500 N. Central Ave., Sidney MT 59270, USA. Corresponding author: Robert B. Srygley (robert.srygley @ars.usda.gov) Academic editor: Tony Robillard | Received 13 December 2022 | Accepted 13 November 2023 | Published 11 March 2024 https://zoobank.org/99977F28-A7D9-4632-BD7C-757B7E047AF4 Citation: Srygley RB, Senior LB (2024) Illustrated review of Mormon cricket Anabrus simplex (Tettigoniidae, Tettigoniinae) embryonic development. Journal of Orthoptera Research 33(1): 87-93. https://doi.org/10.3897/jor.33.98763 Abstract Mormon crickets Anabrus simplex Haldeman, 1852 are a pest of crops and rangeland in the western United States, but little is known about their development in the egg stage. Mormon crickets have multiple states at which they may diapause and thus affect population growth. Conse- quently, a series of photographs of Mormon cricket embryonic stages was organized using published research on Old World katydids. Earlier stages were more difficult to distinguish without removing the chorion. How- ever, where possible, features that can be seen through the chorion are indicated with the expectation that these will be useful in characterizing development in living embryos. As with other Orthoptera, the timing of development varied greatly among individuals, but at a minimum, em- bryos filled approximately half the egg in six weeks, whereas they required 12 weeks from oviposition to reach the final stage before their obligate winter diapause. Keywords diapause, environment, hatching, katydid, ontogeny Introduction In the temperate zone, shield-backed katydids (Orthoptera: Ensifera: Tettigoniidae) spend most of their life cycle in the egg. During much of this time, the embryo may be quiescent or in diapause and not growing. Diapause may occur at multiple stages during embryonic development and last for differing amounts of time (Hartley and Warne 1972). As a result, embryonic mor- phogenesis can vary greatly in its temporal pattern both among individuals and species. Due to this temporal variation, external morphology of embryos is a more reliable means than age (i.e., time from oviposition) for classifying embryonic development (Van Horn 1966, Donoughe and Extavour 2016). General embryology of shield-backed katydids was described by Warne (1972). Based on the location of the primordial cells that give tise to the embryo (embryonic primordium) and the movement of the embryo within the egg, Warne (1972) identified four classes of embryonic development in the Tettigoniidae. Having examined Eu- ropean specimens from 10 of the 21 subfamilies, Warne concluded that development was conserved phylogenetically such that each subfamily was represented by only a single developmental class. Relative to other Orthoptera, members of Tettigoniidae remain underrepresented in descriptions of their external embryological morphology. Even though some species, such as the spiny bush crick- et Acanthoplus discoidalis Walker, 1869 (Bradyporinae) and the Mor- mon cricket Anabrus simplex Haldeman, 1852 (Tettigoniinae: Plat- ycleidini), are economically important (Srygley and Jaronski 2019, Mviha et al. 2022), little is known about their embryology due to the difficulty of rearing them in the laboratory. Mormon crickets are flightless katydids of the western United States. They frequently reach densities that result in significant economic impacts on the grazing industry and force livestock selloffs, especially during droughts when forage is scarce (Cowan and Shipman 1947). Mormon cricket out- breaks are characterized by the formation of dense bands of later- instar nymphs or adults moving directionally as a group (Simpson et al. 2006) and serve as a model for nymphal locust behavior on other continents (Buhl et al. 2006, Srygley 2016). Commonly found on rangeland, Mormon cricket bands also migrate into crops where they can cause major damage (Wakeland 1959). Adult females lay eggs individually in soil ca. 2.5 cm beneath the surface. Eggs have a facultative diapause stage that may occur early in development. In that state, the embryonic primordial tissue may remain for multiple growing seasons and winter periods (Sryg- ley 2020a). The nearly fully developed embryo has an obligate dia- pause stage that requires a cold vernalization period to terminate. In the spring when the soil warms, the nearly fully developed em- bryos complete development and the nymphs hatch (Pfadt 2002). Cowan and Shipman (1940) fixed Mormon cricket eggs in cupric-phenol fixative (Petrunkevitch 1933) and photographed them to create a series of embryonic stages of the Mormon cricket. This early study was not published. The utility of creating a se- ries of photographs of external morphology of the embryo and Copyright Robert B. Srygley & Laura B. Senior. This is an open access article distributed under the terms of the CCO Public Domain Dedication. JOURNAL OF ORTHOPTERA RESEARCH 2024, 33(1) 88 relating the development to more modern treatises of katydid de- velopment (e.g., Warne 1972, Ingrisch 1984) became evident as we learned more about the multiple states in which quiescence, diapause, or aestivation can occur in A. simplex. Both environmen- tal conditions experienced by the parent (Srygley 2020a) and by their eggs (Srygley 2020b) can prolong the time that the eggs re- main in diapause in the soil (i.e., egg banks). The series of photographs by Cowan and Shipman (1940) in- dicated that the location of the embryonic primordium and move- ment of the embryo within the egg were similar to that described by Warne (1972) for the dark bush cricket Pholidoptera griseoaptera (De Geer, 1773), which is in the same subfamily as A. simplex. In a subsequent study, Ingrisch (1984) made several changes to the embryonic stages for P. griseoaptera and described in greater detail the embryonic development of the wart-biter Decticus verrucivorus (Linnaeus, 1758) (Tettigoniinae). In this paper, we document stages of embryonic development for A. simplex organized in accordance with Ingrisch (1984). We use features visible without dissection with the expectation that revelation of stages that are clearly defined through the egg shell and those that are less clearly defined will be useful to field and lab biologists who study Mormon cricket development. A consist- ent embryonic staging system is needed in some developmental studies, particularly in cases where the developmental progress of a living embryo is being monitored. If the stage of an embryo can be identified without removing it from its eggshell, by exam- ining its external appearance, the embryo can continue its devel- opment rather than being terminated during the staging process (Donoughe and Extavour 2016). Our technique relies primarily on the examination of undissected embryos and could lead to bet- ter identification of stages without dissection. Materials and methods Mormon cricket male and female 7“ instar nymphs were collected on Paint Rock Road in the Bighorn Mountains, WY (44°27'52"N, 107°27'33"W, 2653 m.a.s.l..) on July 9, 2015 and brought to the lab in Sidney, Montana. Females were set up in ny- lon mating cages (30 x 21 x 21 cm BugDorms, Megaview Science Co., Ltd., Taiwan) on the day that they molted to adult. If an adult male was available, it too was added to the cage on the same day. Otherwise, a male was added following eclosion. Mating pairs were placed in either long-day (15:9 h day:night) or short-day (12:12 h day:night) treatments with the temperature cycle—warming to 30°C during the day and cooling to 15°C at night—the same in both chambers (Srygley 2020a). They had continuous access to equal parts by volume of hulled sunflower seeds, mixed wild bird seed, dried tropical fish flakes, and half and half wheat germ and wheat bran as well as ad lib access to water in a stoppered bot- tle with a cotton wick. Fresh organic Romaine lettuce was offered daily. On the floor of the cage was an aluminum pan (20 x 20 cm) filled with autoclaved sand (2.5 cm deep) for oviposition. To col- lect the eggs, the sand was sieved weekly. Combined eggs from all mating pairs were evenly divided into two sets of 12 groups (Table 1). Each group of eggs was buried approximately 1.3 cm in sand moistened to 25% saturation with water in a 265 ml cup with a lid. One set was placed into an Associated Environmental Systems (AES) chamber (Acton, Massachusetts, model SD-505) at the beginning of a 12-week variable cycle:variable thermoperiod (VC:VT) temperature profile that is the best currently available for Mormon cricket egg development, diapause, and hatching (see Srygley and Senior 2018, suppl. table S2). The second set was R.B. SRYGLEY AND L.B. SENIOR Table 1. Parental treatments and incubation of eggs preserved for observation of embryonic development. P ] Population, sents Incubation Neggs N embryos photoperiod, : Year environment preserved portrayed temperature Wy, 2015 Tsoi h; 30s1b"C VEN 169 4 Wy, 2015 15:9: hes0ss2G* CeCr 104 9 Wy, 2015 INES AS nies | a no ta VC:VT 156 1 Wy, 2015 2212 nas0elan Gc OE 65 2. Wy, 2018 15 :95h BO152E VGNVT 195 4 OR, 2018 15:9 hy. 30s koe, VC:VT 365 Ds *Thermoperiod was the same although photoperiod varied (Srygley 2020a). **Incubation environments after Srygley and Senior (2018). placed into an AES chamber running a constant daily temperature cycle and constant thermoperiod (CC:CT, Srygley and Senior 2018, suppl. table S1) that cycled between a maximum of 30°C during the day and a minimum of 15°C at night for 10 weeks followed by a two week cooling period. Embryos incubated in CC:CT diapause more intensely and require 18 weeks in subzero temperatures to hatch relative to those incubated in VC:VT, which require only 6 weeks (Srygley and Senior 2018). In 2018, additional A. simplex were collected in the field and set up in mating pairs for egg collection. Males and females were collected from Cedar Spring Road and from Montague Road near Arlington OR on May 28, 2018 (coordinates for Arlington: 44°40'39"N, 120°12'41"W, 87 m) and sent to the lab in Sid- ney. Others were reared in cages in the Bighorn Mountains, WY (44°49'35"N, 107°49'41"W, 2773 m) and brought to the lab on August 28, 2018. An adult male and female from the same location comprised a mating pair. Mating pairs were treated the same as those collected in 2015 except only the long day photoperiod (15:9 h) was used. Moreover, to provide a more precise starting time for embryonic development, the sand in each mating cage was sifted daily and the eggs recovered were sorted into 12 groups and set up simultaneously in the 12-week VC:VT temperature profile. In both years, for each population and incubation treatment, one cup of eggs in sand was removed from the environmental chamber weekly for 12 consecutive weeks following the date of sifting. Thus, in total, for each population and incubation treat- ment, 12 groups of eggs were removed: one group at the end of each week for each of the 12 weeks in the egg development profile. Immediately upon removal, the eggs were placed in a refrigera- tor at 4°C to minimize further development. As soon as possible thereafter, the eggs were sifted out of the sand and then cleared and fixed using a slightly modified version of the Hogan (1959) protocol. The eggs were soaked for 30 min in distilled water at room temperature and any clinging sand particles were gently re- moved. The moist eggs were transferred to a scintillation vial and soaked for 30-45 minutes in a 2:2:1 by volume mixture of glacial acetic acid, chloroform, and absolute ethanol at 37°C. After soak- ing, they were transferred to 1:1 by volume solution of glycerol and 70% ethanol for storage. In addition to stabilizing the tissues, the fixation process increased the transparency of the chorion (Hogan 1959). For review and photography of the egg stages, the eggs were removed from the storage solution and reviewed using a ster- eomicroscope (Leica model M205C), a camera (Leica model DMC 4500), imaging software (Leica Application Suite version 14.13.10), and dark field. For each stage, an image was selected that best represented the characters of a described stage and clearly JOURNAL OF ORTHOPTERA RESEARCH 2024, 33(1) R.B. SRYGLEY AND L.B. SENIOR differentiated the stage from the subsequent and preceding stages in the developmental series. For some stages, the embryo was pho- tographed from more than one perspective, and for some stages, the chorion was removed to better display the characters. Embryonic development of Tettigoniidae is separated into seven phases based on orientation and movement of the embryo. Within each phase, stages of development are differentiated mor- phologically. Phase 0, which includes the first three stages of blas- toderm formation, is not included in this study because it was not visible with our methods nor those of Warne (1972) or Ingrisch (1984). Those authors follow Chapman and Whitham (1968) and separate this phase into Stage 1: No blastoderm; Stage 2: Blas- toderm covers half the yolk, and Stage 3: Blastoderm covers the whole yolk. For orientation, micropyles are ventrally located near the posterior end of the tettigoniid egg (Warne 1972). Results and discussion Phase and stage descriptions.—Phase I: Formation and differentia- tion of the embryonic primordium (Fig. 1). Stage 4: Embryonic primordium circular, appearing as a disc of tissue when the broad side of the disc is oriented facing the viewer (see also, Suppl. material 2: fig. S1). The disc is difficult or impossible to discern when viewed in other orientations. The embryonic primordium lies on the yolk at the posterior end of the egg (slightly off-center). Cowan and Shipman (1940) portray the embryonic primordium in their Fig. 1 (see Table 1). Stage 5: Growth has begun, and the embryonic primordium has become pear shaped and lies on the yolk at the posterior end. A 89 Stage 6: Differentiation into protocephalic (i.e., head) and protocormic (i.e., thoracic and abdominal) regions. Protocormic region begins to elongate but still quite short, although clearly more defined than in Stage 5. The stodomodeum (rudimentary mouth) appears (Ingrisch 1984) but is difficult to see through the chorion. The embryo lies with the anterior end toward the poste- rior end of the egg (i.e., antero-posterior orientation). Stage 6 transitioning to Phase II (Anatrepsis) Stage 7: Segmen- tation, which is difficult to discern in these images, is developing in the gnathal and thoracic regions whereas abdominal segmenta- tion has not begun. The protocorm is still relatively short, while the protocephalon has widened (see also Suppl. material 2: fig. S2). Stage 7 marks the start of Anatrepsis. Phase II: Anatrepsis (Fig. 2) Movement away from the poste- rior end of the egg. Movement is slight relative to other Orthoptera (Warne 1972) and may be neglected altogether (Ingrisch 1984). Anatrepsis is a brief phase in the Tettigoniinae (Warne 1972). Stages 7 to 8: By the end of Stage 7, segmentation of the tho- racic region is complete. The head of the embryo is at the posterior end of yolk, bending slightly backwards. As the segmentation pro- ceeds into the abdomen, the embryo is moving from Stage 7 into Stage 8. The protocorm has elongated, while the protocephalon is somewhat broadened. Limb buds are not visible. Stage 9: Abdominal segmentation is proceeding. At Stage 9, the abdomen is more than half segmented. The abdomen is notice- ably longer now but not yet full length. The thoracic and gnathal limb buds (i.e, rudimentary legs and mouth parts, respectively) now protrude (Suppl. material 2: fig. $3). The thoracic append- ages become larger than the gnathal appendages but difficult to Fig. 1. Phase I: Formation and differentiation of the embryonic primordium. Images show the posterior end of the egg only: A. Stage 4; B. Stage 5; C. Stage 6; and D. Stage 6 transitioning to Stage 7. Scale bars: 2 mm. Fig. 2. Phase II: Anatrepsis. Images show the posterior end of the egg: A. Stage 7 to 8; B. Stage 9; C. Stage 10; D. Stage 11 with micropyles clearly visible. Scale bars: 2 mm. JOURNAL OF ORTHOPTERA IRESEARCH 2024, 33(1) 90 see through the chorion. The anterior end of the embryo remains at the posterior end of yolk, bending slightly backwards. Stages 10: At Stage 10, the proctodeum (i.e., rudimentary anus) is just visible and the abdomen has become completely segmented. Thoracic appendages oriented approximately parallel to the body (In- grisch 1984) or outwards (Warne 1972) (Suppl. material 2: fig. S4). Stage 11: Abdomen completely segmented and full length (Suppl. material 2: fig. $5). The proctodeum is clearly visible. The legs point outwards at right angles to the body. The appendages begin segmentation at this stage; however, this aspect of develop- ment is not visible in these images. The tip of abdomen curls for- ward. The head of the embryo remains at posterior end of yolk, bending backwards. Anatomic details are more visible in the spec- imen with chorion removed (Suppl. material 2: fig. S6). Phase III: Mesentrepsis (Fig. 3) Embryo is stationary (Warne 1972). Stage 12: The tip of the abdomen bends over the ventral side of the embryo (Suppl. material 2: fig. S7). The thoracic append- ages are now directed toward the midline of the body, rather than extending outward as they were previously. The caput is broad and remains bent back slightly. The embryo has not yet begun to broaden (also see dissected specimen, Suppl. material 2: fig. S8). Stage 13: The embryo has begun to broaden conspicuously. The tip of the abdomen, also now noticeably widened, is curled under towards the ventral side (Suppl. material 2: fig. S9). Stage 14: The entire embryo has further broadened with re- spect to the previous stage (Suppl. material 2: fig. S10). The tip of R.B. SRYGLEY AND L.B. SENIOR the abdomen still bends over the ventral side. The antennae reach well past the palpus maxillaris, which is the length of the anten- nae in Stage 13. In the dissected specimen (Suppl. material 2: fig. S11), the antennae extend to the second pair of developing pedes (thoracic limbs). Phase IV: Katatrepsis (Fig. 4) Remaining on the surface of the yolk, the embryo moves around the yolk’s posterior end, reversing the embryo’s orientation and positioning the head towards the anterior end of the egg. Stage 15: The head bends back over the end of the yolk, being at approximately right angles to the body. The embryo is begin- ning to move around the pole of the yolk, initiating the reversal of its antero-posterior orientation (Suppl. material 2: fig. $12). Stage 16: The embryo curves around the end of the yolk, with the head and thorax arching as the embryo undergoes katatrep- sis. At the posterior end of the yolk, a clear fluid filled space has formed from the uptake of water, which occurs before katatrepsis (Warne 1972). The eyespots are visible, although faint (Suppl. ma- terial 2: fig. $13). Stage 17: Only the very tip of the abdomen remains bent back under the yolk as the head is now oriented toward the anterior end of the egg. The ventral side of the embryo is apparent along what is now the opposite side of the egg from where it was situated before katatrepsis. The eyes are reddish pigmented and visible (Suppl. material 2: fig. S14). Stage 18: Katatrepsis is complete, as the embryo has fin- ished rounding the posterior pole. The tip of the abdomen has Fig. 3. Phase III: Mesentrepsis. Images left to right showing the posterior end of the egg: A. Stage 12; B. Stage 13; C. Stage 14; D. Lateral view of same stage 14 embryo. Scale bars: 2 mm. Fig. 4. Phase IV: Katatrepsis. A. Stage 15, lateral view with anterior end of embryo to the left; B. Stage 15, dorso-lateral view with cho- rion removed; C. Stage 16, lateral view with anterior end of embryo to the right; D. Stage 17, lateral view; E. Stage 18, ventral view showing its length relative to the whole egg. Scale bars: 2 mm. JOURNAL OF ORTHOPTERA RESEARCH 2024, 33(1) R.B. SRYGLEY AND L.B. SENIOR 91 Fig. 5. Phase V: Dorsal closure A. Stage 19, ventral view; B. Stage 20, ventral view; C. Stage 21, lateral view; D. Stage 21 to 22, ventral view; Phase VI: Completion E. Stage 23, ventral view; F. Stage 23, lateral view; G. Stage 24, ventral view. Scale bars: 2 mm. straightened out. The developing legs, antennae, and mouth- parts are more conspicuous. The darkened eyespots are readily visible, having a reddish pigmentation and an angled ovoid ap- pearance (Suppl. material 2: fig. $15). The top of the head is shaped like two blunt prongs. The embryo is less than half the length of the yolk. Phase V: Dorsal closure (Fig. 5) The body grows dorsally around the yolk to envelop it. Stage 19: The embryo is half the length of the yolk (Suppl. material 2: fig. S16). The eyespots are larger, more rounded, and generally darker than previously. The top of the head retains the blunt two-pronged form. The posterior femurs are fairly short, as approximately half of the abdomen extends beyond the joint be- tween the tibia and femur of the hindmost legs. In Stage 19, dorsal closure begins, although this aspect of development is not visible in the image. At mean temperatures greater than 26°C, Mormon crickets arrest development at Stage 19 and apparently aestivate (Srygley 2022). Stage 20: The embryo is three-quarters the length of the yolk (Suppl. material 2: fig. $17). While the embryo has elongated, the relative length of the legs has not changed much compared to Stage 19, with approximately half the length of the abdomen projecting beyond the tips of the folded hindmost appendages. The shape of the head has rounded out somewhat, with the two- pronged shape becoming less pronounced. The reddish-brown eyespots are conspicuous. Stage 21: With only a small plug of yolk protruding beyond the head (Suppl. material 2: fig. $18), the embryo almost completely fills the egg. The legs remain somewhat short, covering approxi- mately half the length of the abdomen and having not elongated much compared to Stage 20. Stages 21 to 22: Only a small plug of yolk protruding beyond the head is still visible, and the legs are shorter than in Stage 23. While dorsal closure completes in Stage 22, this is not readily vis- ible from the ventral view. The Stage 21 to 22 embryo has not yet shown any pigmentation of the appendages or head. Phase VI: Completion. Stage 23: The embryo is the full length of the egg. The posterior femurs have become longer, are of me- dium length, and cover more than half of the abdomen (Suppl. material 2: fig. S19). The eyes are larger and dark. There is some pigmentation in the caput and in the pedes. Stage 24: The embryo is the full length of the egg, and the hindmost femurs are long, extending most of the way but not completely to the tip of the abdomen (Fig. 7). The pigmentation of the legs and tarsal spines and head is much advanced. As with Decticus verrucivorus (Ingrisch 1984), the obligate diapause over winter, which is known as embryonic diapause, tends to occur in Stage 23 or 24 for Mormon crickets. Stage 25: Final developmental stage that occurs after embry- onic diapause and prior to hatching (Fig. 7). Timing of the embryonic stages.—The developmental stages are in successive order, and so for an individual embryo, the minimum time to reach Stage n+1 cannot be less than the time to reach Stage n. However, this is not necessarily true for the different embryos portrayed (Figs 1-5). To obtain a rough estimate of the time to reach each stage, the embryos in the photographic images were graphed with their age from oviposition (Fig. 6). The initial stages can develop very quickly. For example, an embryo developed to Age of egg (days) 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Stage Fig. 6. The minimum age of each embryo from day of oviposition (maximum age indicated by error bar). Embryos that were between two stages (e.g., Stage 6 to 7) were given a value half-way between the two stages (e.g., 6.5). Closed circles: embryos incubated at a daily 12:12 h cycle of 30:15°C; open circles: embryos incubated at 30:15°C for the initial 35 days and then the daily maximum and minimum temperatures were varied each week thereafter. Inset: embryos in various stages of development in week 8. JOURNAL OF ORTHOPTERA RESEARCH 2024, 33(1) 92 Phase VI. } Be Stage 25 (chorion removed) ad Fig. 7. Phase VI: Completion. Ventral view of stage 25 embryo with chorion and membrane removed. R.B. SRYGLEY AND L.B. SENIOR Stage 6 within a week or two after oviposition. Other embryos took longer to reach earlier stages, such as two embryos that reached Stage 4 and Stage 5 within 28-35 days and an embryo that reached Stage 6 in 54 days. As in other Orthoptera (Van Horn 1966, Ingrisch 1984, Donoughe and Extavour 2016), develop- mental timing can be highly variable. Two weeks after oviposition, an embryo had reached Stage 11. The later stages took longer to complete. For examples, it took four weeks for an embryo to reach Stage 17 and six weeks for a specimen to reach Stage 19. Stage 23, the beginning of the Completion phase, was reached in nine weeks, and Stage 24 in about 12 weeks. Cowan and Shipman (1940) are credited with the first photographic series of katydid embryology, which they organized so that they could more precisely characterize the development of Mormon crickets in nature. To make it easier to draw comparisons with the literature that came later, we have attempted to reflect their series considering the stages of morphological embryogenesis that Warne (1972) and Ingrisch described (SuppI. material 1). For embryos in the stages before katatrepsis, it is difficult to see details necessary to separate them. Many more photographs are made of stages during katatrepsis and later, which suggests that Cowan and Shipman may have organized their photographs on a temporal scale in addition to a scale of external morphology. Organization by a temporal series is also evident in the occasional appearance of a figure that appears to be out of order when organized by morphogenesis (e.g., C&S 12b is Stage 19, but C&S 13a is Stage 18 in Suppl. material 1). To summarize, we have organized a series of photographs of Mormon cricket embryos by their external morphology in accordance with Ingrisch (1984). This photographic series will prove valuable for understanding the proximate conditions by which development is arrested at different embryonic stages. The ability to predict outbreaks of Mormon crickets depends in part on our understanding the proximate and ultimate causes of their embryonic development. Acknowledgments We thank April Aamodt for collecting Mormon crickets in Oregon, D. Branson for lending the dissecting scope used to photograph the embryos, and FE. Vencl for comments on a draft of the manuscript. 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Journal of Morphology 120: 83-114. https://doi.org/10.1002/ jmor.1051200105 Van 93 Walker F (1869) Catalogue of the Specimens of Dermaptera Saltatoria in the Collection of the British Museum 2: 230. Warne AC (1972) Embryonic development and the systematics of the Tetti- goniidae (Orthoptera: Saltatoria). International Journal of Insect Mor- phology and Embryology 1: 267-287. https://doi.org/10.1016/0020- 7322(72)90034-7 Wakeland C (1959) Mormon crickets in North America. Technical Bull. 1202, U.S. Department of Agriculture, Washington, D.C. Supplementary material 1 Author: Robert B. Srygley, Laura B. Senior Data type: pdf Explanation note: Comparison of developmental stages of Ingrisch (1984) and Warne (1972) and the approximate equivalent of the stages illustrated in Cowan and Shipman (1940). Copyright notice: This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/ odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited. Link: https://doi.org/10.3897/jor.33.98763.suppl1 Supplementary material 2 Author: Robert B. Srygley, Laura B. Senior Data type: pdf Explanation note: fig. S1. Stage 4 circular disc of cells. fig. $2. Stage 6-7 with key characters labeled. fig. $3. Stage 9 with key characters labeled. fig. $4. Stage 10 with key characters labeled. fig. S5. Stage 11 with key characters labeled. The micropyles, clearly visible in this photo, are also indicated. fig. S6. Stage 11 with chorion removed. fig. $7. Stage 12 with key charac- ters labeled. fig. S8. Stage 12 with chorion removed. fig. S9. Stage 13 with key characters labeled. fig. $10. Stage 14 with key characters labeled. fig. $11. Stage 14 with chorion removed and key characters labeled. fig. $12. Stage 15 with key charac- ters labeled. fig. S13. Stage 16 with key characters labeled. fig. S14. Stage 17 with lateral (left) and frontal (right) views of the same embryo. fig. $15. Stage 18 with key characters labeled. fig. S16. Stage 19 with key characters labeled. fig. $17. Stage 20 with key characters labeled. fig. $18. Stage 21 with key char- acters labeled. fig. $19. Stage 23 with key characters labeled. fig. S20. Stage 24 with key characters labeled. Copyright notice: This dataset is made available under the Open Database License __(http://opendatacommons.org/licenses/ odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited. Link: https://doi.org/10.3897/jor.33.98763.suppl2 JOURNAL OF ORTHOPTERA RESEARCH 2024, 33(1)