Zoosyst. Evol. 100 (2) 2024, 391-403 | DOI 10.3897/zse.100.121174 > PENSUFT. [fit Gp tuseue ror BERLIN A new species of trout from the K6priicay River, a drainage of Mediterranean Sea, Ttirktye (Salmoniformes, Salmonidae) Fahrettin Kiictik!, Gékhan Kalayci*, Salim Serkan Giiclii!, Miinevver Oral*, Davut Turan? 1 Department of Basic Sciences, Faculty of Egirdir Fisheries, Isparta University of Applied Sciences, Isparta, Turkiye 2 Department of Basic Sciences, Faculty of Fisheries, Recep Tayyip Erdogan University, Rize, Turkiye https://zoobank. org/54F 22169-B8C9-4A E9-87E8-DDSE16A9BDOE Corresponding author: Davut Turan (dvtturan@yahoo.com) Academic editor: Nicolas Hubert # Received 19 February 2024 # Accepted 15 March 2024 @# Published 4 April 2024 Abstract Salmo ekmekciae, new species, 1s described from the Koprticay River, a drainage of Mediterranean Sea. It is distinguished from Salmo species in adjacent water by having 9-10 parr marks on flank; 11—13 scale rows between end of base of adipose-fin and lateral line; 22—24 gill rakers on first gill arch; a shorter distance between adipose-fin and caudal-fin base; a slenderer caudal peduncle; and a slenderer body at adipose-fin origin. According to the Bayesian, and maximum likelihood analyses, Salmo ekmekciae cyt b gene resulted in coherent trees supported by high bootstrap values. Key Words cytochrome b, freshwater fish, salmo, taxonomy Introduction The first known record of Anatolian inland fishes was provided by Abbolt K.E. in the first half of the 19" cen- tury, and later studies were particularly encountered in the Works of Heckel (1843), Boulenger (1896), and Steindachner (1897) (Geldiay and Balik 1999; Cicek et al. 2023). In the subsequent century, Turkish researchers participated in studies aimed at determining the ichthy- ofauna. For example, F. Battalgil described 25 fish spe- cies from Turkish inland waters between 1940-1944. The studies of this researcher were followed by the works of Kuru (1975, 2004) and Geldiay and Balik (1999). Since the beginning of the 21* century, the number of described inland fish species in Turkiye has rapidly increased, sup- ported by detailed morphology and molecular character- istics. Noteworthy contributions to the taxonomy of Ana- tolian freshwater fishes, in particular, can be attributed to the studies of Bogutskaya (1997), Bogutskaya et al. (2000), Turan et al. (2006, 2008, 2017, 2024), Ozulu® and Freyhof (2011), Kuc¢uk et al. (2016, 2017), Turan et al. (2010, 2012, 2020) and Yogurtcuoglu et al. (2022). Salmo trutta has traditionally been acknowledged as a species widely distributed across Europe, extending southward to the Atlas Range (Morocco, Algeria) and eastward to the upper Amu-Darya drainage in Afghani- stan. Despite numerous identified subspecies or distinct species over time, there has been a persistent inclination to dismiss this diversity, asserting a priori that they all fall under a highly variable “species” (Ferguson 1989, 2004; Guinand et al. 2021). This perspective contends that ‘classical’ taxonomy is inadequate for addressing this species. The state of Salmo taxonomy has been sum- marized in detail by Kottelat (1997), with some discus- sion of North African species by Delling and Doadrio (2005) and Balkan ones by Delling (2003), indicating some improvement in the taxonomic situation. Kottelat and Freyhof (2007) provide an overview of available data for European species, tentatively recognizing 29 species, though the status of several populations and nominal species remains unclear. Recent molecular studies have revealed the existence of at least five molecular lineages (Bernatchez et al. 1992; Bernatchez and Osinov 1995; Bernatchez 2001), Copyright Ktictik, F. et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 392 Kucuk, F. et al.: A new species of trout from the Mediterranean Sea drainage, Turkiye yet modern biology has not effectively resolved the taxonomy of the species. Notably, no efforts have been made to correlate the molecular lineages with morpho- logical data, resulting in a wealth of untapped, intrigu- ing data. Although molecular data such as COI, d Loop and Cyb are not fully successful in distinguishing spe- cies, they have long been providing an important source of knowledge regarding Sa/mo lineages. Emerging next generation sequencing technologies seem to offer a much better resolution to better understand Sa/mo taxonomy. Segherloo et al. (2021) reviewed the taxonomic status of some species in Europe and Asia using the next genera- tion DNA sequence method and successfully presented the taxonomic position of most species under investiga- tion. More recently, Turan et al. (2024) put forward the distribution and taxonomic position of S. duhani (Mar- mara and Aegean basin) as well as described a new spe- cies, S. brunoi, from Susurluk (Marmara basin) for the first time using next generation sequencing technologies. Extensive fieldwork and research carried out in Turkiye has demonstrated a significant diversity. A total Table 1. Native Sa/mo species distributed in Turkiye. of eighteen valid species were recorded or identified (Table 1). Of these, 9 species (S. abanticus, S. araxen- sis, S. ardahanensis, S. brunoi, S. coruhensis, S. duhani, S. euphrataeus, S. fahrettini, S. murathani, S. rizeensis) belong to the Danubian lineage, Sa/mo tigridis to the Ti- eris lineage. Other species of which (S. baliki, S. kottelati, S. labecula, S. munzuricus, S. okumusi, S. opimus S. platy- cephalus) belong to Adriatic lineage. (Tortonese 1955; Behnke 1968; Bernatchez and Osinov 1995; SuSnik et al. 2005; Bardakci et al. 2006; Turan et al. 2010, 2011, 2012, 2014a, b, 2017, 2020, 2021, 2022; Ninua et al. 2018; Tur- an and Aksu 2021). We reassessed Mediterranean Salmo populations in Turkiye. In our previous study (Turan et al. 2012), we examined only 4 samples from the Koprticay River. In the study, we compared these samples and new mate- rials from Koprticay River in detail with the samples from the type locality of S. Jabecula. As a result of this comparison, it was concluded that the Kopriicay pop- ulation belongs to a new species, thus was named as S. ekmekciae. Species Type locality Synonyms Coordinates Salmo abanticus Tortonese, 1954 ~—-FFR 3163, 13, 113-222 mm SL; Turkiye: Bolu prov.: Lake Abant Basin None 40°36'47.32'N, 31225056 Salmo araxensis Turan, Kottelat & FFR 3224, 259 mm SL; FFR 3122, 6, 140-250 mm SL; Turkiye: Kars prov.: Sa/mo trutta caspius, Kessler, | 40°51'0.00'N, Kaya, 2022 Kirkpinar Stream, a tributary of Kars Stream, Aras River drainage 1877 Salmo caspius, Kessler, 43°1'0.00°E 1877 Salmo ardahanensis Turan, FFR 3239, 222 mm SL; FFR 1130, 12, 135-253 mm SL; Turkiye: Ardahan Salmo trutta caspius, Kessler, | 41°6'0.00'N, Kottelat & Kaya, 2022 prov.: Stream Toros, Kura River drainage 1877 Salmo caspius, Kessler, — 42°25'60.00"E 1877 Salmo baliki Turan, Aksu, Oral, FFR 3242, 212 mm SL; FFR 3234, 6, 132-276 mm SL; Turkiye: Agri prov.: None 39°45'31.50'N, Kaya & Baycelebi, 2021 Stream Sinek, a tributary of Murat River, Euphrates River drainage 43°27'52.13'E Salmo brunoi Turan, Baycelebi, FFR 3243, 175 mm SL; FFR 3216, 188-153 mm SL; Turkiye, Bursa prov.: None 40°03'13.07'N, Aksu & Oral, 2024 stream Aras, a tributary of Nillfer River, Susurluk River drainage 29°10'20.03°E Salmo chilo Turan, Kottelat & FFR 3054, 190 mm SL; FFR 3055, 23, 65-235 mm SL; Turkiye: Sivas prov.: Sa/mo trutta macrostigma (non 38°34'53.89'N, Engin, 2012 Akdere Stream, Ceyhan River drainage Dumeril, 1858) 36°57'17.62'E Salmo coruhensis Turan, Kottelat © FFR 3036, 291 mm SL; FFR 3037, 10, 90-380 mm SL; Turkiye: Erzurum Sa/mo trutta labrax, Pallas, 1814 40°30'25.20'N, & Engin, 2010 prov.: Pehlivanli Stream, Coruh River drainage 41°29'10.20"E Salmo duhani Turan & Aksu, 2021 FFR 3183, 228 mm SL; FFR 3184, 15, 95-287 mm SL; Turkiye: Canakkale + Sa/mo trutta macrostigma (non 39°45'0.00'N, prov.: Stream Zeytinli, Gonen River drainage Dumeril, 1858) 27°1'1.20°E Salmo euphrataeus Turan, FFR 1219, 195 mm SL; FFR 1220, 24, 80-260 mm SL; Turkiye: Erzurum None 40°13'11.10'N, Kottelat & Engin, 2014 prov.: Kuzgun Stream, a tributary of Karasu Stream, Euphrates River drainage 41°6'18.30°E Salmo fahrettini Turan, Kalayci, FFRO3231, 232 mm SL; FFRO3232, 20, 134-227 mm SL; Turkiye: None 39°41'44.97'N, Bektas, Kaya & Baycelebi, 2020 Erzurum prov.: Stream Omertepesuyu, a tributary of Karasu Stream, 34°56'4.07'°E Euphrates River drainage Salmo kottelati Turan, Dogan, FFR 3180, 205 mm SL; FFR 03181, 21, 98-210 mm SL; Turkiye: Antalya None 36°32'34.85'N, Kaya & Kanyilmaz, 2014 prov.: Alakir Stream, a coastal stream in Mediterranean Sea Basin 30°17'11.39°E Salmo labecula Turan, Kottelat & FFR 3056, 208 mm SL; FFR 3057, 6, 103-237 mm SL; Turkiye: Nigde None 37°51'53.31'N, Engin, 2012 prov.: Ecemis Stream, Seyhan River drainage 35°4'46.37°E Salmo munzuricus Turan, Kottelat FFR 3161, 205 mm SL; FFR 03162, 17, 127-270 mm SL; Turkiye: Tunceli Sa/mo trutta macrostigma (not 39°20'50.00'N, & Kaya, 2017 prov.: Stream Munzur, Euphrates River drainage. Dumeril, 1858) 39°8'3.00°E Salmo murathani Turan, Kottelat FFR 3240, 255 mm SL; FFR 3121, 18, 60-233 mm SL; Turkiye: Kars prov.: Sa/mo trutta caspius, Kessler, | 40°16'60.00'N, & Kaya, 2022 Keklik Stream, a tributary of Kars Stream, Aras River drainage 1877 Salmo caspius, Kessler, | 42°38'60.00"E 1877 Salmo okumusi Turan, Kottelat & FFR 1251, 213 mm SL; FFR 1254, 10, 75-202 mm SL; Turkiye: Malatya None 37°59'51.10'N, Engin, 2014 prov.: Surgu Stream, Euphrates River drainage 37°57'29.90°E Salmo opimus Turan, Kottelat & FFR 3047, 180 mm SL; FFR 3048, 12, 118-180 mm SL; Turkiye: Antalya Sa/mo trutta macrostigma (non 36°45'45.04'N, Engin, 2012 prov.: Alara Stream, a coastal stream in Mediterranean Sea Basin Dumeril, 1858) 32°1,35:02°E Salmo platycephalus Behnke, FFR 972, 7, 145-184 mm SL; Turkiye: Kayseri prov.: Pinarbasi Stream, None 38°24'15.80'N, 1968 Seyhan River drainage 37°27'39.44E Salmo rizeensis Turan, Kottelat & © FFR 3000, 234 mm SL; FFR 3001, 16, 90-220; FFR 3038, 1, 250mm SL; Salmo trutta macrostigma (non 40°35'19.20'N, Engin, 2010 Turkiye: Erzurum prov.: Ovit (Kan) Stream, Coruh River drainage Dumeril, 1858) 40°51'30.00"E Salmo tigridis Turan, Kottelat & FFR 1250, 220 mm SL; FFR 1253, 9, 136-227 mm SL; Turkiye: Van prov.: Salmo trutta macrostigma (non 38°2'27.63'N, Bektas, 2011 Catak Stream, Tigris River drainage Dumeéril, 1858) 43°2'57.29'E zse.pensoft.net Zoosyst. Evol. 100 (2) 2024, 391-403 Material and methods Fish sampling The care of experimental animals was in accordance with the animal welfare laws, guidelines declared by Republic of Turkiye and the policies approved by RTE Universi- ty Local Ethics Committee for experimentations (Permit reference number 2014/72). First, 80mg/L MS222 was performed for anaesthesia. Secondly, fish were collected for faunal surveys and preserved in 5% formaldehyde or 96% ethanol, later stored in 70% ethanol. Surgical proce- dures were only performed for excision of fin clips. Thus, the experimental conditions did not cause severe stress on specimens under investigation. Morphological analyses All measurements were done point to point (never by pro- jections) as specified in Turan et al., (2010) with a dial caliper calibrated to 1 mm. Number of lateral line scale count, standard length, and the length of the caudal pe- duncle were recorded according to Turan et al. (2010). The last two branched rays articulating on a single ptery- giophore in the anal and dorsal fins are counted as “1/2”. Comparative materials used in this study are listed in Tur- an et al. (2010); Turan et al. (2011); Turan et al. (2014a, b); Turan et al. (2017); Turan et al. (2020); and Turan et al. (2022). Comparison material All materials are from Turkiye except Salmo labrax. Salmo abanticus: FFR 3163, 13, 77-272 mm SL; Bolu prov.: outlet of Abant Lake, 40.5737°N, 31.2957°E. Salmo ardahanensis: FFR 3164, 10, 154-217 mm SL; Ardahan prov.: stream Toros, Kura River drainage, 41.1000°N, 42.4333°E—FFR 3107, 4, 156-192: FFR 3167, 2, 155-182 mm SL; Ardahan prov.: stream Alabalik, Kura River drainage, 41.0500°N, 42.3666°E.— FFR 3110, 4, 67-118 mm SL; Ardahan prov.: stream Karaman at Asikzilal, Kura River drain- age, 41.4166°N, 42.6500°E.—FFR 3136, 16, 99-185 mm SL; Ardahan prov.: stream Kinavur at Cataldere, Kura River drainage, 41.1833°N, 42.6000°E. Salmo araxensis: FFR 3114, 12, 116-201 mm SL; Kars prov.: Susuz district Kayalik stream, a tributary of Kars Stream, Aras River drainage, 40.8166°N, 43.1166°E.— FFR 3115, 15, 93-237 mm SL; Kars prov.: Susuz dis- trict: Porsuklu (Ak¢al1) Stream, a tributary of Kars Stream, Aras River drainage, 40.8000°N, 43.1833°E.— FFR 3118, 6, 95-132 mm SL; Kars prov.: Sarikamis district: Boyali Stream, a tributary of Kars Stream, Aras River drainage, 40.4333°N, 42.5666°E—FFR 3144, 16, 87-265 mm SL; Kars prov.: Susuz district: 393 Incilipinar Stream, a tributary of Kars Stream, Aras River drainage, 40.8166°N, 43.0666°E. Salmo brunoi. FFR 3213, 7, 142-195 mm SL:—FFR 3215, 7, 142-195 mm SL; Turkiye, Bursa prov.: stream Delicay at Kestel, 40.1241°N, 29.2737°E— FFR 3211, 18, 93— 180 mm SL; —FFR 3217, 12, 85-153 mm SL; Turkiye, Bursa prov.: stream Ericek at Osmangazi, 40.0426°N, 29.2098°E. Salmo baliki: FFR 3234, 6, 132-276 mm SL; A&ri prov.: stream Sinek a tributary of Murat River at Taslicay, 39.7587°N, 43.4644°E.—FFR 3205, 3, 175-267 mm SL; Agri prov.: a tributary of Murat River, 39.7307°N, 43.4818°E. Salmo chilo: FFR 3055, 23, 65-235 mm SL; Sivas prov.: stream Akdere at Gurtin, Ceyhan River drainage, 38.6088°N, 36.8962°E. Salmo coruhensis: FFR 3004, 16, 95-240 mm SL; Art- vin prov.: stream Osmaniye at Karaosmaniye village, 41.4689°N, 41.5105°E—FFR 3011, 11, 90-189 mm SL; Artvin prov.: stream Hopa at Cavuslu village, 41.4509°N, 41.7001°E—FFR 3021, 25, 90-520 mm SL; Rize prov.: stream Firtina at Cat village, 40.8653°N, 40.9311°E—FFR 3022, 9,95-228 mm SL; Rize prov.: stream Kendirli at Kalkandere Dis- trict on road to Kendirli village, lyidere drainage, 40.9373°N, 40.4320°E—FFR 3023, 13, 120-450 mm SL; Rize prov.: stream Iyidere (Ikizdere) at Gtineyce, 40.8219°N, 40.4765°E.—FFR 3024, 13, 115-330 mm SL; Artvin prov.: stream Dortkilise at Tekkale village, Coruh River, 40.7877°N, 41.4946°E—FFR 3025, 13, 80-550 mm SL; Erzurum prov.: stream Cayir- basi (Kirik) at Kirik village, Coruh River, 40.2904°N, 40.8097°E—FFR 3026, 6, 160-290 mm SL; Erzurum prov.: stream Bityuk at Buyukkoy village, Coruh River, 40.4452°N, 40.8513°E—FFR 3027, 6, 130-420 mm SL; Rize prov.: stream Velikéy at Velikoy village, 41.0332°N, 40.6145°E—FFR 3029, 6, 130-220 mm SL; Rize prov.: stream Bozukkale at Bozukkale village, 41.0543°N, 40.6297°E—FFR 3030, 6, 80-170 mm SL; Rize prov.: stream Caglayan at Caglayan district, 40.9230°N, 40.4452°E—FFR 3031, 6, 190-265 mm SL; Bayburt prov.: stream Olcer at Olcer village, Coruh River, 40.5147°N, 40.5609°E—FFR 3032, 16, 70-310 mm SL; Rize prov.: stream Sogitli at Sogutlt village, about 5 km west of Cayeli, 41.0659°N, 40.6526°E. FFR 3033, 16, 110-210 mm SL; Bayburt prov.: stream Kurtbogazi at Kurtbogaz: village, Coruh River, 40.1883°N, 40.5033°E—FFR 3034, 16, 70-210 mm SL; Gumishane prov.: stream Harsit at Ya&murdere, 40.5746°N, 39.8645°E—FFR 3035, 9, 160-450 mm SL; Sivas prov.: stream Gemin at Camili, Yesilirmak River drainage, 38.0536°N, 40.0619°E—FFR 3037, 10, 90-380 mm SL; Erzurum prov.: stream Pehlivanli at Pehlivanli village, tributary of Tortum, Coruh River, 40.5176°N, 41.4780°E—FFR 3041, 10, 115-250 mm SL; Trabzon prov.: stream Solakl at Taskiran village 40.6722°N, 40.2568°E—FFR 3042, 6, 95-117 mm zse.pensoft.net 394 Kucuk, F. et al.: A new species of trout from the Mediterranean Sea drainage, Turkiye SL; Rize prov.: stream Saraykoy at Saraykoy village, 41.0190°N, 40.3807°E—FFR 3043, 5, 130-229 mm SL; Artvin prov.: stream Barhal at Sarigol village, Coruh River, 40.9744°N, 41.4184°E—FFR 3043, 9, 110-223 mm SL; Rize prov.: stream Derepazari1 at Derepazari 41.0237°N, 40.4293°E—FFR 3044, 6, 100-250 mm SL; Rize prov.: stream lyidere at Iyidere, 40.9676°N, 40.3778°E—FFR 3045, 7, 150-450 mm SL; Rize prov.: stream Firtina at Camlihemsin, 41.0517°N, 41.0032°E—FFR3046, 5, 10-280 mm SL; Rize prov.: stream Limankoy at Limankoy village, 41.0714°N, 40.7121°E. Salmo duhani: FFR 3184, 15, 95-287 mm SL; Canakkale prov.: stream Zeytinli about 9 km east of Kazdag1 Na- tional Park, 39.750°N, 27.017°E. —FFR 3185, 14, 85— 170mm SL; Canakkale prov.: stream Zeytinli, 39.749°N, 27.015°E—FFR 3186, 12, 108-160 mm SL; Canak- kale prov.: stream Zeytinli 39.759°N, 27.021°E—FFR 3194, 10, 62-122 mm SL; Canakkale prov.: stream Ko- cacayl, 12 km west of Kalkim, 39.804°N, 27.071°E.— FFR 3195, 15, 93-275 mm SL; Canakkale prov.: stream Kocagcay at Yenice, 39.817°N, 27.099°E. Salmo euphrataeus: FFR 1220, 24, 80-260 mm SL; Erzurum prov.: stream Kuzgun, a tributary of Kara- su Stream, Euphrates River drainage, 40.2198°N, 41.1051°E—FFR 1255, 25, 88—230 mm SL; Erzurum prov.: stream Senyurt at Senyurt, a tributary of Karasu Stream, Euphrates River, 40.1830°N, 41.5037°E— FFR 1223, 5, 122—222 mm SL; Erzurum prov.: stream Sirli, a tributary of Karasu Stream, Euphrates River, 40.2183°N, 41.1010°E—FFR 1269, 8, 117-198 mm SL; Erzurum prov.: stream Kuzgun, Euphrates River, 40.2198°N, 41.1050°E. Salmo fahrettini: FFR 3232, 20, 134-227 mm SL; Er- zurum prov.: stream Omertepesuyu at Palandoken 39.7958°N, 40.9444°E. —FFR 3233, 5, 126-194 mm SL; Erzurum prov.: stream Tekke at Paland6oken, 39. 8197°N, 41.1516°E. Salmo kottelati: FFR 3181, 21, 98-210 mm SL; Anta- lya prov.: stream Alakir at Altinyaka, 36.5608°N, 30.3428°E—FFR 3182, 16, 98-176 mm SL; An- talya prov.: stream Alakir at Altinyaka, 36.5608°N, 30.3428°E. Salmo labecula: FFR 3057, 4, 103—237 mm SL; Nigde prov.: stream Ecemis at Camardi, Seyhan River drain- age, 37.8253°N, 34.9902°E.—FFR 3058, 5, 142-241 mm SL; Isparta prov.: stream Kartoz at AsaS1yaylabel, Kopricgay drainage, 37.5532°N, 31.3070°E—FFR 3059, 5, 140-184 mm SL; Antalya prov.: stream Zindan at Aksu, Koprticay drainage, 37.8064°N, 31.0734°E. Salmo munzuricus: FFR 3162, 17, 127-270 mm SL; Tunceli prov.: stream Munzur at Koyungoli, 39.3472°N, 39.1341°E—FFR 3147, 8, 146-320 mm SL; stream Munzur at Koyungolt, 39.3461°N, 39.1316°E. Salmo murathani: FFR 3121, 18, 60-233 mm SL; Kars prov.: Keklik stream [a tributary of Kars stream], Sarikamis district, Aras River drainage, 40.2833°N, 42.6500°E.—FFR3117, 22, 95-192 mm SL; FFR 3113, 17, 91-206; Kars prov.: Keklik stream [a tributary of zse.pensoft.net Kars stream] Sarikamis district, Aras River drainage, 40.2500°N, 42.6666°E—FFR 3120, 10, 69-163 mm SL, Kars prov.: Maksutcuk Stream [a tributary of Kars stream], Aras River drainage, 40.5333°N, 42.8666°E. — FFR 3108, 14, 90-186 mm SL; Ardahan prov.: Cildir Lake, Aras River drainage, 41.0500°N, 43.3166°E.— FFR 3228, 23, 95-241 mm SL; Kars prov.: Arpacay stream [a tributary of Kars stream] Arpacay district, Aras River drainage, 40.9000°N, 43.1666°E.—FFR 3229, 8, 110-156 mm SL; Kars prov.: Keklik Stream [a tributary of Kars stream] Sarikamis District, Aras River drainage, 40.2833°N, 42.6500°E. Salmo okumusi: FFR 1254, 10, 75-202 mm SL; Malatya prov.: stream Sutrgt, Euphrates River drainage, 37.9975°N, 37.9583°E.—FFR 125, 10, 129-169 mm SL; Sivas prov.: stream Gokpinar, a tributary of Tohma Stream, Euphrates River, 38.6600°N, 37.3089°E.— FFR 1256, 10, 68—280 mm SL; Sivas prov.: stream Gokpinar, Euphrates River, 38.6600°N, 37.3089°E.— FFR 124, 2, 149-175 mm SL; Kahramanmaras prov.: stream Goksu 4 km north of Dizbag, Euphrates River, 37.8331°N, 37.4756°E. Salmo opimus: FFR 3048, 12, 118-180 mm SL; Anta- lya prov.: stream Alara at Gundogmus, 36.7921°N, 31.9749°E —FFR 3049, 20, 115-186; Kahraman- maras prov.: stream Godcutksu at Komurkoy, Ceyhan River drainage, 38.1447°N, 36.5630°E.—FFR 3050, 4, 175-210 mm SL; Kahramanmaras prov.: drain- age of stream Tekir at Tekir, Ceyhan River drainage, 37.8767°N, 36.6058°E.—FFR 3051, 9, 90-300 mm SL; Kahramanmaras prov.: stream Firniz at Fuirniz, Ceyhan River drainage, 37.7591°N, 36.6983°E. Salmo platycephalus: FFR 972, 7, 145-184 mm SL; Kayseri prov.: Pinarbas1 Stream at Pinarbasi district, Seyhan River drainage, 38.4043°N, 37.4609°E —FFR 1260, 10, 137-237 mm SL; Kayseri prov.: Pinarbas1 Stream at Pinarbas: district, Seyhan River drainage, 38.4044°N, 37.4609°E. Salmo rizeensis: FFR 3001, 15, 90-220 mm SL; Erzurum prov.: stream Ovit (2) [Kan] at Ovit mountain, Coruh River, 40.5887°N, 40.8583°E—FFR 3002, 10, 114— 245 mm SL; Trabzon prov.: stream Degirmen at Cosan- dere village, 40.7512°N, 39.5908°E—FFR 3003, 12, 112-230 mm SL; Trabzon prov.: stream Solakli at Demirkapi village, 40.7586°N, 40.5913°E—FFR 3005, 13, 111-220 mm SL; Rize prov.: stream CaSlayan at Gurctiduzti plateau, 41.1905°N, 41.3086°E—FFR 3006, 18, 95-226 mm SL; Rize prov.: stream Sehit- lik at Sehitlik village, 41.1407°N, 40.9828°E—FFR 3007, 12, 90-118 mm SL; Rize prov.: stream Cayeli at Kaptanpasa village, 40.958°N, 40.7794°E—FFR 3008, 18, 91-198 mm SL; Rize prov.: stream Firti- na at Tunca village, 41.1259°N, 41.1310°E—FFR 3009, 10, 110-240 mm SL; Rize prov.: stream Tasli- dere at Pasacur village, 40.8837°N, 40.5796°E—FFR 3010, 9, 110-240 mm SL; Rize prov.: stream Tasli- dere at Kangel village, 40.9453°N, 40.6642°E—FFR 3011, 7, 100-180 mm SL; Rize prov.: stream Eren- ler at Erenler village, 41.0914°N, 40.8298°E—FFR Zoosyst. Evol. 100 (2) 2024, 391-403 3012, 7, 88—237 mm SL; Artvin prov.: stream Dort- kilise at Tekkale Village, Coruh River, 40.7800°N, 41.5098°E.—FFR 3013, 12, 75-167 mm SL; Artvin prov.: Ciftekoprii’ Stream at Cankurtaran mountain, Coruh River, 41.3844°N, 41.5691°E—FFR 3014, 7, 112-201 mm SL; Artvin prov.: stream Kapisre at Kuctkkéy village, 41.2753°N, 41.3755°E—FFR 3015, 9, 113-228 mm SL; Bayburt prov.: stream Kop at Kop Mountain, Coruh River, 40.0654°N, 40.4331°E—FFR 3016, 9, 113-221 mm SL; Erzurum prov.: stream Yagli at Yagli village, Coruh River, 40.3643°N, 41.0728°E.—FFR 3017, 12, 112-223 mm SL; Erzurum prov.: stream Buytik at Bityiikdere pla- teau, Coruh River drainage, 40.5698°N, 40.7140°E.— FFR 3018, 16, 145-224 mm SL; Gumiushane prov.: stream Akbulak at Akbulak village, Yesilirmak Ruiv- er drainage, 40.281462°N, 39.0896°E—FFR 3019, 10, 122-221 mm SL; Kiutahya prov.: stream Sefakoy at Domanic, Sakarya River drainage, 39.8426°N, 29.6706°E.—FFR 3020, 10, 111-119 mm SL; Kiita- hya prov.: Catalalic Stream at Domanic, Sakarya River, 39.8600°N, 29.6291°E—FFR 3036, 10, 130-170 mm SL; Rize prov.: stream Ikizdere at Anzer plateau, 40.5926°N, 40.5148°E—FFR 3038b, 7, 130-170 mm SL; Rize prov.: stream Ciftekavak at Ortapazar village, 40.9959°N, 40.4851°E—FFR 3039a, 14, 120-200 mm SL; Rize prov.: stream Firtina at Elevit Plateau, 40.8471°N, 41.0151°E—FFR 3038a, 1, 250 mm SL; Erzurum prov.: stream Ovit (2) [Kan] at Ovit mountain, Coruh River, 40.5735°N, 40.8634°E—FFR 3039b, 10, 90-238 mm SL; Rize prov.: stream Ovit at Ovit moun- tain, lyidere drainage, 40.6361°N, 40.8214°E —FFR 3040, 14, 90-190 mm SL; Erzurum prov.: stream Mere- kum at Merekum, Coruh River, 40.5527°N, 41.4592°E. Salmo tigridis: FFR 1253, 9, 136-227 mm SL; Van prov.: stream Catak, Tigris River, 38.0077°N, 43.0652°E. DNA extraction, PCR and Sequencing Total DNA was extracted from fin clips via Hibrigen Ge- nomic DNA isolation kit and DNA quality were checked on 0.8% agarose gel electrophoresis. Mitochondrial cy- tochrome b gene (Cyt 5) (991 bp) was amplified using SsaL14437 (Warheit and Bowman 2008) and StrCBR (Turan et al. 2010) primer pair. PCRs were applied in a 50 uL reaction volume with a T100 thermal cycler (Bio-Rad, Hercules, CA, USA), including 100 ng of DNA, 10X PCR buffer, 3 mM MgCl, 5 wL of 0.5 mM dNTPs mix, | u Taq DNA polymerase (Thermo Scientific Inc.) and 0.5 mM of each primer. PCR amplifications were conducted under the following conditions: initial denaturation 2 min at 95 °C, denaturation 30 s at 95 °C, annealing 30 s at 56 °C, extension 70 s at 72 °C through 35 cycles and a final ex- tension 7 min at 72 °C. The PCR products were run at 1% agarose gel electrophoresis and visualized under the UV Quantum—Capt ST4 system (Vilber Lourmat, France). Pu- rification and sequencing of PCR products were performed by Macrogen Europa Inc. (Amsterdam, Netherlands). 395 Molecular data analysis We have used the newly generated twelve Cyt b sequenc- es from the present study and included an additional 51 specimens from earlier studies deposited to NCBI Gen- Bank (Créte-Lafreniere et al. 2012; Tougard et al. 2018; Turan et al. 2020; Turan et al. 2022). Clustal W algo- rithm (Thompson et al. 1994) in Bioedit v7.2.5 (Hall, 1999) was used to align Cyt b sequences. Sequences were submitted to NCBI GenBank with accession num- bers OR713904—OR713909. Nucleotide substitution model TrN+I+G model: -In= 1781.6420 (Tamura and Nei 1993) was chosen as the best nucleotide substitution model according to the Bayesian information criterion (BIC) in jModeltest v. 0.0.1 (Posada, 2008). Phyloge- netic relationships among species were carried out us- ing maximum likelihood (ML) using MEGA X (Kumar et al. 2018) with 100 bootstrap and Bayesian inference (BI) analysis using MrBayes 3.2 software (Ronquist et al. 2012). BI analysis was run using a Metropolis-coupled Markov chain Monte Carlo (MCMC) algorithm for one million generations in the MrBayes 3.1.2 software (Ron- quist and Huelsenbeck 2003), and the initial 25% of the saved trees sampled in each MCMC run were discarded as burn-in. Salmo ohridanus (JX960763) was selected as an outgroup taxa for all phylogenetic analyses. Pairwise genetic distance estimation among the species was calcu- lated by MEGA X software using the p-distance substitu- tion model (Kimura, 1980). Collection codes IFC-ESUF, Inland Fishes Collection, Faculty of Egirdir Fisheries, Isparta University of Applied Sciences, Isparta; and FFR, Zoology Museum, Faculty of Fisheries, Recep Tayyip Erdogan University, Rize. Results Phylogenetic placement of Salmo ekmekciae The resulting phylogeny indicates that the studied Sal- mo species are divided into six main clades: Adriatic, Danubian, Tigris, Atlantic, Mediterranean and Marmor- atus lineage. Salmo ekmekciae is involved Adriatic lin- eage with S. kottelati, S. chilo, S. labecula, S. munzuri- cus, S. okumusi, S. baliki, S. platycephalus and S. opimus with Marmoratus lineage. Salmo ekmekciae more close- ly related Salmo chilo and Salmo kottelati than to other species included in the analysis (Fig. 1). The Bayesian and Maximum Likelihood analyses of Cyt b gene result- ed in coherent trees supported by high bootstrap values. p distance between species ranged from 0.00% (S. kotte- lati and S.chilo; S. opimus and S. marmoratus, S. duhani and S. brunoi, S. euphrataeus and S. murathani) to 1.6% (S. tigridis and S. araxensis (Suppl. material 1). P dis- tance 1s 0.001% between S. ekmekciae sp nova and its closest relatives S. kottelati and S.chilo. zse.pensoft.net 396 Kucuk, F. et al.: A new species of trout from the Mediterranean Sea drainage, Turkiye Salmo kottelati Alakir stream, Turkiye MT515724 Salmo kottelati Alakir stream, Turkiye MT515724 Salmo kottelati Alakir stream, Turkiye MT515724 Salmo chilo Akdere stream, Turkiye MW366850 Salmo chilo Akdere stream, Turkiye MW366851 Salmo chilo Akdere stream, Tiirkiye MW366852 Salmo ekmekciae Kopricay River, Turkiye OR713904 69/0,90 Salmo ekmekciae K6prigay River, Turkiye OR713905 Salmo ekmekciae K6prigay River, Turkiye OR713906 -/0,98 Salmo ekmekciae KOprigay River, Tirkiye OR713907 Salmo labecula Ecemis stream, Turkiye MW366847 92/097| Salmo labecula Ecemis stream, Turkiye MW366848 Salmo labecula Ecemis stream, Turkiye MW366849 Adriatic Salmo platycephalus Soguksu stream, Turkiye JX960842 lineage Salmo platycephalus Pinarbasi stream, Turkiye MW366844 Salmo platycephalus Pinarbasi stream, Turkiye MW366845 86/1.00 | Salmo okumusi G6kpinar stream, Turkiye MN815915 Salmo okumusi Gékpinar stream, Turkiye MN815915 Salmo okumusi Gokpinar stream, Turkiye MN815915 Salmo munzuricus Munzur stream, Turkiye MW382946 Salmo munzuricus Munzur stream, Turkiye MW382947 Salmo munzuricus Munzur stream, Turkiye MW382948 Salmo baliki Murat River, Turkiye MW366857 Salmo baliki Murat River, Turkiye MW366856 670.941 Salmo baliki Murat River, Tiirkiye MW366858 Salmo opimus Alara stream, Tiirkiye OR713908 64/0.94 76/100 98/0.831| Salmo opimus Alara stream, Tiirkiye OR713909 Marbled Salmo marmoratus Zala River, Slovenia JX960838 lineage Salmo marmoratus Volaja, Slovenia LT617576 99/1.00 Salmo sp1 Corsica, France LT617579 Mediterranean Salmo sp2 Volturno, France LT617578 lineage Salmo fahrettini Omertepesuyu stream, Turkiye MN815913 Salmo fahrettini Omertepesuyu stream, Tiirkiye MN815913 68/0.87 | Salmo fahrettini Omertepesuyu stream, Tiirkiye MN815913 Salmo abanticus Abant Lake, Turkiye MT515716 Salmo duhani Goénen stream, Turkiye PP347761 Salmo duhani Gonen stream, Turkiye PP347762 Salmo duhani G6énen stream, Turkiye PP347763 60/0.87] Salmo brunoii Aras stream, Turkiye PP347764 Salmo brunoii Aras stream, Turkiye PP347765 Salmo brunoii Aras stream, Turkiye PP347766 Paes seh Salmo coruhensis Hisardere stream, Turkiye MT515718 lineage Salmo coruhensis Gayirbasi stream, Turkiye MT515719 88/1.00 Salmo coruhensis Kanli stream, Turkiye MN815912 Salmo rizeensis Kangel stream, Turkiye OR441103 73/0.89 Salmo rizeensis Kangel stream, Turkiye MN815910 Salmo rizeensis Kangel stream, Turkiye OR441102 Salmo euphrataeus Sirli stream, Turkiye MN815911 Salmo euphrataeus Sirli stream, Turkiye MN815911 Salmo euphrataeus Sirli stream, Turkiye MN815911 Salmo euphrataeus Sirli stream, Turkiye MN815911 Salmo murathani Aras River, Turkiye OR441093 Salmo murathani Aras River, Turkiye OR441094 Salmo murathani Aras River, Turkiye OR441092 Salmo araxensis Aras River, Turkiye OR441089 Salmo araxensis Aras River, Turkiye OR441090 Salmo araxensis Aras River, Tirkiye OR441091 Salmo tigridis eaik stream, Tiirkiye MN815916 64/0.93 83/1.00 59/0.88 99/1.00 Tigris lineage Salmo tigridis Gatak stream, Turkiye MN815916 Salmo tigridis Gatak stream, Turkiye MN815916 99/100 | Salmo trutta Corsica, Belle Bone, France LT617533 Atlantic Salmo trutta Sardinia, Flumendosa, Italy LT617536 Salmo trutta Corsica, Belle Bone, France LT617531 Salmo ohridanus Lake Ohrid JX960763 lineage -—________ 0.0050 Figure 1. Maximum likelihood (ML) tree based on mitochondrial cytochrome b gene sequences of Salmo species. Bayesian infer- ence and ML analyses resulted in congruent trees. Bootstrap and posterior probability values are shown above nodes on tree if 50% or higher. zse.pensoft.net Zoosyst. Evol. 100 (2) 2024, 391-403 Morphological differences and comparisons Salmo populations from Kopriticay River are distinguished from the other species of trout recorded from the streams and rivers in Turkish Mediterranean coast (S. /abecula, S. kotte- lati, S. platycephalus, S. opimus and S. chilo) by the follow- ing characters: Salmo populations from Kopriigay River differ from S. /abecula by having fewer parr marks on flank (9-10, vs. 11-12), fewer scale rows between end of base of adipose fin and lateral line (11-13, vs. 14-15), a longer head (27-30% SL, vs. 25-27), a shorter distance between adipose fin and caudal-fin base (13-15% SL, vs. 16-18), a slender- er caudal peduncle (9-10% SL, vs. 10-11) and a slenderer body at adipose-fin origin (13-14% SL, vs. 14-15). Salmo populations from Kopricay River differ from S. kottelati by the general body colour silvery in life (vs. brownish), and no black spots on top of head (vs. more or less presence). Salmo populations from Koprtigay River further differ from S. kottelati in having more gill rakers on first gill arch (22—24, vs. 18-20), a slenderer caudal peduncle (9-10% SL, vs. 10-13), a shorter maxilla in male (8—10% SL, vs. 10-13) and a smaller mouth gape in males (11-13% SL, vs. 13-19). Salmo populations from Koprucay River differ from S. platycephalus by having fewer parr marks along lateral line (9-10, vs. 12-13), the presence of black spots in all size (vs. absent in specimens larger than about 200 mm SL), the head not flattened dorso-ventrally (vs. flattened dorso-ven- trally), a shorter distance between adipose fin and caudal-fin base (13-15% SL, vs. 15-17), a slenderer caudal peduncle (9-10% SL, vs. 11-12) and a slenderer body at adipose-fin origin (13-14% SL, vs. 14-16. Salmo populations from Koprtigay River differ from S. opimus by no red spots on flank in specimens larger than about 160 mm SL, if the red spot present in specimens larger than about 160 mm SL, they almost covered with black dots (vs. presence in all size and not covered with black dots), a slenderer caudal peduncle (9-10% SL, vs. 11-12) and a slenderer body at adipose-fin origin (13—14% SL, vs. 15—17). It differs from S. chilo by the absence of red spots on flank in specimens larger than about 160 mm SL, if the red spot is present in specimens larger than about 160 mm SL, they are almost covered with black dots (vs. presence in all size, and red spots not covered with black dots), fewer parr marks along lateral line (9-10, vs. 11-13), more gill rakers on first gill arch (22—24, vs. 18-21) and a slenderer caudal peduncle (9-10% SL, vs. 11-12). Salmo populations from Kopriigay River are distin- guished from S. baliki, S. okumusi and S. munzuricus by having more gill rakers on first gill arch (22-24, vs. 16- 21), fewer scale rows on lateral line and dorsal-fin origin (21-25, vs. 26-30), fewer scale rows on lateral line and anal-fin origin (16-18, vs. 18—28) and fewer scale rows between origin of the adipose fin and lateral line (11-13, vs. 13-17) and a longer head in males (28-30% SL, vs. 24-27). It further differs from S. okumusi and S. munzuri- cus by having fewer parr marks on flank (9-10, vs. 10-14). Salmo populations from Koprticay River are also dis- tinguished from other species (S. abanticus, S. araxen- sis S. ardahanensis, S. brunoi, S. coruhensis, S. duhani, 397 S. euphrataeus, S. fahrettini, S. munzuricus, S. murathani, S. rizeensis, S. tigridis) by having the presence of four broad dark bands on flank (vs. absent), black spots on body irregularly shaped (vs. roundish), height of parr marks on anterior of the flank 2.5—3.5 times its width (vs. 1.4—2.5), fewer parr marks on flank (9-10, vs. 10-14), more gill rakers on first gill arch (22—24, vs. 16-22, except S. mu- rathani), fewer scale rows lateral line and dorsal-fin ori- gin (21-25, vs. 26-35, except S. araxensis), fewer scale rows lateral line and anal-fin origin (16-18, vs. 18—26, except S. ardahanensis) and fewer scale rows between or- igin of the adipose fin and lateral line (11-13, vs. 13—20). Thus, we describe Salmo populations from Kopriicay River, as a new species, Salmo ekmekciae sp. nov. Salmo ekmekciae sp.nov https://zoobank. org/32FCE 1C6-ABD9-485A-9B37-D825C523447A Type material. Holotype. IFC ESUF 02-0029, holotype, 216mm SL, male; Turkiye: Isparta prov.: Yayla Stream, a drainage of Koprti¢ay River, 37.8115°N, 31.0925°E. Paratypes. IFC-ESUF 02-0022, 6, 70-150 mm SL; same data as holotype; FFR 3058, 4, 141-185 mm SL; Turkiye: Isparta prov.: Kartoz Stream (Kopriicay River drainage), 37.7162°N, 31.1616°E. Diagnosis. Salmo ekmekciae is distinguished from all the species of Salmo in Turkiye and adjacent areas by combination of follow characters: one small black spot in postorbital and suborbital areas, greater than pupil; seven to seventeen black spots on opercle; black spots on body few or numerous, scattered on the back (missing in the pre- dorsal area), a middle portion of flank, sometimes upper and lower halves of the flank. Red spots few, ocellated, organized in two or three irregular longitudinal rows on median part of the body, and half of lower part of the flank; commonly no black spots on flank in specimens larger than 160 mm SL, if red spots present in specimens larger than about 160 mm SL, they are almost covered with black dots; maxilla short and narrow; lateral line with 108—118 scales; 21-25 scale rows between dorsal-fin origin and lateral line; 16—18 scale rows between anal-fin origin and lateral line; 11—13 scale rows between origin of the adipose fin and lat- eral line. 22—24 gill rakers on outer side of first gill arch. Description. The general appearance is shown in Figs 2-4, morphometric data are in Table 2. Body mod- erately deep, compressed laterally, its maximum depth markedly smaller than head length. Dorsal profile slightly convex and ventral profile less convex than the dorsal pro- file. Head somewhat long, upper profile slightly convex in interorbital area, markedly convex in interorbital and on snout. Mouth small, slightly sub-terminal in males, sub-inferior in females. Tip of lower jaw slightly curved upwards, slightly pointed, with a slightly developed pro- cess at symphysis in males larger than 180 mm SL. Max- illa short, not reaching beyond posterior margin of the eye in males and females. Snout short, slightly rounded in males, rounded in females. Adipose fin somewhat large, zse.pensoft.net 398 Kucuk, F. et al.: A new species of trout from the Mediterranean Sea drainage, Turkiye 2S he ees 2 een. 4 to : : Fall ee. ae ee — Figure 4. Salmo ekmekciae, IFC-ESUF 02-0022, paratype, 84 mm SL, juvenile; Turkiye: Kopriigay River. its height 7-9% SL in males and about 7% SL in females, slightly increasing with body size. Largest observed spec- imen 185 mm SL. Lateral line with 108-118 scales; 21—25 scale rows be- tween dorsal-fin origin and lateral line; 16—18 scale rows between anal-fin origin and lateral line; 11—13 scale rows between origin of the adipose fin and lateral line. Dorsal fin with 9-10 branched and 3-4 unbranched rays, its dis- tal margin straight or slightly convex. Pectoral fin with 1 zse.pensoft.net unbranched and 11-13 branched rays, its external mar- gin convex. Pelvic fin with 1 unbranched and 8 branched rays, its external margin slightly convex. Anal fin with 3 unbranched and 8 branched rays, its distal margin straight or slightly convex anteriorly and slightly concave poste- riorly. Caudal fin slightly forked, lobes slightly pointed. 22-24 gill rakers on the outer side of first gill arch. Coloration. In formalin: General coloration of freshly preserved specimens silvery on back and flank, yellowish Zoosyst. Evol. 100 (2) 2024, 391-403 Table 2. Morphometry of Salmo ekmekciae (holotype, IFC-ESUF 02-0029; paratypes FFR 3058, IFC-ESUF 02-0022, n=9. The calculations include the holotype. Number of specimens Holotype Sex male Standard length (mm) 216 In percentage of standard length Head length 25.9 Predorsal length 45.2 Prepelvic length 55.5 Preanal length 75.6 Body depth at dorsalfin origin 20.9 Body depth at dipose-fin origin 14.1 Depth of caudal peduncle 10.2 Length of caudal peduncle 15.0 Distance between adipose- and caudal-fins 13.9 Body width at analfin origin 9.7 Length of dorsal-fin base 15.7 Depth of dorsal-fin 15.6 Length of pectoral-fin 18.3 Length of adipose-fin base 3.8 Depth of adipose-fin 7.1 Length of pelvic-fin 133 Depth of analfin 15.7 Length of analfin base 12.1 Length of upper caudalfin lobe 13.4 Length of median caudal-fin rays 12.2 Length of lower caudaLfin lobe 14.6 Snout length TD Distance between nasal openings 4.1 Eye diameter 4.8 Interorbital width 7.1 Head depth through eye 12.8 Head depth at nape 16.8 Length of maxilla 8.1 Maximum height of maxilla 25 Width of mouth gape 9.3 Length of mouth gape 11.2 male female n=5 n=5 101-185 84-150 Range (mean) Range (mean) 27.5-30.4 (28.7) 27.1-28.0 (27.4) 44.9-50.0 (47.2) 45.4-47.0 (46.3) 53.4-57.3 (55.4) 52.6-54.3 (53.4) 74.3-75.8 (75.2) 73.2-74.8 (73.4) 24.1-27.5 (25.8) 22.3-23.5 (23.0) 13.1-14.4 (13.9) 12.7-13.0 (12.9) 9.2-10.2 (9.9) 9.6-10.1 (9.8) 14.6-18.0 (16.1) 14.4-15.3 (15.0) 14.0-15.3 (14.6) 13.1-14.5 (14.0) 8.7-11.0 (10.2) 9.1-9.3 (9.2) 14.8-19.9 (17.3) 14.6-15.4 (15.0) 14.9-19.8 (18.4) 17.0-17.7 (17.3) 17.3-22.3 (19.8) 18.3-20.9 (19.7) 3.8-4.9 (4.4) 3.8-3.9 (3.9) 7.0-8.7 (7.8) 6.6-7.5 (7.0) 13.1-15.6 (14.8) 13.6-14.8 (14.2) 14.3-18.3 (15.7) 14.2-16.8 (15.3) 9.9-12.9 (11.5) 10.6-11.6 (11.2) 15.8-20.0 (17.1) 14.3-15.9 (15.4) 12.9-15.6 (14.1) 12.8-13.8 (13.3) 15.9-20.3 (17.8) 16.3-17.5 (16.9) 6.7-7.2 (7.0) 6.6-7.6 (7.0) 3.7-4.9 (4.4) 4.1-4.6 (4.3) 5.6-7.3 (6.2) 5.4-6.3 (5.9) 7.0-7.5 (7.3) 7.0-7.3 (7.2) 12.8-14.7 (13.9) 12.3-13.0 (12.7) 17.3-20.4 (18.9) 16.5-17.6 (17.0) 7.8-10.1 (8.8) 7.8-8.3 (8.1) 3.0-3.8 (3.4) 3.1-3.5 (3.3) 8.5-10.4 (9.6) 8.5-9.3 (8.9) 11.8-13.1 (12.4) 11.3-12.2 (11.6) on the belly. Four broad dark bands on flank, without or very faintly marked in specimens smaller than approxi- mately 160 mm SL. One small black spot in postorbital and suborbital areas, greater than pupil; seven to seventeen black spots on opercle, smaller than pupil. Black spots on body few or numerous (more than 70 in most specimens larger than about 160 mm SL), smaller than pupil, ocellat- ed, scattered on the back (missing in the predorsal area), a middle portion of flank, sometimes over upper and lower halves of the flank. No black spot on top of the head. Red spots few (less than about 20), ocellated, organized in two or three irregular longitudinal rows on median part of the body, and half of lower part of the flank. Commonly no red spots on flank in specimens larger than 160 mm SL, if red spots present in specimens larger than about 160 mm SL, they are almost covered with black dots; dorsal fin grey, with three or five rows of black spots (smaller than pupil), and one or two rows of red spots (smaller than pupil) in specimens smaller than about 120 mm SL. Caudal fin grey or dark grey; pectoral, anal and pelvic fins grayish. Adi- pose-fin plain greyish. Nine or ten mostly vertically elon- gated parr marks on the body, distinct in specimens up to at least about 160 mm SL. In life: General body colour silvery. Back and halves of upper part of flank silver, belly and halves of lower part of flank yellowish. All fins yellowish. A conspicuously black spots behind eye, smaller than pupil. Red spots few and with almost covered black pigment in specimens larger than about 160 mm SL, scattered on median part of the body, and half of lower part of the flank. Adipose-fin plain greyish, with very inconspicuous reddish margin. Nine or ten vertically elongated dark grey parr marks along middle part of flank. Distribution. Salmo ekmekciae inhabits clear and moderately swift-flowing water, with a substrate of stones and pebbles. It is only known from the Koprticay River and its tributaries in Antalya-Isparta province (Fig. 5). Sexual dimorphism. The snout of the male is more pointed than that of the female. The length of the head, and the length of the maxilla of the male are slightly greater than those of the female counterparts. Etymology. The species is named for Dr. Fitnat Guler Ekmekci (Turkiye), ichthyologist, in appreciation of her contribution to literature. Conservation status. Salmo ekmekciae is only known from Kopricgay River, most probably endemic to that area. The species is threatened by overfishing similar to other Salmonids and there are rainbow trout (Oncorhyn- chus mykiss) farms in the region. zse.pensoft.net 400 Kucuk, F. et al.: A new species of trout from the Mediterranean Sea drainage, Turkiye @ S.platycephalus © S.rizeensis @ S.tigridis @ S.ardahanensis |} ® S.brunoi @ S.abanticus © S.ekmekciae @ S.araxensis @ S.baliki @ Schilo ®@ S.coruhensis © S§.duhani @ S.euphrataeus © §fahrettini @ S.kottelati @ S.munzuricus @ S.murathani © S.okumusi @ S.opimus @ Slabecula Figure 5. Type localties of Salmo species in the Turkiye. Key to native Salmo species distributed in Tiirkiye 10 11 RECHSPOTST AKG SieL Val ey STOU OM dpe cnn ae cepted sed as contan ane vince samme as bean eecene ee 938 et oh rans an dinan erases See fas see ee kee S. baliki RecEspotstsiinallerstihicingeyeiOCiolli. om ee Macck ok. Lee sacpraauslh. lta eter lec te eae iina tina neltc acts inn dt