Apeer-reviewed open-access journal BioRisk 20: 37—57 (2023) 1 1 doi: 10.3897/biorisk.20.97598 RESEARCH ARTICLE & B lO R IS k https://biorisk.pensoft.net Genotype differences towards lead chloride harmful action Teodora Ivanova Todorova', Petya Nikolaeva Parvanova', Krassimir Plamenov Boyadzhiev', Martin Dimitrov Dimitrov’, Stephka Georgieva Chankova' | Lnstitute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Str, 1113, Sofia, Bulgaria Corresponding author: Teodora Ivanova Todorova (tedi_todorova@yahoo.com) Academic editor: G. Radeva | Received 16 November 2022 | Accepted 12 February 2023 | Published 15 May 2023 Citation: Todorova TI, Parvanova PN, Boyadzhiev KP, Dimitrov MD, Chankova SG (2023) Genotype differences towards lead chloride harmful action. In: Chankova S, Danova K, Beltcheva M, Radeva G, Petrova V, Vassilev K (Eds) Actual problems of Ecology. BioRisk 20: 37-57. https://doi.org/10.3897/biorisk.20.97598 Abstract The aim of the study was to throw more light on the PbCI, mode of action (MoA) depending on the genotype by the application of three model organisms and microbiological, biochemical, and molecular approaches. Three model systems — Chlamydomonas reinhardtii strain 137C — wild type (WT), Saccharomyces cerevisiae strain D7ts1, and Pisum sativum L. cultivar Ran1 and two experimental schemes — short- and long-term treatments were used. C. reinhardtii and S. cerevisiae cell suspensions (1x10° cells/ml) at the end of the exponential and the beginning of a stationary phase of growth were treated with various PbCI, concentrations (0.45—3.6 mM) for 2 hours. Lower PbCI, concentrations (0.03—0.22 mM) were also tested on C. reinhardtii 137C. Short-term treatment for up to 2 days with PbCl, concentrations in the range of 0.45—3.6 mM and long-term treatment for up to 10 days with concentrations in the range of 0.45—2.7 mM was performed on P sativum L. seeds and plants, respectively. Long-term treatment with a PbCI, concentration of 3.6 mM was not tested because of the very strong toxic effect (plant death). The following endpoints were used — for C. reinhardtii: cell survival, “visible” mutations, DNA double-strand breaks (DSBs), malondialdehyde (MDA), intracellular peroxides (H,O,), and photosynthetic pigments; for S. cerevisiae — cell survival, gene conversion, reverse mutation, mitotic crossing-over, DSBs, superoxide anions, MDA and glutathione (GSH); P sativum L. — germination and root length (short-term treat- ment), pro-oxidative markers - MDA, H,O, and photosynthetic pigments (long-term treatment). Copyright Teodora Ivanova Todorova et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 38 Teodora Ivanova Todorova et al. / BioRisk 20: 37-57 (2023) Genotype differences between C. reinhardtii (0.047 mM) and S. cerevisiae (1.66 mM) were observed by two endpoints: concentrations inducing 50% lethality (LD,,) and DSB induction. By contrast, no mutagenic effect was found for both unicellular test models. A slight toxic capacity of PbCl,, measured as inhibition of Pisum sativum L. seed germination and around 20% root length reduction was revealed after the treatment with concentrations equal to or higher than 1.8 mM. The variety of stress responses between the two plant test models was demonstrated by comparing MDA and H,O.,. A dose-dependent increase in HO, levels and a minor increase of MDA levels (around 9—15%) were measured when C. reinhardtii cells were treated with concentrations in the range of LD, — LD,, (0.03-0.11 mM). Analyzing the kinetics of MDA and H,O, in pea leaves, the most pronounced effect of concentration was shown for 2.7 mM. A decrease in the photosynthetic pigments was detected in the two experimental designs — short-term on C. reinhardtii and long-term on P sativum treatments. The pro-oxidative potential was also proven in S. cerevisiae based on increased levels of MDA and superoxide anions and decreased GSH. New information is gained that PbCl, can affect the DNA molecule and photosynthetic pigments via induction of oxidative stress. Our study revealed that the magnitude of stress response towards PbCl, is genotype-specific. Our finding that Chlamydomonas reinhardtii is a sensitive test system towards PbCl, contributes to good strategies for revealing very low levels of contaminants present chronically in main environmental matrices. This is the first report, as far as we know, affirming that PbCI, can induce DSBs in Chlamydomonas reinhardtii and Saccharomyces cerevisiae. Keywords Chlamydomonas reinhardtii, DNA damaging potential, lead chloride, mutagenicity, Pisum sativum L., pro- oxidative effect, Saccharomyces cerevisiae, toxicity/ genotoxicity Introduction Currently, environmental pollution with various chemicals is considered as an impor- tant environmental problem provoked to a large extent by human activity (Zulfiqar et al. 2019). Due to this fact, strict legislation was created, controlling both manufac- turing processes, the levels released into nature as well as maximum levels of certain contaminants in food, water, air, etc. (See Commission Regulation EC documents 2016/582, 2022). Over the years significant contamination of the main ecological matrices with heavy metals such as cadmium, lead, chromium, copper, zinc, mercury, and arsenic has been reported by different authors (Schulin et al. 2007; Kabir et al. 2012; Satta et al. 2012). The increased levels of heavy metals as a result of human activity — industry, agriculture (irrigation with contaminated water, use of mineral fertilizers), waste burn- ing, burning of fuel, road transport, etc. has caused concern about both the health of nature and man (Jarup 2003; European Commission 2013; Toth et al. 2016). Heavy metals commonly present in the form of cations possess two main features: quick accumulation and slow release. Mercury, cadmium, and lead are considered sub- stantial risk factors for the biota. Genotype differences towards lead chloride harmful action 39 Lead (Pb) is considered the second most toxic metal after arsenic (As) as it is very toxic for all living organisms (ATSDR 2019; Zulfigar et al. 2019). Sources of lead could be dust, old paint, different user products, leaded gasoline, batteries, smelting and refining processes, etc. (Can et al. 2008; Wani et al. 2015; Kumar et al. 2020). The poisoning capacity of lead has been known since ancient times. In the last century, toxic effects of lead and its compounds were the focus of scientists. A lot of data were collected concerning lead’s high accumulation in different body tissues and organs as well as its toxic capacity as a result of exposure to contaminated water, air, and food (Balali-Mood et al. 2021; Mohanta et al. 2022). Currently, new experimental data were gathered suggesting lead’s indirect mecha- nisms of genotoxicity — the production of free radicals, altered expression of DNA repair genes, and inhibition of DNA repair systems (Garcia-Leston et al. 2010; Hemmaphan and Bordeerat 2022). For the first time, evidence was published by Liu et al. (2018) concerning the DNA damaging potential of lead by promoting oxidative stress as well as the methyla- tion of DNA repair genes in human lymphoblastoid TKG6 cells. Everything written above demonstrated that lead and its compounds have been characterized as toxic/genotoxic in a variety of test systems but little is currently known about their mutagenic, clastogenic, carcinogenic capacity, and mode of action (MoA). In short, at present, information concerning the potential pro-oxidative, mutagenic, and DNA damaging potential of lead and its compounds as well as its MoA, is scarce. Here we aimed to try to compensate for this gap to some extent using three model organisms and different approaches — microbiological, biochemical, and molecular in order to shed more light on the MoA of PbCl, depending on the genotype. This investigation was performed using three model organisms — unicellular green alga Chlamydomonas reinhardtii, Saccharomyces cerevisiae, and Pisum sativum L. Unicellular green algae, including C. reinhardtii, are a robust model for plant cells in genetic, molecular, physiological, and eco-toxicological studies due to their advan- tages which have been well described previously (Chankova et al. 2000, 2005, 2007, 2014; Chankova and Bryant 2002; Dimitrova et al. 2007). On the other hand, results obtained in this model organism could be extrapolated to a variety of plant organisms (Merchant et al. 2007; Li et al. 2020). S. cerevisiae is an extensively used model for studying the response of heavy metals due to the highly conservative mechanisms related to different stress response pathways as well as protein similarity with higher eukaryotes including humans (Rajakumar et al. 2020; Todorova et al. 2015a). The entirely sequenced S. cerevisiae genome reveals around 31% similarity to the human genome and thus results could be extrapolated at human level (Todorova et al. 2015b). Pisum sativum 1. (garden pea) is a classic model organism used in biochemical, physiological, and genetic studies of plants. In addition, it should be mentioned that Pisum sativum L. is one of the most important food items among legume crops (Galal et al. 2021). The variety Ran 1, is a widely popular agricultural crop in Bulgaria. 40 Teodora Ivanova Todorova et al. / BioRisk 20: 37-57 (2023) Materials and methods Model systems, cultivation, and experimental schemes Three model systems — Chlamydomonas reinhardtii strain 137C — wild type (WT), Saccharomyces cerevisiae strain D7ts1, and Pisum sativum L. cultivar Ran1, and two ex- perimental schemes — short — and long-term treatments were used. PbCI, of analytical grade was purchased from Valerus LTD. Short-term treatment Chlamydomonas reinhardtii 137C was cultivated at standard conditions — light of 70 umol/m’.s and t = 25 + 3 °C and Saccharomyces cerevisiae strain D7ts1 was cul- tivated at t = 30 °C, 200 rpm to the end of the exponential and the beginning of a stationary phase of growth. After that, cell suspensions with a density of 1x10° cells/ ml were treated with various PbCl, concentrations (0.45, 0.9, 1.8, 2.7, and 3.6 mM) for 2 hours. Additionally, due to the very low cell survival of C. reinhardtii 137C after the treatment with this concentrations’ range, lower concentrations were also tested — 0.03, 0.06, 0.11, and 0.22 mM. Pisum sativum L. seeds were treated with PbCl, at concentrations of 0.45, 0.9, 1.8, 2.7, and 3.6 mM for 24 and 48 hours to evaluate both the germination and the root length. Long-term treatment Pea plants were grown on a Knop medium until they reached the third physiologically developed leaf (around 10 days) under controlled conditions in a growth chamber NUVE GC 400, light regime 16/8 day/night; a temperature of 24 + 2 °C; humid- ity of 70 + 5%. After that, the Knop medium of the plants was replaced with PbCl, solutions with various concentrations in the range of 0.45, 0.9, 1.8, and 2.7 mM for 10 days. Experiments for pro-oxidative potential were conducted in order to study the kinetics of these markers. Plants were grown for 2, 5, 7, and 10 days in a medium contaminated with different PbCl, concentrations written above; leaves’ samples were subsequently collected and biochemical analyses were performed. The genotoxic potential of PbCl, on Chlamydomonas reinhardtii was evaluated as de- scribed in Dimitrova et al. 2014. In short, a “clonal assay” was performed to evaluate the colony-forming ability of the strain after the treatment, counting macro-colonies’ survival. On Saccharomyces cerevisiae, the Zimmermann test was used (Zimmermann et al. 1984). The survival fraction (SF) (Bryant 1968) as well as concentrations that can induce 20, 50, and 80% lethality was calculated (Lidanski 1988; Dimitrova et al. 2014). The toxicity was evaluated based on the inhibition of Pisum sativum L. germination and reduction of root length. Genotype differences towards lead chloride harmful action 4] Oxidative stress markers Intracellular malondialdehyde (MDA) was measured at 532 nm and 600 nm as de- scribed by Dhindsa et al. (1981). H,O, content was measured at 390 nm (Heath and Packer 1968). Pigment contents were measured at 663, 645, and 452.5 nm (Arnon 1949; Dimitrova et al. 2007) on Ultrospec 2100 pro spectrophotometer (Amersham Biosciences). Total glutathione was measured according to Zhang (2000). Superoxide anions were evaluated as described in Stamenova et al. (2008). Mutagenic action A test of “visible mutant colonies” was applied to evaluate the mutagenic potential of PbCL, on the unicellular algae after that the Mutagenic Index was calculated as de- scribed by (Dimitrova et al. 2007). Changes in size, morphology, and pigmentation of surviving colonies were analyzed (Shevchenko 1979). Zimmermann’s test (Zimmermann et al. 1984) with Saccharomyces cerevisiae dip- loid strain D7ts1 (MATa/a ade2-119/ade2-40 trp5-27/trp5-12 ilv1-92/ilv1-92 ts1/ts1) was applied as described before (Todorova et al. 2015b). The test provides simultane- ous detection of cell survival, mitotic gene conversion at the trp-5 locus, and reversion mutations in the z/vJ locus. DNA damaging potential DNA double-strand breaks (DSBs) were measured by Constant field gel electrophore- sis (CFGE) as described in Chankova and Bryant (2002), Chankova et al. (2005), and Todorova et al. (2015a, 2019). Statistical analysis Data were analyzed using GraphPad Prism5 software (San Diego, USA) and the sta- tistical analysis was done by one-way and two-way analysis of variances (ANOVA) fol- lowed by the Bonferroni posthoc multiple comparisons test. Linear correlation, using Pearson Product- Moment Correlation Coefficient analysis (PMCC, or r) and coef- ficient of determination (R’) were determined. All the experiments were performed in triplicate. Data are presented as mean + SEM (standard error of the mean). Results Genotoxicity in Chlamydomonas reinhardtii and Saccharomyces cerevisiae Genotype-related differences in the response toward lead treatment were identi- fied. Treatment with concentrations in the range of 0.45-3.6 mM PbCI, resulted 42 Teodora Ivanova Todorova et al. / BioRisk 20: 37-57 (2023) A) 425 B) 1.25 @ C. reinhardtii TH S. cerevisiae < < fe] 1.00 A>) 1.00 “ de Oo 15) & 0.75 © 5.0.75 ws LL - a2 gf > 0.50 2 0.50 5 2 | = HM 0.25 w 0.25 0.00 0.00 0.0 05 1.0 15 20 25 3.0 3.5 4.0 0.00 0.05 0.10 0.15 0.20 0.25 Lead chloride concentration (mM) Lead chloride concentration (mM) C) Chlamydomonas Saccharomyces reinhardtii cerevisiae (WT) (D7ts1) Lead chloride concentration (mM) LD20 0.03 0.47 LDSO 0.05 1.66 LD80 0.14 3.67 Figure |. A Cell survival of Chlamydomonas reinhardtii (black circle) and Saccharomyces cerevisiae (black square) after the treatment with PbCL, concentrations in the range of 0.45—3.6 mM for 2 hours B cell sur- vival of Chlamydomonas reinhardtii after the treatment with PbCL, concentrations in the range of 0.03—0.22 mM for 2 hours C three doses of lethality were calculated. Results are from at least three experiments with independently grown cell cultures and presented as mean + SEM. Asterisks represent statistical significance (ns P > 0.05; *** P< 0.001). Where no error bars are evident, they are equal to or smaller than the values. in a differential response of Chlamydomonas reinhardtii and Saccharomyces cerevisiae (Fig. 1A). Dose-dependent decrease of cell survival (P<0.0001) up to 25% was ob- tained for S. cerevisiae D7ts1 (Fig. 1A). All the concentrations tested resulted in around 10% cell survival of C. reinhardtii 137C. No effect of the concentration was calculated. Further, lower PbCl, concentrations were tested in C. reinhardtii 137C (Fig. 1B). A dose-dependent decrease of survived colonies was obtained when concentrations in the range of 0.03—0.22 mM (P < 0.0001) were applied (P < 0.0001). Based on the survival data, three doses of lethality were calculated (Fig. 1C). Data revealed that an around 30-fold lower dose can cause 50% lethality in C. reinhardtii in comparison with S. cerevisiae. Toxicity in Pisum sativum L. A slight toxic capacity of lead chloride on P sativum L. was evaluated. Around 10% inhibi- tion of seed germination (Fig. 2A) and around 20% root length reduction (Fig. 2B) were calculated after the treatment with PbCI, concentrations equal to or higher than 1.8 mM. Genotype differences towards lead chloride harmful action 43 B) 120 100 80 60 40 20 Root length (%) Germination (%) ar oe : ') 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 Lead chloride concentration (mM) Lead chloride concentration (mM) Figure 2. Toxic potential of various concentrations of PbCl, on Pisum sativum L. presented as percent germination (A) and root length (B). Results are from at least three experiments and presented as mean + SEM. Asterisks represent statistical significance (ns P > 0.05; *** P < 0.001). Where no error bars are evident, they are equal to, or smaller than, the values. Even though the decreases in both parameters were calculated as statistically sig- nificant to the corresponding control sample, they can hardly be regarded as significant from a biological point of view. Pro-oxidative potential after short-term treatment with PbCI, on the unicel- lular model systems — C. reinhardtii and S. cerevisiae The pro-oxidative potential of PbCl, was studied by several endpoints in the range of concentrations corresponding to LD,,, LD,, and LD,,. A very minor increase in MDA levels (around 9-15%) (Fig. 3) was measured when C. reinhardtii cells were treated with concentrations in the range of LD, —LD,, (0.03—0.11 mM) (P< 0.001). Concerning the other pro-oxidative marker, dose-dependent statistically signif- cant higher levels of intracellular peroxides were obtained (Fig. 3). More than 3-fold higher levels of both MDA and H,O, were induced after the treatment with concentrations that can cause over 80% cell lethality. As a next step, the levels of photosynthetic pigments were evaluated (Fig. 3). A similar — around 50-60% decrease in chlorophyll a (chl a), chlorophyll 4, and carot- enoids were calculated without concentration dependence (P < 0.0001). Concerning the other model system — S. cerevisiae, treatment with concentrations corresponding to 1B EDS, and Ls. resulted in a statistically significant dose-de- pendent increase in the superoxide anions’ levels (Table 1). The most pronounced effect — more than 2-fold higher levels of superoxide anions was calculated at LD,, (P < 0.01). Around a 2-fold increase with no effect of the concentration was obtained for MDA (Table 1). At the same time, a decrease in the total glutathione levels was meas- ured for the same experimental conditions (P < 0.0001). 44 Teodora Ivanova Todorova et al. / BioRisk 20: 37-57 (2023) 4007-6 mpa -H,0, “‘“ chla “¥ chi 6b’ -@® carotenoids eee Oxidative stress markers (% of the control) 0.00 0.05 0.10 0.15 0.20 £0.25 Lead chloride concentration (mM) Figure 3. Oxidative stress induced by PbCL, at concentrations range of 0.03—0.22 mM in Chlamydomonas reinhardtii. Data are presented as mean + SEM. All the results are from at least three independent experi- ments. Statistical significance was calculated among all the samples and to the controls (P < 0.001). Where no error bars are evident, they are equal to or smaller than the values. Table |. Oxidative stress markers in Saccharomyces cerevisiae after the treatment with various PbCL, con- centrations for 2 hours. Superoxide anions (pM O2-/cell) MDA (mM/g sample) GSH (mmol GSH/g sample) Control 0.404 + 0.01 0.128 + 0.039 0.003 + 0.00006 LD,, 0.360 + 0.04ns 0.260 = 0.027* 0.002 +0.00006*** LD,, 0.626 + 0.03* 0.280 = 0.012* 0.001 +0.00006*** LD 0.965 + 0.05*** 0.296 + 0.009** 0.001 £0.00007*** Mean values were calculated from at least 3- experiments by independently grown cell cultures. Asterisks represent statistical significance between the control and the samples (ns P > 0.05; * P < 0.05; ** P< 0.01; *** P< 0.001). Pro-oxidative potential after long-term treatment with PbCI, on P sativum L. No statistically significant differences were measured in the levels of MDA, intracellu- lar peroxides, and the photosynthetic pigments (chl a, chl 6, chl a/6, and carotenoids) in control samples grown without PbCl, for 2,5, 7 and 10 days (data not shown). The calculation of kinetics data shows that plants grown in an environment con- taminated with different PbCl, concentrations for 2 days did not suffer at these ex- perimental conditions. No statistically significant increase in MDA and intracellular peroxides was defined (data not shown). Genotype differences towards lead chloride harmful action Table 2. Kinetics of the oxidative stress markers MDA and intracellular H,O, in Pisum sativum L. after long-term treatment (2, 5, 7, and 10 days) with various PbCl, concentrations. ! Concentrations Marker Days 5 7 10 Control MDA 100 100 100 EO), 100 100 100 0.45 mM MDA 140+10.87*** IS342.867"" 12542.66** FE), 99+3.47 ns 96+9.80 ns 11S. SSens 0.9 mM MDA 13146.34** IDS 163" 11143.70 ns HO, 932275308 91+2.46 ns 10043.32 ns 1.8 mM MDA 121+7.64* 123£3.86* 127+4.77** Fs Oe 97+3.49 ns 104+10.02ns 12848.65** 2.7 mM MDA 129£9.88** 14345.54"* LoGP337"— FREY 11943.12 ns 12929:85 7°" 172+9.43°* ' Results are calculated as a percent of the control. Data are presented as mean + SEM. All the results are from at least three independent experiments. Asterisks represent statistical significance between the control and the treated samples (ns P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001). ‘Treatment with PbCI, concentrations from 0.45 to 1.8 mM resulted in an approxi- mately similar increase in both MDA and HO, levels (Table 2). No effect of exposure time was found. The most pronounced oxidative stress measured as increased MDA and HO, contents was calculated when plants were grown for 10 days in a contami- nated with 2.7 mM PbCl, medium. A similar relationship was defined concerning the other marker for oxidative stress induced by PbCl, and measured as levels of H,O,. The only statistically significant increase was calculated when plants were treated for 10 days with concentrations of 1.8 and 2.7 mM (Table 2). Based on these results, it could be speculated that in our experimental conditions, PbCL, most probably induces lipid peroxidation in Pisum sativum L. plants. Here, we have not discussed the effects obtained after the applications of the most favorable for Pisum sativum L. plants experimental conditions — the lowest concentra- tion of 0.45 mM PbCL, and two days duration of plants growing at different PbCl, concentrations due to the fact that both factors have not affected in any way photosyn- thetic pigments contents (data not shown). Concerning the other concentrations in the range of 0.9-2.7 mM, data revealed that the photosynthetic pigments are affected mostly by the concentration and not by the treatment time (Fig. 4A—C). The only time-dependent decrease (Fig. 4B) was detected for chl 6 and treatment with 0.9 mM (P < 0.001). Our results have revealed the harmful potential of PbCl, on P sativum L. plants grown for 2, 5, 7, and 10 days in a medium contaminated with various PbCl, con- centrations. We found a statistically significant decrease in the levels of photosynthetic pigments we analyzed by around 40—G0%, as well as no changes in the chl a/b ratio compared with those in the controls samples. 46 Teodora Ivanova Todorova et al. / BioRisk 20: 37-57 (2023) A ms 100m, @ 0.9mM 4H 1.8mM 4 2.7mM B) F00 ‘* 0.9mM 4 1.8mM -4 2.7mM — 80 = > 60 >, : = © 40 6 = pe 2 20 2 r= = 5 oO 0 0 0.0 2.5 5.0 7.5 10.0 12.5 0.0 2.5 5.0 7.5 10.0 12.5 Treatment time (days) Treatment time (days) C) 100 ® 0.9mM & 1.8mM -& 2.7mM Carotenoids (%) 0.0 2.5 5.0 7.5 10.0 12.5 Treatment time (days) Figure 4. Kinetics of the photosynthetic pigments chlorophyll a (A), chlorophyll 6 (B), and carotenoids (C) in Pisum sativum L. treated with various PbCl, concentrations for different time (2, 5, 7, and 10 days). Results are calculated as a percent of the control. Data are presented as mean + SEM from at least three independent experiments. Asterisks represent statistical significance between the control and the treated samples (*** P < 0.001). Where no error bars are evident, they are equal to, or smaller than. the values. Our further steps were to clarify whether PbCl, would have some mutagenic and DNA damaging capacity on both unicellular test systems — Chlamydomonas reinhardtii strain 137C and Saccharomyces cerevisiae strain D7ts1 at our experimental conditions. Mutagenic activity No mutagenic potential of PbCL, was revealed on both unicellular organisms, despite the well-pronounced genotoxic effect. The mutagenic index in Chlamydomonas reinhardtii was calculated to be less than 2.5, indicating no mutagenic capacity of PbCl, in the tested concentration range. The mitotic gene conversion and reverse mutations in Sac- charomyces cerevisiae were comparable with the control untreated cells (data not shown). DNA damaging activity To throw more light on the mode of action (MoA) of PbCL, its potential to induce double-strand breaks in DNA was evaluated on C. reinhardtii (Fig. 5A, C) and Genotype differences towards lead chloride harmful action 47 a 0. 0.0 0.1 0.2 0.3 0.4 0.5 0 A 2 3 4 Lead chloride concentration (mM) Lead chloride concentration (mM) L234 5 6 FJ 8M ATL 2 12 & 458 6 T S$ C) “PTT re tert: Dp) @eee ea0eq ie ee ee Ue Figure 5. Levels of primary induced double-strand breaks in DNA of Chlamydomonas reinhardtii (A, C where 1,2 — control; 3, 4 — 0.03 mM; 5, 6 — 0.06 mM; 7, 8 — 0.11 mM; 9, 10 — 0.22 mM; 11, 12 — 0.45 mM) and Saccharomyces cerevisiae (B, D where 1, 2 — control; 3, 4 — 0.5 mM; 5, 6 — 1.7 mM; 7, 8 — 3.7 mM) after the treatment with various PbCl, concentrations. Values represent the mean frac- tion of DNA released (FDR). Data are presented as mean + SEM. All the results are from at least three independent experiments (ns P > 0.05; ** P< 0.01; *** P< 0.001). Where no error bars are evident, they are equal to, or smaller than, the values. S. cerevisiae (Fig. 5B, D). Our data is the first experimental evidence for the induction of DSBs after the treatment with PbCl, Again, genotype-related differences in the response to PbCl, were established. A statistically significant dose-dependent DSBs increase was found after the application of PbCl, in concentrations up to LD,, — 0.11 mM for C. reinhardtii (Fig. 5A, C). No statistically significant difference was calculated between the effect of the treatment with 0.11 and 0.22 mM PbCI, — around 2-fold elevated DSBs levels. The treatment with a PbCI, concentration of 0.45 mM resulted in significant DNA degradation (Fig. 5C). Interesting results were obtained when comparing the DSB induced by concentra- tions corresponding to the calculated LD. Concentrations corresponding to LD,, in both test systems resulted in a similar induction of DSBs — around 2-fold (Fig. 5A, B). Around 1.5-fold higher DSB levels in C. reinhardtii and more than 2-fold in S. cerevi- siae were measured after the treatment with the respective LD,, concentrations. LD,, resulted in a small but statistically significant (P<0.001) increase in DSB levels for C. reinhardtii (Fig. 5A) and no effect on S. cerevisiae (Fig. 5B). Comparing the concentration ranges, it should be pointed out that concentrations used in Saccharomyces cerevisiae experiments were approximately 10-fold higher than those used for Chlamydomonas reinhardtii. 48 Teodora Ivanova Todorova et al. / BioRisk 20: 37-57 (2023) In order to reveal whether a relationship exists among the pro-oxidative, DNA damaging, toxic/genotoxic, and mutagenic potential of lead chloride, correlation anal- ysis was performed for both unicellular model systems. Correlation analysis In Chlamydomonas reinhardtii (Table 3), the decrease in cell survival (SF) was found to correspond strongly with an increase in the H,O, (P< 0.001) and DSB (P< 0.001). A good correlation was calculated between higher levels of H,O, and low levels of all the photosynthetic pigments (Table 3). Based on this and the graphically presented changes in the markers studied (Fig. 6A), it could be speculated that the Pb-induced oxidative stress in terms of in- tracellular peroxides probably may participate in the induction of DSBs, resulting in a decrease in the cell survival. Concerning the other unicellular organism — Saccharomyces cerevisiae, a statistically significant strong correlation was calculated between the decrease in cell survival and the induction of superoxide anions (R = -963, P < 0.05) (Fig. 6B). The increase in DSBs levels (Fig. 6C) was found to strongly correlate to the increase in MDA levels (R = 0.947, P < 0.05) and the decrease in GSH (R = -0.964, P< 0.05). The changes in the markers could be observed in Fig. 6B, C. A) B) yea @ Cell survival -@® Superoxide anions — _ ov ° ct) 0 oe D = fh ff £ Oo oO 0.00 0.05 £0.40 ° # 0.45 (mM) (mM) C) Change of markers “9 1 2 3 4 Lead chloride concentration (mM) Figure 6. Marker’s correlation in C. reinhardtii (A) and S. cerevisiae (B) after 2 hours treatment with LD,, and LD,,. PbCI2 in concentrations corresponding to LD,,, Genotype differences towards lead chloride harmful action 49 Table 3. Correlation analysis among the studied endpoints in a model system Chlamydomonas reinhardtii after the treatment with PbCl.. SF DSB MDA H,O, chl a chl b Car SF -0.942** -0.717 -0.943** 0.763 0.944** 0.721 DSB 0.587 0.941** -0.916* “979"* -0.863 MDA 0.825 -0.561 -0.653 -0.646 H,O, -0.888* -0.953** ——_-0.889* chl a 0.907* 0.985** chl b 0.871 Car ' Studied endpoints in the range of PbCL, concentrations 0.03—0.22 mM — MDA, H.O,, chl a, chl 4, carotenoids (car), survival fraction (SF), and double-strand breaks (DSB). Values represent the R* for linear correlation. A correlation coefficient (R) higher than 0.900 denotes a strong positive correlation and higher than -0.900 — a strong negative cor- relation (*P < 0.05; ** P< 0.01; *** P< 0.001). Discussion Lead is a very toxic non-trace metal with well-proven both poisonous and genotoxic ca- pacities (Oztetik 2021; Riyazuddin et al. 2022; Chmielowska-Bak et al. 2022). Recent- ly, it was reported that the bioactivity of lead and its compounds can be attributed to its pro-oxidative capacity (Devéz et al. 2021; Chmielowska-Bak et al. 2022). As stated in the introduction, information concerning the potential pro-oxidative, mutagenic, and DNA-damaging potential of lead and its compounds as well as its MoA is scarce. Our main aim was to supply new information about the MoA of PbCl.,. This aim has provoked us to focus our attention on two main items: the first relates to the evalu- ation of mutagenic and DNA-damaging capacity of PbCl, on two model test systems; the second was to analyze the possible contribution of oxidative stress in these biologi- cal events using various endpoints. According to the WHO, a battery of test systems and endpoints evaluating differ- ent adverse effects at different levels is a good strategy for obtaining reliable informa- tion about MoA of different xenobiotics. Additionally, the application of several test systems provides more reliable information as some tested materials, such as chelating agents, heavy metals, and some surfactants with unusual physical and chemical prop- erties, may cause practical and test system-specific difficulties, and compromise the outcome of the test by providing false-negative or -positive results (Turkez et al. 2017). The advantages of the test systems were described briefly in the introduction. ‘They were chosen based on the fact that each of them may provide information concerning different endpoints. Such a strategy may provide more detailed information concern- ing the MoA of xenobiotics. For the purpose of our study, this investigation has gone through several consecutive steps, using a complex of approaches — microbiological, biochemical, and molecular. The first one was to evaluate the toxic/genotoxic capacity of tests-systems and the results obtained were compared with some experimental data of other authors. The toxic capacity of PbCl, on P sativum L. cultivar Ran] was evaluated by two endpoints — inhibition of seed germination and reduction of root length. It can be said 50 Teodora Ivanova Todorova et al. / BioRisk 20: 37-57 (2023) that the minor toxic effect of PbCI, in our experimental scheme is not concentration- dependent, in spite of the fact that differences were statistically significant. Our obser- vation confirms the one reported by Silva et al. (2017) that growth-related parameters could not be considered as the most sensitive and reliable endpoints to evaluate Pb toxicity. The slight toxicity of PbCl, on Pisum sativum L. presented as slightly inhibited germination and reduced root length have been reported by other authors and other plants such as Parkinsonia aculeata and Pennisetum americanum (Shaukat et al. 1999); Ipomoea aquatica Forsk (Ni’am and Yuniati 2021) and Hordeum vulgare L.) (Vasi¢ et al. 2020). Ni'am and Yuniati (2021) speculate that the stronger effect on root length than germination could be due to the low permeability of seed testa to lead. Based on the toxic/genotoxic results, additional data were provided concerning the sensitivity of C. reinhardtii strain 137C to PbCI, compared with S. cerevisiae. The high sensitivity of C. reinhardtii was previously reported for chlorpyrifos (Todorova et al. 2020). This finding is important because very sensitive genotypes are a very good tool for revealing very low levels of contaminants present chronically in main environmen- tal matrices, and can result in long-term disturbance of biota. Further, the pro-oxidative potential of PbCl, was confirmed in all the model sys- tems used by us. The approach applied by us on the test systems covers a wide range of reactive oxygen species (ROS). It is well-known that the first ROS produced is the superoxide anion (Sullivan and Chandel 2014). A dose-dependent increase was calcu- lated for the levels of superoxide anions in S. cerevisiae D7ts1, which is in accordance with such findings in S. cerevisiae haploid strains (Dimitrov et al. 2011; Sousa and Soares 2014) and Pisum sativum L. root cells (Malecka et al. 2001). It is well-known that the O,~ is easily converted to H,O, by the enzyme mitochondrial superoxide dismutase (Sod2) (Herrero et al. 2008; Sousa and Soares 2014). Thus, we can assume that probably part of the superoxide anions could be converted to peroxides. As a next step, the levels of intracellular peroxides were studied. Data revealed that P sativum L. and C. reinhardtii responded differently to PbCl,-induced oxidative stress in terms of intracellular peroxides. While no effect was observed for most of the concentrations tested on P sativum L., a dose-dependent increase was obtained for C. reinhardtii. It can be suggested that the genotype also plays a role in the response to the PbCL,- induced oxidative stress. The next marker for oxidative stress studied was MDA. Interestingly, the response significantly varies depending on the genotype. Our study provides evidence that al- though an increase in the MDA levels has been observed for all the model systems, the sensitivity of the marker depends on the genotype and the experimental conditions. In the unicellular organisms, MDA was not affected in a dose-dependent way, sug- gesting that it may not be the primary consequence of PbCI, treatment for 2 hours. Oppositely, in our long-term experiments on P sativum L., the levels of MDA were found to be the most increased compared to the rest of the pro-oxidative stress mark- ers. Contradictory data exist concerning the induction of lipid peroxidation by lead and its compounds. According to some authors, lead may play an indirect role in lipid peroxidation (Sivaprasad et al. 2004; Hasanein et al. 2017). Based on results in the Genotype differences towards lead chloride harmful action 1 present work, it may be speculated that PbCL,-induced lipid peroxidation depends on the experimental conditions. The last marker studied was GSH. Its role in the antioxidant defense against vari- ous stressors is well documented (Perez et al. 2013). The results presented in this work also confirm those published by Perez et al. (2013) that PbCI, reduces the levels of GSH. The authors suggest that such a reduction could be explained by the high afhn- ity of the GSH thiol group to Pb and thus the formation of Pb-GSH complexes in the cytosol, decreasing the level of GSH (Perez et al. 2013). In addition to the well-known poisonous capacity of lead and lead compounds, our results confirmed and extended the current state of knowledge regarding their indi- rect mechanism of genotoxicity via induced oxidative stress. The specificity of induced radicals was found to depend on genotype and experimental conditions. Further, the effect of PbCl, on the photosynthetic machinery was evaluated. Inter- estingly, all the photosynthetic pigments in Chlamydomonas reinhardtii and Pisum sa- tivum L. decreased in a similar way by around 40-50% in both experimental schemes — short-term and long-term treatment without concentrations or time-dependence. Contradictory data exist concerning the effect of lead on photosynthetic pigments. Some studies point out that lead treatment may result in a decrease in the chlorophyll content of Phaseolus vulgaris and Lens culinaris which may be attributed to the ability of lead to replace the magnesium (Mg) in the chlorophyll ring (Irfan et al. 2010; Rai et al. 2016) as well as in other plants (Ruley et al. 2006; Yang et al. 2015). Other au- thors have reported that Pb does not affect the photosynthetic pigments in Scenedesmus acutus, Schroederia sp. (Dong et al. 2022), and Pisum sativum (Rodriguez et al. 2015). According to Zheng et al. (2020), the reduction of cell growth of Chlamydomonas reinhardtii could be due to the reduction of the chlorophyll content. The Pb-induced disturbance of photosynthesis in microalgae has been proposed as a major cause of cell death by Li et al. (2021). In our study, no statistically significant correlation was obtained between these markers. The SF was found to be negatively affected by HO, which confirms other findings that the metal-induced oxidative stress in Chla- mydomonas reinhardtii is related to growth inhibition (Bertrand and Poirier 2005; Stoiber et al. 2013). It should be taken into account that the increase in H,O, also correlates to the decrease in photosynthetic pigments. Here we can speculate that PbCI, can affect DNA molecules and photosynthetic pigments via the induction of oxidative stress. In the present work, an attempt was made to evaluate the potential mutagenic and DNA damaging potential of PbCl,. No mutagenic effect was obtained for both unicellular organisms — C. reinhardtii and S. cerevisiae at our experimental schemes. Our results confirm those published by Francisco et al. (2018) that Pb does not possess a mutagenic effect on Allium cepa root meristematic cells. In the present work, two tests for mutagenic activity were applied each of them providing information con- cerning various types of genetic alternations. Our data provide indirect evidence that PbCl, toxicity at the tested concentrations may not be related to the induction of point mutations, impaired cell division; micro-chromosomal aberrations; altered cell 52 Teodora Ivanova Todorova et al. / BioRisk 20: 37-57 (2023) wall structure and composition, mitotic gene conversion and mitotic crossing-over (Zimmermann et al. 1984; Dimitrova et al. 2007). To the best of our knowledge, the present work provides the first evidence that PbCl, induces DSBs in Chlamydomonas reinhardtii and Saccharomyces cerevisiae. It could be speculated that the Pb-induced oxidative stress could be the major mecha- nism for the induction of double-strand breaks in DNA which, in turn, may partially result in cell death. Conclusion Our study revealed that the magnitude of stress response towards PbCl, is genotype- specific. Chlamydomonas reinhardtii 137C is a more sensitive to PbCl, model than Saccharomyces cerevisiae D7ts1 and Pisum sativum L. cultivar Ran1. The approach ap- plied by us provided additional information concerning the mode of action of PbCl.. The toxic/genotoxic and DNA-damaging potential of PbCl, may be a result of the pro-oxidative effect. This is the first report, as far as we are aware, that lead chloride may induce DSB in Chlamydomonas reinhardtii and Saccharomyces cerevisiae. Our pro- posed approach — a battery of test systems and various endpoints could be considered a promising tool in the ecotoxicological assessment of various xenobiotics. Acknowledgements This work was supported by a grant from the National Science Fund, Ministry of Education and Science, Project No. KP-06-PN44/3. References Arnon DI (1949) Copper enzymes in isolated chloroplasts. 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