JHR 88: 85—102 (2021) ee, JOURNAL OF eet nnacnn nn doi: 10.3897/jhr.88.7594 | RESEARCH ARTICLE ) Hymenopter a 4 https://jhr.pensoft.net Te Inarasional Society of Hymenopeeriss, RESEARCH First record of the Sulia glaesaria Simutnik, 2015 (Hymenoptera, Chalcidoidea, Encyrtidae) from Rovno amber Serguei A. Simutnik', Evgeny E. Perkovsky', Mykola R. Khomych’, Dmitry V. Vasilenko?* | LL. Schmathausen Institute of Zoology, National Academy of Sciences of Ukraine, Kiev, 01601 Ukraine 2 Vil. Voronky, Varash Distr, Rovno Region, 34330 Ukraine 3 Borissiak Paleontological Institute, Russian Academy of Sciences, Moscow, 117647 Russia 4 Cherepovets State University, Cherepovets, Vologda Region, 162600 Russia Corresponding author: Serguei A. Simutnik (simutnik@gmail.com) Academic editor: Petr Jansta | Received 29 September 2021 | Accepted 13 October 2021 | Published 30 December 2021 Attp://zoobank. ore/4DC9I2DF2-AD5D-410B-A63D-7AC53D3C13C7 Citation: Simutnik SA, Perkovsky EE, Khomych MR, Vasilenko DV (2021) First record of the Sulia glaesaria Simutnik, 2015 (Hymenoptera, Chalcidoidea, Encyrtidae) from Rovno amber. Journal of Hymenoptera Research 88: 85-102. https://doi.org/10.3897/jhr.88.75941 Abstract Sulia glaesaria Simutnik, 2015 (Chalcidoidea, Encyrtidae), originally described from late Eocene Danish amber, is reported in coeval Rovno amber. A revised diagnosis of this genus is provided based on the new specimen and high-resolution photomicrographs of the holotype. Some character states, such as a short radicle, clypeus with long lateral margins, the presence of a strigil and basitarsal comb, spur vein of the hind wing, costal cell of hind wing along entire marginal vein with single line of long setae, and almost vertical syntergum with abruptly reflexed extension apically are reported in this species for the first time. Keywords Acropleural sulcus, acropleuron, Danish amber, Eocene, fossil Encyrtidae, syntergum Copyright Serguei A. Simutnik et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 86 Serguei A. Simutnik et al. / Journal of Hymenoptera Research 88: 85-102 (2021) Introduction Our research on several amber collections shows that the Encyrtidae is one of the most widespread families of Chalcidoidea in Eocene ambers (Simutnik 2001, 2002, 2007, 2015a, 2020; Simutnik and Perkovsky 2017, 2018a, b, c, 2020; Simutnik et al. 2014, 2021) along with Mymaridae, Aphelinidae and Trichogrammatidae. Most extant fami- lies of Chalcidoidea, including Tanaostigmatidae s.s., are, however, still unknown from the late Eocene. The large and distinctive chalcidoid wasp Leptoomus janzeni Gibson, 2008 from Baltic amber was previously attributed to Tanaostigmatidae s.l. along with the extant genus Cynipencyrtus Ishii, (1928) (Gibson 2008, 2009), but Heraty et al. (2013) subsequently treated Cynipencyrtus as its own family, Cynipencyrtidae Trjapitzin, 1973. A second, well preserved female specimen of L. janzeni was then reported from Rovno amber by Simutnik et al. (2020a). Some of its character states, in particular a bare and flat prepectus, suggest that a separate family should likely also be established for Leptoomus. Sulia glaesaria Simutnik, 2015 was described from late Eocene Danish amber (Simutnik 2015b) and recently discovered in Rovno amber. Using a Leica Z16 APO stereomicroscope allowed revision of the diagnosis of this extinct genus. The earliest known Encyrtidae include one female and four males ascribed to five different genera from middle Eocene Sakhalinian amber (Simutnik 2014, 2015b, 2020; Simutnik et al. 2021). They differ significantly from modern and late Eocene European amber encyrtids. Material and methods Ukrainian Rovno amber (33.9-37.8 Mya) is coeval with Baltic and Danish ambers from which L. janzeni and S. glaesaria were described. The localities and composition of the Rovno amber fauna were recently character- ized in a series of reviews by Perkovsky et al. (2010), Perkovsky (2018), Martynova et al. (2019) and Mitov et al. (2021). Including new Pristocerinae and Epyrinae (Beth- ylidae) (Colombo et al. 2021a, b), Formica (Formicidae) (Radchenko et al. 2021) and the species reported here, 141 species of Hymenoptera are now known from Rovno amber, with 66 (47%) in common with Baltic amber. Nearly all studied Rovno amber inclusions from Rovno were collected from Klesov and the Horyn River Basin (Perkovsky et al. 2010; Perkovsky 2017; Kirichenko-Babko et al. 2021; Sukhomlyn et al. 2021; Mitov et al. 2021 and references therein) except those of new collections from the former Vladimirets and Zarechnoye districts of the Rovno region and Manevichi district of the Volyn region (basins of Styr, Veselukha and Stokhod rivers). These new collections (mostly from Kuchotskaya Volya, Voronki and Velyki Telkovichi) reveal dozens new species of hymenopterans, isopterans, blat- todeans, hemipterans, coleopterans, trichopterans, neuropterans and raphidiopterans (listed in Tshernyshev and Perkovsky (2021), with additions in Legalov et al. (2019, First record of the Sulia glaesaria Simutnik, 2015 from Rovno amber 87 2021), Gitka et al. (2021), Melnitsky et al. (2021), Jatoszynski and Perkovsky (2021), Golub et al. (2021) as well as species previously recorded from Baltic amber (Perkovsky and Olmi 2018; Martynova et al. 2019; Mamontov et al. 2020; Simutnik et al. 2020) or Baltic and Bitterfeld ambers (Radchenko and Perkovsky 2018, 2020). Danish amber is much understudied (Nadein et al. 2016). Its Hymenoptera was briefly reviewed by Simutnik et al. (2021). Sulia glaesaria is the first of four Danish encyrtid genera (Simutnik 2015b; Simutnik and Perkovsky 2017, 2018a, b; Simut- nik et al. 2020b, 2021) shared with another amber. Only two Dolichoderus (Formici- dae) species were previously known exclusively from Rovno and Danish amber, and Fallomyrma transversa Dlussky et Radchenko — from Rovno, Bitterfeld and Danish amber (Perkovsky 2018). The amber piece containing the new specimen of S. glaesaria was found near the village of Voronki in the Vladimirets District (basin of Styr River), Rovno Region, Ukraine. It is housed in the collection of the Schmalhausen Institute of Zoology of the National Academy of Sciences of Ukraine, Kiev (SIZK). Danish amber containing the holotype is reddish and not very transparent. The sample from Rovno amber is yellow and clear (Fig. 1A). The Rovno amber specimen is distinguished by a darker coloration of the body, antennae, legs, wing venation, and by having slightly infuscate, brownish, rather than hyaline forewings with narrow darkening ventral marginal vein and par- astigma, although this may be an artefact of differing amber preservation. It is smaller (1.7 mm vs 1.9 mm of holotype) but no differences were found in the proportions of its body parts. The condition of the Rovno specimen changed over time since it was initially studied, in one month (7.03.2021—13.04.2021) the air layer spreading over almost the entire mesoscutum (Fig. 5A, B), though since then until 7.07.2021 the extent of the air layer has not changed. Photographs were taken using Leica Z16 APO stereomicroscope equipped with a Leica DFC 450 camera and processed with LAS Core and Adobe Photoshop software (brightness and contrast only). Terminology and abbreviations follow Sharkov (1985), Gibson (1997), and Heraty et al. (2013). For the emended diagnosis, we used the characters listed by Noyes and Hayat (1994) in which the character states were polarized trough comparison with those of the Tanaostigmatidae; diagnosis of Tanaostigmatidae by Gibson (1989); the detailed analysis of the morphology of L. janzeni and comparison it with that of Cynipencyrtus Ishii, 1928 (Cynipencyrtidae), Encyrtidae, Eupelmidae, and Tanaostig- matidae (Gibson 2008); and lists of synapomorphies for the Chalcidoidea families of the clade E given by Heraty et al. (2013). The following abbreviations are used in the text and plates of illustrations: ac = acropleuron; acs = acropleural sulcus; ae = arched elevation of Mt6; btc = basitarsal comb; ¢ = cercus; cers = cercal setae; cly = clypeus; cs = covering setae of the linea calva; cuf = cubital fold; F1, F2, etc. = funicular segments 1, 2, etc.; hyp = hypopygium; Ic = linea calva; LOL = minimum distance between the anterior ocellus and a poste- rior ocellus; Is = line of setae along marginal vein of hind wing; mps = multiporous 88 Serguei A. Simutnik et al. / Journal of Hymenoptera Research 88: 85-102 (2021) plate sensilla; Mtl, Mt2, etc. = metasomal terga, numbering starts from petiole (Mt1); OOL = minimum distance between an eye margin and the adjacent posterior ocellus; OCL = minimum distance between a posterior ocellus and the occipital margin; opo = outer plates of ovipositor; p13 = metapleuron; POL = minimum distance between the posterior ocelli; pre = prepectus; r = radicle; sp = spiracle; spf = spur fold; spv = spur vein; st = strigil; syn = syntergum; vl +v2 = ovipositor stylet; v3 = ovipositor sheath. SIZK = I. I. Schmalhausen Institute of Zoology, National Academy of Sciences of Ukraine (Kyiv); ZMUC = Zoological Museum of the University of Copenhagen. Results Taxonomy Chalcidoidea Latreille, 1817 Family Encyrtidae Walker, 1837 Genus Sulia Simutnik, 2015 Type species. Sulia glaesaria Simutnik, 2015 (Simutnik 2015b, 2020). Material examined. Holotype, ZMUC, 5-1-1961, 9, Borge Mortensen, Dan- ish amber, late Eocene. The inclusion is in a reddish and not very transparent piece of amber in a shape of an almost rectangular parallelepiped (ca. 6 x 5 x 4 mm) (Fig. 1A). All body parts are preserved. Syninclusions absent. New material: SYZK, no. L-139, 19, vicinities of Voronki village, Vladimirets District, Rovno Region, Ukraine; Rovno amber; late Eocene. The inclusion is in a yel- low and clear piece of amber in a shape of triangular prism (ca. 20 x 16 x 15 x 7 mm) (Fig. 4A). All body parts are preserved. Syninclusions are absent. Diagnosis (emended). Body robust, barrel-shaped; clypeus setose, with long lat- eral margins and sharp ventrolateral angles; scape with apicoventral depression; funicle cylindrical; dorsoapical part of mesopleuron with convex differentiated region ventral to tegula delineated by sulcus; linea calva without filum spinosum, but with well- developed line of long setae along basal margin of dorsal surface; costal cell of hind wing with single line of long setae along the entire marginal vein; spur vein or fold originating from the marginal venation of the hind wing present but visible only at some angles; strigil and basitarsal comb present; gaster barrel-shaped, syntergum rather vertical with abruptly reflexed extension apically; cerci only slightly advanced toward gastral base; apex of hypopygium far from reaching apex of gaster; outer plates of ovi- positor narrow, crescent-shaped in lateral view. Redescription. Female. Largest known fossil encyrtid, body length 1.7-1.9 mm, not flattened, robust, barrel-shaped. Coloration. Head and body black, but appear silvery where surrounded by thin layer of air (only in these places a shallow reticular sculpture visible, Figs 2C, 3B, 4B, D, E, 5A, B, D). Scape black with lighter apex (holotype) or completely black (Rovno First record of the Sulia glaesaria Simutnik, 2015 from Rovno amber 89 Figure |. Sulia glaesaria, females A pieces of amber containing the inclusions: holotype in Danish amber, deposited in ZMUK (red) and new specimen from Rovno amber, deposited in SIZK (yellow) B head, holotype, ventrofrontal C habitus, holotype, right lateral (acs — acropleural sulcus, cly — clypeus; hyp — hypopygium, pre — prepectus). specimen); pedicel black; all segments of funicle from pale yellow (holotype) to entirely black (Rovno specimen); tegulae dark, legs of holotype lighter than those of Rovno specimen. Differences in color most likely an artefact of preservation. Head. Lenticular, as wide as thorax, broader then long, frontovertex curved to pos- terior ocelli; broadly rounded in frontal view, not vaulted above eyes; eyes bare, without 90 Serguei A. Simutnik et al. / Journal of Hymenoptera Research 88: 85-102 (2021) Figure 2. S. g/aesaria, female, holotype A antenna lateral B antenna dorsal C habitus, right posterolateral (c — cercus). visible microtrichia, inner orbits parallel over much of height but ventrally divergent (Figs 1B, 3C, 5D); frontovertex broader than long, minimum distance between eyes about 0.4x head width; ocelli normal in size, forming about 100° angle; OOL about equal to posterior ocellar diameter (Figs 3C, 5B); OOL:POL:LOL:OCL about 1.5:6:3.5:0.8; oc- cipital margin sharp; eye reaching occipital margin; antennal scrobe deep, not extended First record of the Sulia glaesaria Simutnik, 2015 from Rovno amber 91 Figure 3. S. g/aesaria, female, holotype A habitus, left lateral B habitus, ventral C head, frontal D fore- wing venation (cly — clypeus, hyp — hypopygium, Is — line of setae, opo — outer plates of ovipositor, p13 — metapleuron, r — radicle, sp — spiracle) number of mandibular teeth not visible; maxillary palpi 4-seg- mented; labial palpi 3-segmented (Fig. 5D). Scale bars: 0.5 mm (A=C). to anterior ocellus, in dorsal view anterior ocellus at equal distance from occiput margin and from margin of scrobal depression; interantennal projection small but visible in lateral view (Figs 1C, 2C); toruli located at level of lower margin of eyes (Figs 1B, 5D); distance between toruli equal to distance between lower margin of torulus and oral margin, about twice width of torulus, about 0.3x length of malar space; malar space with complete malar sulcus (Fig. 5D), slightly shorter than height of eye, about equal to width of mouth; clypeus widely emarginated with long lateral margins, about twice as broad as long, with sharp ventrolateral angles, and with row of setae (Figs 1B, 5D); mandibles wide (Figs 1B, 5D), Antenna. Geniculate, 11-segmented, without differentiated anelli, with 6 funicu- lar segments and with 3-segmented clava; radicle short, about 2—2.5x as long as broad 92 Serguei A. Simutnik et al. / Journal of Hymenoptera Research 88: 85-102 (2021) Figure 4. S. g/aesaria, female, new Rovno specimen A general view in piece of amber B dorsolateral C lateral D posterodorsal E dorsal F ventral G apex of mid leg. Scale bars: 0.5 mm (A-+F). (Fig. 1B); scape with apicoventral depression (Figs 2A, 3C), 2.7x as long as broad, wid- est at apex, flattened; pedicel conical, slightly shorter than first two funicular segments combined, longer than any segment of funicle; funicle cylindrical, FI-F3 longer than broad, F4—F6 almost square; all segments of funicle and clava with mps; clava as long as F4—F6 combined, without oblique truncation (Fig. 2A, B). Mesosoma. Pronotum almost vertical, but in dorsal view with very narrow transverse dorsal surface (Figs 1A, 3A, C), without medial line; mesoscutum not First record of the Sulia glaesaria Simutnik, 2015 from Rovno amber 93 a » Wa Figure 5. S. g/aesaria, female, Rovno specimen A head, mesosoma, dorsal B head, mesosoma with layer of air spread over mesoscutum, dorsal C head, antennae lateral D head, clypeus, mandibles, frontal. conspicuously wider than pronotum, broader than long, polygonally reticulate with sparse punctures, with posterior edge transverse, straight; mesoscutum articulated with scutellar-axillar complex only laterally, with edges of sclerites narrowly separated me- dially (Rovno specimen Figs 4D, 5A, B), connected by slender, delineated anterior portion of scutellar-axillar complex; mesothoracic spiracle open, not concealed be- neath pronotum (Fig. 3A); notauli absent, axillae transverse-triangular with antero- medial angles contiguous (Figs 4D, 5B); scutellum as broad as long, slightly convex, sculptured as mesoscutum and clothed in long, stout, black setae; prepectus large, bare, flat, with anterior margin not extend anterior to mesothoracic spiracle, posterior 94 Serguei A. Simutnik et al. / Journal of Hymenoptera Research 88: 85-102 (2021) margin extended to base of tegula (Figs 1C, 2C, 3A); acropleuron convex, bare, with longitudinal reticulate sculpture, short, in lateral view its length equal to height (Figs 1C, 2C, 3C), with distinct acropleural sulcus (Figs 1C, 2C) extended anteriorly from above base of mesocoxa toward posteroventral angle of prepectus; dorsoapical part of mesopleuron with small, convex differentiated region ventral to tegula, also delineated by distinct sulcus (Figs 1C, 2C, 3A), visible in holotype only; metapleuron triangular, narrow, without visible setation (Fig. 3A: pl3); propodeum bare, with large, polygo- nally reticulate lateral parts, and circular spiracle (Fig. 3A); mid coxa inserted level with mid line of mesopleuron (Fig. 2C), in ventral view with mesosternum transverse- rectangular and abutting bases of coxae, without a membranous area anterior to each coxal base; mesotarsal pegs arranged only along anteroventral margin of tarsus and coxae cannot rotate anteriorly out of their fossae (Fig. 4F). Wings. Fully developed. Fore wing with basal cell uniformly setose; costal cell broad; submarginal vein with single line of long setae, parastigma with two lines of shorter setae; cubital vein present as non-pigmented but distinct fold (Fig. 7B, cuf); linea calva almost entire, closed posteriorly by only one seta along cubital fold, with- out filum spinosum, but with well-developed line of long setae along basal margin of dorsal surface (Figs 3D, 7A, B: cs); parastigma only slightly widened, hyaline break (unpigmented area) present (Fig. 7B, C); marginal vein long, about 5 times as long as broad; stigmal vein slightly longer than marginal, with long narrow uncus and uncal sensilla; postmarginal vein twice as long as stigmal vein, enlarged seta marking apex of postmarginal vein of fore wing absent; setae of marginal fringe short. Hind wing relatively narrow; submarginal and marginal veins swollen, submarginal vein with sin- gle line of setae; costal cell narrow, membrane of cell along entire marginal vein with single line of long setae (Figs 3A, 6C, 7F, E: Is); spur vein originating from marginal venation present as differentiated hyaline (holotype) or pigmented (Rovno specimen Figs GC: spf; 7C—E: spv) process or fold (visible only at some angles); apex of marginal vein with 3 hamuli. Legs. Relatively short, stout; all coxae large, with polygonal reticulate sculpture (only visible under layer of air, Figs 4D, 5B); protibia with long, curved, bifurcate cal- car, with strong, straight, socketed seta and three stout setae along anteroapical margin (Fig. 6A); strigil and basitarsal comb present (Fig. GA); tarsi 5-segmented; mesotibia with thick mesotibial spur, about as long as mesobasitarsus (Figs 4G, 6B), and with row of pegs along anteroapical edge (Fig. 4G); ventral surface of mesobasitarsus and each next tarsomere with row of setae and irregular pattern of pegs along anteroventral edge (Fig. 6B); metatibia with two spurs. Gaster. Sculpture of gaster not visible, apical margins of Mt2 —Mt5 straight, par- allel; Mt6 with a small arched elevation medially (Figs 8B—D, 9A: ae); Mt7 U-like between cerci, almost vertical in both specimens but, perhaps, is an artefact of preser- vation resulting from metasoma being abnormally inflated due to decomposition ef- fects; Mt8, Mt9 fused as syntergum; syntergum also almost vertical except for abruptly reflexed extension apically (Figs 1A, 2C, 4C, 8A—D, 9A), setose and with two longer setae apicolaterally; cercus with long straight setae (Fig. 9A: cers), cerci only slightly First record of the Sulia glaesaria Simutnik, 2015 from Rovno amber 95 Figure 6. S. g/aesaria, female, Rovno specimen A front legs, ventral B apices of mid legs C venation of hind wing. (btc — basitarsal comb, spf — spur fold, st — strigil). Scale bars: 0.2 mm. advanced toward gastral base (Figs 2C, 8A—D), but gaster with elongate membranous band posterior to each cercus (Fig. 8A-C) differentiating dorsal surface of syntergum from slender lateral portion recurved around cercus; paratergites not visible; apex of hypopygium far from reaching apex of gaster (Figs 1C, 2C, 8A—C: hyp); ovipositor not extended beyond apex of ultimate tergum; only apical parts of ovipositor sheaths visible in ventrolateral and posterior views (Figs 8D, 9A: v3); membranous bands pos- terior to cerci subdivide the dorsal surface of syntergum from narrow, crescent-shaped in lateral view outer plates of ovipositor (Figs 3A, B, 8A, C, D, 9A: opo). Male. Unknown. Genus composition. Type species only. 96 Serguei A. Simutnik et al. / Journal of Hymenoptera Research 88: 85-102 (2021) Fs Gh, Mtoe : See To St: Sesthereth Pa —_ : : A, We Figure 7. S. g/aesaria, females, wings A—E Rovno specimen F holotype from Danish amber A left pair of wings dorsal B forewing venation, dorsal C left pair of wings, ventral D right pair of wings, venation, dorsal E left pair of wings, hind wing venation, ventral F wings venation, ventral. (cs — covering setae, cuf — cubital fold, lc — linea calva, Is — line of setae, spv — spur vein). Scale bars: 0.2 mm. Biology. Unknown. Comments. The radicle of many recent encyrtids is very long, four times or more as long as broad. This character state is included in lists of synapomorphies of extant Encyrtidae by Heraty et al. 2013, but there are many exceptions, among both extant Encyrtinae and Tetracneminae. The radicle of all known fossil Encyrtidae, including S. glaesaria, is also no more than twice as long as broad. The forewing venation of S. g/aesaria is typical for fossil Encyrtidae. In mod- ern encyrtids, the most similar venation of the forewings occurs, e.g., in the gen- era Savzdargia Trjapitzin, 1979, Ericydnus Walker, 1837, Mira Schellenberg, 1803, and Moraviella Hoffer, 1954 (Tetracneminae). All veins are long, including the First record of the Sulia glaesaria Simutnik, 2015 from Rovno amber 97 oventral D posterolateral. (ae — arched elevation of Mt6, c — cercus, syn — syntergum, v1+v2 — ovipositor stylet, v3 — ovipositor sheath). Scale bars: 0.2 mm. marginal. The linea calva has a line of long setae along its basal margin (Fig. 7B: cs), covering setae sensu Sharkov (1985). When the wings are folded, the ante- rior margin of one abuts this row of setae. This structure is present in most extant Encyrtinae, some extant Tetracneminae, and in all known earliest, middle Eocene encyrtids. Late Eocene encyrtids have been reported both with and without cover- ing setae (Simutnik 2020). In the hind wing of S. glaesaria, the costal cell has a membrane with a single line of long setae along the entire marginal vein as in Tanaostigmatidae. A similar char- acter state is rarely present in extant Encyrtidae, e.g., in Exoristobia Ashmead, 1904. The fossil E. sugonjaevi also has this line of setae, but it differs from S. g/aesaria by the hypopygium extending past the apex of horizontal syntergum. 98 Serguei A. Simutnik et al. / Journal of Hymenoptera Research 88: 85-102 (2021) Figure 9. Metasoma, lateral AS. g/aesaria, female, Rovno specimen B, C Prionomastix morio (Dalman, 1820) B female C male. (ae — arched elevation of Mt6, c — cercus, cers — cercal setae, syn — syntergum, vl+v2 — ovipositor stylet, v3 — ovipositor sheath). Scale bars: 0.2 mm. A spur vein or fold originating from the marginal venation of the hind wing is visible in S. glaesaria (Fig. 7C—E: spv) similar to that found in Tanaostigmatidae, Lep- toomus, some Pteromalidae, and Eupelmidae (Simutnik et al. 2020a). In S. glaesaria, however, the spur vein is present rather as a fold, visible only at some angles. The both specimens of S. glaesaria have almost vertical syntergum (Figs 2A, 9A). Perhaps, it is an artefact of preservation resulting from metasoma being abnormally First record of the Sulia glaesaria Simutnik, 2015 from Rovno amber 99 inflated due to decomposition effect. But, abruptly reflexed extension at the apex of syntergum (Figs 2A, 9A) indicates that it can actually be somewhat vertical in living specimens. The almost vertical syntergum is very rare in extant Encyrtidae. A similar structure is known, e.g., in Prionomastix Mayr, 1876 (Encyrtinae) (Fig. 9B, C), and S. glaesaria shares this character with many of members of Tanaostigmatidae. The taxonomic position of the Suw/ia is retain unplaced within the Encyrtidae. Its biology is also unknown. Nevertheless, a large size of S. glaesaria, its barrel-shaped habitus, short and high acropleuron, structure of protibial apex, presence of spur vein and line of long setae along the entire marginal vein of hind wings, and supposedly almost vertical syntergum somewhat resemble those of members of ‘Tanaostigmatidae and may indicate convergent adaptation to the plant galling. Acknowledgements We are grateful to Gary A.P. Gibson (Canadian National Collection of Insects and Arachnids) and James Woolley (Texas A8&cM University) for discussion and valued comments; Lars Vilhelmsen (Zoological Museum of University of Copenhagen) kind- ly arranged a loan of specimens; Bruce Archibald (Simon Fraser University, Burnaby, Canada) for valued comments and editing the English. The authors are thankful to the editor Petr Jansta. This work was supported by grant NRFU No. 2020/02/0369 (to S.A. Simutnik). References Colombo WD, Gobbi FT, Perkovsky EE, Azevedo CO (2021a) Synopsis of the fossil Pris- tocerinae (Hymenoptera, Bethylidae), with description of two new genera and species from Burmese, Taimyr, Baltic and Rovno ambers. Historical Biology 33(9): 1736-1752. https:// doi.org/10.1080/089 12963.2020.1733551 Colombo WD, Perkovsky EE, Waichert C, Azevedo CO (2021b) Synopsis of the fossil flat wasps Epyrinae (Hymenoptera, Bethylidae), with description of three new genera and 10 new species. Journal of Systematic Palaeontology 19(1): 39-89. https://doi.org/10.1080/ 14772019.2021.1882593 Gibson GAP (1989) Phylogeny and classification of Eupelmidae, with a revision of the world genera of Calosotinae and Metapelmatinae (Hymenoptera: Chalcidoidea). Memoirs of the Entomological Society of Canada 149: 1-121. https://doi.org/10.4039/entm121149fv Gibson GAP (1997) Chapter 2, Morphology and terminology. In: Gibson GAP, Huber JT, Woolley JB (Eds) Annotated keys to the genera of Nearctic Chalcidoidea (Hymenoptera). NRC Research Press, Ottawa, 16-45. [794 pp.] Gibson GAP (2008) Description of Leptoomus janzeni, n. gen. and n. sp. (Hymenoptera: Chalcidoidea) from Baltic amber, and discussion of its relationships and classification rela- tive to Eupelmidae, Tanaostigmatidae and Encyrtidae. Zootaxa 1730: 1-26. https://doi. org/10.11646/zootaxa.1730.1.1 100 Serguei A. Simutnik et al. / Journal of Hymenoptera Research 88: 85-102 (2021) Gibson GAP (2009) Description of three new genera and four new species of Neanastatinae (Hy- menoptera, Eupelmidae) from Baltic amber, with discussion of their relationships to extant taxa. In: Johnson N (Ed.) Advances in the systematics of Hymenoptera. Festschrift in hon- our of Lubomir Masner. ZooKeys 20: 175-214. https://doi.org/10.3897/zookeys.20.161 Gilka W, Harbach RE, Perkovsky EE (2021) Mosquitoes (Diptera: Culicidae) in Eocene amber from the Rovno region, Ukraine. Zootaxa 5016(2): 257-270. https://doi.org/10.11646/ zootaxa.5016.2.6 Golub VB, Perkovsky EE, Vasilenko DV (2021) A new fossil species of the genus Parasinalda Heiss & Golub (Hemiptera: Heteroptera: Tingidae) from Upper Eocene Rovno amber. Zootaxa 5027(2): 290-296. https://doi.org/10.11646/zootaxa.5027.2.9 Heraty JM, Burks RA, Cruaud A, Gibson GA, Liljeblad J, Munro J, Rasplus JY, Delvare G, Jansta PR, Gumovsky A, Huber JT, Woolley JB, Krogmann L, Heydon S, Polaszek A, Schmidt S, Darling DC, Gates MW, Mottern J, Murray E, Dal Molin A, Triapitsyn S, Baur H, Pin- to JD, van Noort S, George J, Yoder M (2013) A phylogenetic analysis of the megadiverse Chalcidoidea (Hymenoptera). Cladistics 29: 466-542. https://doi.org/10.1111/cla.12006 Jaloszynski P, Perkovsky EE (2016) Diversity of Scydmaeninae (Coleoptera: Staphylinidae) in Upper Eocene Rovno amber. Zootaxa 4157(1): 1-85. https://doi.org/10.11646/ zootaxa.4157.1.1 Jatoszytiski P, Perkovsky EE (2021) A new bizarre species of Euconnus (Cladoconnus) in Upper Eocene Rovno amber (Coleoptera: Staphylinidae: Scydmaeninae). Zootaxa 5004(2): 395— 400. https://doi.org/10.11646/zootaxa.5004.2.8 Kirichenko-Babko M, Perkovsky EE, Vasilenko DV (2021) A new genus and species of Lebiini (Coleoptera: Carabidae) from late Eocene Rovno amber. Historical Biology. https://doi.or g/10.1080/08912963.2021.1924701 Legalov AA, Nazarenko VYu, Perkovsky EE (2019) New weevils (Coleoptera: Curculionidae) from Rovno amber. Paleontological Journal 53(10): 1045-1059. https://doi.org/10.1134/ S$0031030119100101 Legalov AA, Nazarenko VYu, Vasilenko DV, Perkovsky EE (2021) Ceutorhynchus Germar (Coleoptera, Curculionidae) as proxy for Eocene core Brassicaceae: first record of the genus from Rovno amber. Journal of Paleontology. https://doi.org/10.1017/jpa.2021.82 Mamontov YuS, Atwood JJ, Perkovsky EE, Ignatov MS (2020) Hepatics from Rovno amber (Ukraine): Frullania pycnoclada and a new species, FE vanae. The Bryologist 123(3): 421- 430. https://doi.org/10.1639/0007-2745-123.3.421 Martynova KV, Perkovsky EE, Olmi M, Vasilenko DV (2019) New records of Upper Eocene chrysi- doid wasps (Hymenoptera: Chrysidoidea) from basins of Styr and Stokhod rivers (Rovno amber). Paleontological Journal 53(10): 998-1023. https://doi.org/10.1134/S0031030119100125 Melnitsky SI, Ivanov VD, Perkovsky EE (2021) A new species of Plectrocnemia (Trichop- tera: Polycentropodidae) from Rovno amber. Zootaxa 5006(1): 106-109. https://doi. org/10.11646/zootaxa.5016.2.5 Mitov PG, Perkovsky EE, Dunlop JA (2021) Harvestmen (Arachnida: Opiliones) in Eocene Rov- no amber (Ukraine). Zootaxa 4984: 43-72. https://doi.org/10.11646/zootaxa.4984. 1.6 Nadein KS, Perkovsky EE, Moseyko AG (2016) New Late Eocene Chrysomelidae (Insecta: Coleoptera) from Baltic, Rovno and Danish ambers. Papers in Palaeontology 2: 117-137. https://doi.org/10.1002/spp2.1034 First record of the Sulia glaesaria Simutnik, 2015 from Rovno amber 101 Noyes JS, Hayat M (1994) Oriental mealybug parasitoids of the Anagyrini (Hymenoptera: Encyrtidae). CAB International, Wallingford, Oxon, [viii+] 554 pp. Perkovsky EE (2016) Tropical and Holarctic ants in Late Eocene ambers. Vestnik zoologii 50(2): 111-122. https://doi.org/10.1515/vzoo-2016-0014 Perkovsky EE (2017) Rovno amber caddisflies (Insecta, Trichoptera) from different locali- ties, with information about three new sites. Vestnik zoologii 51(1): 15-22. https://doi. org/10.1515/vzoo-2017-0003 Perkovsky EE (2018) Only a half of species of Hymenoptera in Rovno amber is common with Baltic amber. Vestnik zoologii 52(5): 353-360. https://doi.org/10.2478/vzoo-2018-0037 Perkovsky EE, Olmi M (2018) Discovery of the first pincer wasp (Hymenoptera, Dryinidae) from Rovno amber. Zootaxa 4457(2): 296-304. https://doi.org/10.11646/zootaxa.4457.2.5 Perkovsky EE, Zosimovich VYu, Vlaskin AP (2010) Rovno amber. In: Penney D (Ed.) Biodi- versity of Fossils in Amber from the Major World Deposits. Siri Sciences Press, Manches- ter, 116-136. Peters R, Niehui O, Gunkel S, Blaser M, Mayer C, Podsiadlowski L, Kozlov A, Donath A, van Noort S, Liu Sh, Zhou X, Misof B, Heraty J, Krogmann L (2018) Transcriptome sequence-based phylogeny of chalcidoid wasps (Hymenoptera: Chalcidoidea) reveals a his- tory of rapid radiations, convergence, and evolutionary success. Molecular Phylogenetics and Evolution 120: 286-296. https://doi.org/10.1016/j.ympev.2017.12.005 Radchenko AG, Perkovsky EE (2018) First record of fossil ant species Eocenomyrma rugosos- triata (Mayr) (Hymenoptera: Formicidae) from the Rovno amber. Russian Entomological Journal 27(3): 285-288. https://doi.org/10.15298/rusentj.27.3.08 Radchenko AG, Perkovsky EE (2020) New records of the fossil ant genus Prionomyrmex Mayr (Hymenoptera, Formicidae, Myrmeciinae) from Late Eocene European ambers. Paleon- tologicheskii Zhurnal 2020(6): 60-67. [In Russian] [Translated: Paleontological Journal 54(6): 617-626] https://doi.org/10.1134/S003 1030120060088 Sharkov AV (1985) Encyrtids (Hymenoptera, Chalcidoidea, Encyrtidae) of the southern Far East of the USSR [dissertation]. Extended Abstract of Cand. Sci. (Biol.). Leningrad. [in Russian] Simutnik SA (2001) A find of encyrtid (Hymenoptera, Chalcidoidea, Encyrtidae) in the late Eocene Rovno amber. Vestnik Zoologii 35(6): 81-84. [in Russian] Simutnik SA (2002) A new genus of encyrtid wasps (Hymenoptera, Chalcidoidea, Encyrtidae) from the late Eocene Rovno amber (Ukraine). Vestnik Zoologii 36(4): 99-102. [in Russian] Simutnik SA (2007) Fossil Encyrtidae (Hymenoptera: Chalcidoidea) from the Rovno amber. Izvestiya Kharkovskogo Entomologicheskogo Obshchestva 15(1—2): 137-141. [in Russian] Simutnik SA (2014) First record of Encyrtidae (Hymenoptera, Chalcidoidea) from the Sakha- lin Amber. Paleontologicheskii Zhurnal 2014(6): 46-49. [In Russian] [Translated: Paleon- tological Journal 48(6): 621-623] https://doi.org/10.1134/S0031030114060124 Simutnik SA (2015a) A new fossil genus of Encyrtidae (Hymenoptera: Chalcidoidea) from late Eocene Danish amber. Russian Entomological Journal 24(1): 73-75. https://doi. org/10.15298/rusentj.24. 1.07 Simutnik SA (2015b) Description of two new monotypical genera of encyrtid wasps (Hymenop- tera, Chalcidoidea: Encyrtidae), based on males from the middle Eocene Sakhalin amber. Entomological Review 95(7): 937-940. https://doi-org/10.1134/S0013873815070118 102 Serguei A. Simutnik et al. / Journal of Hymenoptera Research 88: 85-102 (2021) Simutnik SA (2020) The earliest Encyrtidae (Hymenoptera, Chalcidoidea). Historical Biology. https://doi.org/10.1080/08912963.2020.1835887 Simutnik SA, Perkovsky EE (2017) Protocopidosoma gen. nov. (Hymenoptera, Chalcidoidea, Encyrtidae) from the late Eocene Danish amber. Paleontologicheskii Zhurnal 2017(3): 64-65. [In Russian] [Translated: Paleontological Journal 51(3): 288-290] https://doi. org/10.1134/S0031030117030108 Simutnik SA, Perkovsky EE (2018a) Dencyrtus gen. nov. (Hymenoptera, Chalcidoidea, Encyrti- dae) from the late Eocene Danish amber. Paleontologicheskii Zhurnal 2018 (3): 67-68. [In Russian] [Translated: Paleontological Journal 52(3): 290-293] https://doi.org/10.1134/ S$0031030118030139 Simutnik SA, Perkovsky EE (2018b) Trjapitzion Simutnik, gen. n. (Hymenoptera, Chalci- doidea: Encyrtidae), a new genus of encyrtid wasps from the late Eocene Rovno amber. Entomological Review 98(8): 1152-1156. https://doi.org/10.1134/S0013873818080225 Simutnik SA, Perkovsky EE (2018c) Archaeocercus gen. nov. (Hymenoptera, Chalcidoidea, En- cyrtidae) from late Eocene Rovno amber. Zootaxa 4441 (3): 543-548. https://doi.org/10. 1080/08912963.2020.1835887 Simutnik SA, Perkovsky EE (2020) Ektopicercus Simutnik gen. nov. (Hymenoptera, Chalci- doidea, Encyrtidae) from late Eocene Rovno amber. Palaeoentomology 3(4): 342-346. https://doi.org/10.11646/palaeoentomology.3.4.3 Simutnik SA, Perkovsky EE, Gumovsky AV (2014) Review of the Late Eocene Encyrtidae (Hy- menoptera, Chalcidoidea) with a description of the first fossil genus with filum spinosum. Paleontologicheskii Zhurnal 2014(1): 65-74. [In Russian] [Translated: Paleontological Journal 48(1): 65-73] https://doi.org/10.1134/S0031030114010122 Simutnik SA, Perkovsky EE, Vasilenko DV (2020a) First record of Leptoomus janzeni Gibson (Hymenoptera, Chalcidoidea) from Rovno amber. Journal of Hymenoptera Research 80: 137-145. https://doi.org/10.3897/jhr.80.58882 Simutnik SA, Perkovsky EE, Vasilenko DV (2020b) Efesus trufanovi Simutnik gen. et sp. n. (Hymenoptera: Chalcidoidea: Encyrtidae) from late Eocene Danish amber. Russian Entomological Journal 29(3): 298-302. https://doi.org/10.15298/rusentj.29.3.10 Simutnik SA, Perkovsky EE, Vasilenko DV (2021) Sakhalinencyrtus leleji Simutnik gen. et sp. nov. of earliest Encyrtidae (Hymenoptera, Chalcidoidea) from Sakhalinian amber. Journal of Hymenoptera Research 84: 361-372. https://doi.org/10.3897/jhr.84.66367 Sukhomlyn MM, Heluta VP, Perkovsky EE, Ignatov MS, Vasilenko DV (2021) First record of a fungus of Mycocaliciaceae from Rovno amber (Ukraine). Paleontologicheskii Zhurnal 2021(6): 98-103. [In Russian] [Translated: Paleontological Journal 55(6): 684-690] Trjapitzin VA (1989) Parasitic Hymenoptera of the fam. Encyrtidae of Palaearctics. Opredeliteli po faune SSSR izdavaemiye Zoologicheskim institutom AN SSSR 158: 1-489. [In Russian] Tshernyshev SE, Perkovsky EE (2021) Protomauroania mikhailovi — a new species of malachite beetles (Coleoptera, Dasytidae) in Rovno amber. Zootaxa 5006(1): 189-194. https://doi. org/10.11646/zootaxa.5006.1.20 Zhang J, Lindsey ARI, Peters RS, Heraty JM, Hopper KR, Werren JH, Martinson EO, Woolley JB, Yoder MJ, Krogmann L (2020) Conflicting signal in transcriptomic markers leads to a poorly resolved backbone phylogeny of chalcidoid wasps. Systematic Entomology 45(4): 783-802. https://doi.org/10.1111/syen.12427