Lavender essential oil is widely used in pharmacy, perfumery and the food industry. It is one of the key essential oils in aromatherapy due to its valuable pharmacological properties. The producers of lavender essential oil are well aware that the greatest quantity of oil is obtained near the end of the inflorescence anthesis and that oil quantity is correlated with the pollination as unpollinated flowers drop down. In addition, it has been demonstrated that oil quality is also highest at the end of the flowering period, related to the gradual increase of monoterpenes (particularly the valuable linalool) and the decrease of sesquiterpenes during flower ontogenesis. The aim of this preliminary study was to measure the occurrence of spontaneous self-pollination in Lavandula angustifolia Mill. and to identify external pollinators. The field experiments were performed in a lavender plantation near Gorna Lipnitza Village, north Bulgaria and in the ex-situ lavender collection in the experimental plot of the Botanical Garden of Sofia University. It was revealed that spontaneous self-pollination did not occur in flowers from which external pollinators had been excluded. Exposed flowers were pollinated by polylectic insects, such as honeybees, several species of bumblebees and butterflies. Wild pollinators (particularly bumblebees) dominated over honeybees at both study sites. Our observations showed that all pollinators actively collected nectar. The pollen baskets of most bees were full, indicating the active consolidation of pollen adhering to the pollinators’ bodies. Although lavender growers tend to place beehives in the fields for optimal essential oil production, it is also crucial to conserve wild pollinators.