The family Deuterophlebiidae is considered the most plesiomorphic Diptera family, with striking morphology and life history. In this study, we provide detailed descriptions and figures of two new species from Southwestern China: Deuterophlebia pseudopoda sp. nov. and Deuterophlebia pachychaeta sp. nov., along with genetic distances of COI sequences between the seven known Chinese species of Deuterophlebia Edwards, 1922. The two new species can be identified by the adults’ male terminalia, head structures, male and female antennae and mesothoracic spines or abdominal projections of the pupae. As a result, China is currently the country with the highest Deuterophlebia species diversity.