The adult, pupa, and larva of three new species in the genus Culex, subgenus Culex, are described and illustrated. Adults of Culex pseudostigmatosoma n. sp. are highly ornamented and similar to Cx. stigmatosoma Dyar. Culex pseudostigmatosoma is known to occur from Mexico to Panama. Adults of Culex yojoae n. sp. are moderately ornamented and similar to Cx. coronator Dyar and Knab. Culex yojoae is known only from its type locality in central Honduras. Adults of Culex aquarius n. sp. are unornamented and similar to many species in the region. It has been collected in Costa Rica and Panama. The larvae of the three species are similar to those of Cx. chidesteri Dyar (illustrated from confirmed material). The four similar larvae are separated in a key.