A new species, Anopheles (Nys.) trinkae Faran, in the Oswaldoi complex of the Albimanus section is described and illustrated. Anopheles trinkae has been collected along the eastern slope of the Andes from Villavicencio, Colombia, south to Puyo, Ecuador, in a variety of ground pool-lake-stream margin habitats. Anopheles trinkae is most closely allied to An. nuneztovari and together these 2 species form the sister group of An. rangeli.