Deficiencies, induced by radiation, were use to map four mutant loci on the salivary gland chromosomes of Anopheles albimanus. The loci for Red eye and Ebony were determined to be in Regions 10C and 20A, respectively, on chromosome 2. Reduced palmate and Stripe were assigned to 30B and 33B, respectively, on chromosome 3. During the screening for deficiency types, a total of 272,233 F1 progeny, from irradiated males, were examined, and 147 larvae had the genotype indicating a deficiency. Upon examination of the salivary gland chromosomes, visible deficiencies were recorded for 38 larvae.