Eighty new tires were placed in field habitats, half in a junkyard and half in a woodlot. A 300 ml sample of water was taken from alternate tires once weekly for a 12 week period. There were 17,173 larvae collected from 8 different habitats, 90% of the larvae were Culex restuans, the most rapid (7 days) and most successful colonizer of new habitats. Aedes atropalpus was more common in tires in the open and Ae. triseriatus was more common in the shade. Ae. atropalpus proved to be a more rapid colonizer than Ae. triseriatus (17 days vs. 31 days), probably because of the tendency to oviposit directly on the water surface. The presence of leaf litter did not affect oviposition choice by either Aedes species. Aedes triseriatus was shown to be bivoltine in northern Indiana. A total of 2,261 adult mosquitoes in 13 species was collected from tires by suction aspirator during the 14 week summer season. Among these were 190 females and 125 males of Ae. atropalpus; about 67% of the Ae. atropalpus came from exposed, unshaded tires.