Twenty-four species of mosquitoes were collected in Nemaha County of Southeastern Nebraska. Eleven species were collected for the first time in the county including Aedes canadensis, Ae. cinereus, Ae. Sollicitans, Ae. triseriatus, Anopheles barberi, An. quadrimaculatus, Culex territans, Culiseta inornata, Orthopodomyia signifera, Psorophora cyanescens and Ps. horrida. Aedes vexans was the most abundant adult collected in biting and light trap surveys. This species made up 67% of the total catch. Aedes vexans was followed in prominence by Ae. triseriatus (11%), Ae. trivittatus (10%), An. punctipennis (4%) and Cs. inornata (3%).