This paper describes the techniques for establishing and maintaining a laboratory stain of Culex (Melanoconion) taeniopus from Guatemala. Developmental times, survival rates and other data pertaining to the population dynamics and behavior of this important vector are presented. It was concluded that mating took place during the crepuscular periods 6-10 days after emergence. Females survived for approximately three months and males for two months. Oogenesis was completed as early as 96 hours after the initial bloodmeal. Oviposition did not begin before day 5 and continued beyond 18 days after the bloodmeal. Egg rafts were deposited on the inside of clay flower pots, and hatched in approximately 48 hr. The overall larval survival rate was 82.3%, averaging approximately 13 days for development from eclosion to pupation. The average pupal developmental time was approximately 59 hr with a 92.4% survival. Cross-mating studies between this Guatemalan strain and a laboratory strain from Florida indicate that the two are distinct species.