To confirm or refute nocturnal blood-feeding from cattle by Culicoides venustus, haematophagous insects attracted to pastured calves were sampled through 5 nights. Drop-trap and vacuum aspirator samples were taken at 30 min before and after sunset and at hourly intervals through the night until an hour after sunrise. Female C. venustus were consistently captured in drop-trap samples during darkness and sunrise twilight; only 1 specimen was captured during sunset twilight. Vacuum samples captured C. venustus sporadically during darkness. The data confirm that female C. venustus are primarily nocturnal in host-seeking and consistently utilize cattle as a blood meal source.