A simple, reliable technique was developed for rabid colonization of Anopheles albimanus Wiedemann. Field collected adult females were placed in plastic vials containing water to induce oviposition. Subsequent generations of adults were then placed in colony cages for mating and oviposition. The 500 parental females produced 9009 F1 females, which laid >200,000 eggs. An equal number of field-collected females placed in a cage for oviposition produced only 62 F1 females, which failed to oviposit.