Host-seeking females of Simulium jenningsi were collected in 7 sequential morning and afternoon samples in the Champlain Valley of New York state. All flies in each collection were dissected to determine 1) parity, 2) the presence of spermatozoa in the spermathecae, and 3) the presence of nectars in the crop. Parous flies were further separated according to the state of the follicular relics. Chi-square analysis confirmed the presence of a distinct biting cycle in that nullipars predominated in the morning (60%) and parous flies predominated in the afternoon (67%. Parous flies captured in the afternoon most frequently exhibited following follicular relics in the sac stage, suggesting that oviposition probably occurs early in the morning or late in the previous evening. All flies were mated and nearly all (94%) had nectar fed. No evidence of autogeny was noted.