Two colonies of Anopheles pseudopunctipennis pseudopunctipennis Theobald have been established in El Salvador using induced copulation. The Ilopango strain was initiated using immatures collected from a highland lake. The Huiza strain was started with blooded females captured near a coastal river. A study of stenogamy began with holding male and female adults together in emergence cages. Natural mating has been achieved and oviposition of viable eggs has continued on a substrate of moist earth wetted with larval essence.