Aedes triseriatus mosquitoes collected from tires and treeholes in New York, and those from an established colony in Maryland (Gerberg) were reared in the laboratory. Eggs from each of these 3 colonies were conditioned at 80% RH, 26 C and flooded weekly. Females from the established colony and those from tires were found more aggressive and efficient in feeding than those from treeholes, and their eggs were deposited in clusters of more than 100 and hatched rapidly after 4-5 weeks of conditioning. In contrast, the treehole strain females deposited less than 30 eggs per cluster which hatched intermittently for over a 13 week period.