Larval collections in Nebraska during 2000 established 2 new state distribution records for Ochlerotatus epactius and Anopheles perplexens and confirmed the presence of Culiseta melanura, bringing the total number of mosquito species reported from Nebraska to 50. Fifteen new county records for 10 species are noted, including the 1st report of Aedes albopictus from Lancaster County, marking its westernmost collection in Nebraska to date.