A tent treated with an oil-based 1% permethrin formulation (0.936 g AI/m2) was evaluated for protection against Aedes spp. Mosquito biting assessment was conducted in the field using 8 human subjects positioned inside and outside tents. Subjects inside tents received 66.8% fewer bites than subjects outside tents prior to treatment. Following treatment, subjects inside the treated tent had significant (P < or = 0.05) protection (84-94% bite reduction) for 42 days. Subjects outside the treated tent experienced 43-82% fewer biting mosquitoes than subjects outside the untreated tent. The treated tent remained lethal > or = 42 days to mosquitoes exposed to the tent fabric in field bioassays.