Simple techniques are described for collecting black fly pupae from streams using plastic strips and for rearing large numbers of adult black flies in inexpensive enclosures made of chicken wire and cloth mesh netting, and 2 methods are described for rearing adult black flies individually from pupae. The first method of rearing individual black flies uses 1.5-ml microcentrifuge tubules and the second uses easy-to-construct rearing chambers that provide moisture for the developing pupa and water for the adult to imbibe. Instructions for assembly are provided. Specimens obtained using these rearing chambers are of museum quality.