Studies of host selection patterns of Anopheles pseudopunctipennis were conducted in villages in foothills near Tapachula, Mexico. Based on 2 years of collections, 53.8 and 86.1% of all engorged females resting inside houses were found to contain human blood. Estimates of weighted and unweighted human blood indices, including data from outdoor resting collections, varied from 29.5 to 54.7%. Humans and dogs were the more common blood sources for all An. pseudopunctipennis mosquitoes, accounting for 96% of blood meals tested. Results of analyses of host preference through estimates of forage ratios (FRs) indicated that the large numbers of blood meals from humans and dogs were more reflective of host availability than host preference. An FR of less than 1 indicated that, in terms of host availability, proportionately fewer An. pseudopunctipennis females fed on humans than other large animal hosts. In contrast, FRs of 15-20 and 5-7 revealed strong selective biases for horses and pigs as sources of blood meals, respectively. The proportion of outdoor-resting, blood-engorged females containing human blood declined markedly after houses were sprayed with DDT. This response to house spraying is attributed to an excito-repellency effect of DDT.