Effectiveness of sustained-release Altosid pellets (4% AI methoprene) against floodwater mosquitoes in dambos treated at 5, 3, and 1 wk before and 1 day after flooding was determined. Only 2% of Aedes pupae (primarily Aedes mcintoshi) survived to adults in an area treated 5 wk preflood, and no adult mosquitoes emerged from an area treated 1 day after flooding. In contrast, 12 and 16% of Aedes pupae successfully survived to the adult stage in areas pretreated 3 and 1 wk, respectively, preflood. The effectiveness of the Altosid declined against Culex spp. (primarily Cx. antennatus) collected from dambos 15-31 days after flooding. The potential for using preflood treatment with methoprene to control Aedes vectors of Rift Valley fever virus in endemic areas is discussed.