Twenty-four species of Culicoides were collected between April and September of 1986 from Nemaha and Richardson counties of southeastern Nebraska using New Jersey light traps and tree hole sampling. Light trap samples were dominated by C. crepuscularis (42.4%), C. stellifer (29.6%), C. haematopotus (17.4%), C. variipennis (4.1%) and C. obsoletus (2.8%). Culicoides guttipennis and C. villosipennis were the most common of the 9 tree hole species collected. New state records for Nebraska were obtained for C. lahillei, C. riggsi, C. footei, C. paraensis, C. nanus, C. spinosus, C. biguttatus and C. piliferus.