The establishment of a free-mating colony of Anopheles albitarsis sensu siricto, a member of the Anopheles albitarsis complex, is described. Groups of females from the F5, F6, and F11 generations were examined, and the percent inseminated, mean number of eggs oviposited, and percent hatch discussed. The colony has been continued through 18 generations, with larval development averaging 10 days, and larval mortality ranging between 20 and 30%.